Phytochemical Profiling by HPLC‐ESI‐MS/MS and In Vitro Investigation of the Antidiabetic Activity of Cassia bakeriana Bark Extract and Fractions DOI Open Access
Diego Godina Prado, Allisson Benatti Justino,

Tiara da Costa Silva

et al.

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(12)

Published: Nov. 15, 2024

ABSTRACT Type 2 diabetes mellitus is a global health problem, placing patients at higher risk of developing cardiovascular diseases and cancer. This study investigates the antidiabetic potential Cassia bakeriana bark extracts fractions. We evaluate their ability to inhibit α‐amylase α‐glucosidase enzymes advanced glycation end‐products (AGEs). The antioxidant was also examined. Extracts were prepared through maceration with hexane (HE) ethanol (EE), fractions obtained via liquid–liquid extraction from EE. Anti‐enzymatic, anti‐glycation, antioxidant, cytotoxic assays conducted in 96‐well plates using different concentrations samples determine half‐maximal inhibitory concentration (IC 50 ). Active further analyzed HPLC‐(−)‐ESI‐MS/MS. ethyl acetate fraction (EAF) demonstrated high percentage inhibition (94.0%) promising IC value 1.05 μg mL −1 . Additionally, EAF displayed 61.5% α‐glucosidase, an 537 EE, EAF, n ‐butanol (BF) exhibited strong anti‐glycation capacities. Furthermore, BF, dichloromethane showed activity DPPH ORAC methodologies. Cytotoxic evaluated Vero cells, revealing no adverse effects on cell viability (CC > 512.0 predominantly comprised proanthocyanidins, flavonoids, anthraquinone, representing main constituents C. bark. provides first assessment comprehensive analysis chemical composition its active fractions, offering hope for future treatments.

Language: Английский

Antidiabetic, Genoprotective Potential, and Phytoconstituents of Cymodocea serrulata (R. Brown): In vivo, Drug Target Prediction, and ADME Studies DOI
Sahar S. El Souda, Usama W. Hawas, Lamia T. Abou El-Kassem

et al.

Thalassas An International Journal of Marine Sciences, Journal Year: 2025, Volume and Issue: 41(1)

Published: Feb. 1, 2025

Language: Английский

Citations

0
Food Phytochemicals as Epigenetic Modulators in Diabetes: A Systematic Review DOI Creative Commons
Fahrul Nurkolis, Nurpudji Astuti Taslim, Rony Abdi Syahputra

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101873 - 101873

Published: March 1, 2025

Language: Английский

Citations

0
The antidiabetic, haematological, and antioxidant implications of Schimpera arabica natural plant on Streptozotocin-diabetic rats DOI Creative Commons
Eman Ramadan Elsharkawy, Ali Robaian Alqahtani, Md. Nazim Uddin

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101891 - 101891

Published: April 1, 2025

Language: Английский

Citations

0
Phytochemical Profiling by HPLC‐ESI‐MS/MS and In Vitro Investigation of the Antidiabetic Activity of Cassia bakeriana Bark Extract and Fractions DOI Open Access
Diego Godina Prado, Allisson Benatti Justino,

Tiara da Costa Silva

et al.

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(12)

Published: Nov. 15, 2024

ABSTRACT Type 2 diabetes mellitus is a global health problem, placing patients at higher risk of developing cardiovascular diseases and cancer. This study investigates the antidiabetic potential Cassia bakeriana bark extracts fractions. We evaluate their ability to inhibit α‐amylase α‐glucosidase enzymes advanced glycation end‐products (AGEs). The antioxidant was also examined. Extracts were prepared through maceration with hexane (HE) ethanol (EE), fractions obtained via liquid–liquid extraction from EE. Anti‐enzymatic, anti‐glycation, antioxidant, cytotoxic assays conducted in 96‐well plates using different concentrations samples determine half‐maximal inhibitory concentration (IC 50 ). Active further analyzed HPLC‐(−)‐ESI‐MS/MS. ethyl acetate fraction (EAF) demonstrated high percentage inhibition (94.0%) promising IC value 1.05 μg mL −1 . Additionally, EAF displayed 61.5% α‐glucosidase, an 537 EE, EAF, n ‐butanol (BF) exhibited strong anti‐glycation capacities. Furthermore, BF, dichloromethane showed activity DPPH ORAC methodologies. Cytotoxic evaluated Vero cells, revealing no adverse effects on cell viability (CC > 512.0 predominantly comprised proanthocyanidins, flavonoids, anthraquinone, representing main constituents C. bark. provides first assessment comprehensive analysis chemical composition its active fractions, offering hope for future treatments.

Language: Английский

Citations

0