Diabetes and brain: omics approaches to study diabetic encephalopathy DOI Creative Commons

Nicoletta Lionetti,

M LAGO,

T Brescia

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: May 12, 2025

Diabetes mellitus (DM) is a complex metabolic disorder associated with many complications, including diabetic encephalopathy (DE). DE severe neurological condition characterized by progressive decline in cognitive and motor functions, significantly impacting patients’ quality of life. Despite advancements understanding DM, the intricate pathogenetic mechanisms underlying remain incompletely elucidated. This review comprehensively analyzes application omics technologies to decipher molecular basis identify potential diagnostic biomarkers therapeutic targets. Several studies on animal models have revealed specific signatures changes gene expression key memory brain regions, like hippocampus, highlighting We explore how these “omics” approaches provided novel insights into interplay factors contributing DE. Recurrent alterations were identified upon evaluation analysis from human tissues vitro Findings indicate that this pathological impaired energy metabolism, oxidative stress, neuroinflammation, neuroendocrine dysfunction influence gut microbiota. A multi-omics approach, integrating data various limited studies, enhances translational pathogenesis, new implications for diagnosis treatment.

Language: Английский

Huang-Lian-Jie-Du decoction alleviates cognitive deficits in Alzheimer’s disease model 5xFAD mice by inhibiting Trem2/Dap12 signaling pathway DOI Creative Commons
Ran Pang, Jia Shi, Xiaoyu Peng

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 15, 2025

Abstract Background Alzheimer’s disease (AD) is a progressive neurodegenerative disorder predominantly affecting the elderly population. It characterized by cognitive deficits associated with accumulation of amyloid-beta plaques and neurofibrillary tangles. Huang-Lian-Jie-Du (HLJD) decoction, recognized as representative formulation heat-clearing detoxification effects, has been demonstrated to be effective in treating AD. However, underlying mechanisms require further investigation. Methods 5xFAD mice were administrated low high doses HLJD. The Morris water maze test was conducted assess effects Aβ42 total tau protein levels evaluated. Additionally, network pharmacology analysis performed identify therapeutic targets HLJD’s active components their relevance ELISA, qPCR, Western Blot, immunofluorescence assays employed confirm identified pathways. Finally, primary microglia isolated from used validate candidate Results HLJD improved reduced amyloid plaque deposition levels. Network indicated that influences neuroinflammatory response, particularly through Dap12 signaling pathway. This confirmed neuroinflammation markers, including TNF-α, IL-1β, IL-6, indicators microglial activation polarization. expression Trem2 hippocampus (HIP) mice, well microglia, downregulated following treatment. Conclusion Our study indicates alleviates AD suppressing Trem2/Dap12 pathway HIP thereby inhibiting neuroinflammation. Graphical

Language: Английский

Citations

0
Diabetes and brain: omics approaches to study diabetic encephalopathy DOI Creative Commons

Nicoletta Lionetti,

M LAGO,

T Brescia

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: May 12, 2025

Diabetes mellitus (DM) is a complex metabolic disorder associated with many complications, including diabetic encephalopathy (DE). DE severe neurological condition characterized by progressive decline in cognitive and motor functions, significantly impacting patients’ quality of life. Despite advancements understanding DM, the intricate pathogenetic mechanisms underlying remain incompletely elucidated. This review comprehensively analyzes application omics technologies to decipher molecular basis identify potential diagnostic biomarkers therapeutic targets. Several studies on animal models have revealed specific signatures changes gene expression key memory brain regions, like hippocampus, highlighting We explore how these “omics” approaches provided novel insights into interplay factors contributing DE. Recurrent alterations were identified upon evaluation analysis from human tissues vitro Findings indicate that this pathological impaired energy metabolism, oxidative stress, neuroinflammation, neuroendocrine dysfunction influence gut microbiota. A multi-omics approach, integrating data various limited studies, enhances translational pathogenesis, new implications for diagnosis treatment.

Language: Английский

Citations

0