Characterization of a novel lytic phage vB_AbaM_AB4P2 encoding depolymerase and its application in eliminating biofilms formed by Acinetobacter baumannii

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 8, 2025

Acinetobacter baumannii strains are a primary cause of hospital-acquired infections. This bacterium frequently causes biofilm-related infections, notably ventilator-associated pneumonia and catheter-related which exhibit remarkable resistance to antibiotic treatment, posing severe challenge in the prevention A. Therefore, strategies eliminate biofilm catheters becoming increasingly important. Phages capable lysing bacteria have certain effect on ablation biofilms. Sewage treatment plant water was collected for isolation phages. The morphological, host range, one-step growth, temperature pH stability, bactericidal activity, sequencing genomic analysis were performed characterize isolated phage. three-dimensional structure tail fiber protein predicted by AlphaFold3. efficacy phage clearing biofilms from 24-well plates PVC also evaluated. In this study, lytic vB_AbaM_AB4P2 sewage water, showing clear plaque with halo zone. One-step growth assays unveiled 20-minute latent period burst size 61 forming unit/cell (PFU/cell). At same time, AB4P2 exhibited stability at 3–11 temperatures 30–70 °C. Its dsDNA genome is composed 45,680 bp G + C content 46.13%. Genomic phylogenetic situated as new species Caudoviricetes class. possesses pectin lyase-like domain that linked depolymerase playing crucial role disrupting Additionally, it encodes lysis cassette comprising endolysin, holin Rz-like spanin, yet lacks any genes responsible virulence factors. Phage can completely inhibit 16 h. plate polyvinyl chloride (PVC) catheter model experiments, achieved significant rate effectively killed live bacterial cells biofilm. had good environmental strong ability destroy formed baumannii. It exhibits promising potential development an alternative disinfectant against hospital. Not applicable.

Language: Английский

Reviving hope: Phage therapy application for antimicrobial resistance in farm animal production over the past decade

Animal Feed Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 116333 - 116333

Published: April 1, 2025

Language: Английский

Isolation and Genomic Analysis of Escherichia coli Phage AUBRB02: Implications for Phage Therapy in Lebanon

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 458 - 458

Published: April 30, 2025

Background/Objectives: Escherichia coli (E. coli), a prevalent Gram-negative bacterium, is frequent cause of illness. The extensive use antibiotics has led to the emergence resistant strains, complicating antimicrobial therapy and emphasizing need for natural alternatives such as phages. Methods: In this study, novel phage, AUBRB02, was isolated from sewage characterized through whole-genome sequencing, host range assays, biofilm elimination assays. phage’s stability infectivity were assessed under various pH temperature conditions, different E. strains. Results: Phage AUBRB02 an incubation period 45 min, lysis 10 burst size 30 phages/infected cell. It stable across 5.0–9.0 temperatures 4 °C 60 °C. Treatment with significantly reduced post-formation biofilms, indicated by lower OD values compared positive control. whole genome sequencing revealed 166,871 base pairs G + C (Guanine Cytosine content) content 35.47%. belongs Tequatrovirus genus, sharing 93% intergenomic similarity its closest RefSeq relative, encodes 262 coding sequences, including tRNAs. Conclusions: demonstrates high diverse conditions. Its genomic features related phages highlight potential phage therapy, offering promising prospects targeted treatment infections.

Language: Английский