Global nutritional challenges and opportunities: Buckwheat, a potential bridge between nutrient deficiency and food security

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 145, P. 104365 - 104365

Published: Feb. 5, 2024

Language: Английский

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Systematic identification of wheat spike developmental regulators by integrated multi-omics, transcriptional network, GWAS, and genetic analyses

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(3), P. 438 - 459

Published: Feb. 2, 2024

Language: Английский

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Deciphering the Transcriptional Regulatory Network Governing Starch and Storage Protein Biosynthesis in Wheat for Breeding Improvement

Advanced Science, Journal Year: 2024, Volume and Issue: 11(33)

Published: June 28, 2024

Starch and seed storage protein (SSP) composition profoundly impact wheat grain yield quality. To unveil regulatory mechanisms governing their biosynthesis, transcriptome, epigenome profiling is conducted across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, H3K27me3 collectively regulate SSP starch genes with varying impact. Population transcriptome phenotype analyses highlight accessible promoter regions' crucial role as a genetic variation resource, influencing quality in core collection of accessions. Integration time-serial RNA-seq ATAC-seq enables the construction hierarchical transcriptional network identifying 42 high-confidence novel candidates. These candidates exhibit overlap regions associated size traits, functional significance validated through expression-phenotype association analysis among accessions loss-of-function mutants. Functional abscisic acid insensitive 3-A1 (TaABI3-A1) genome editing knock-out lines demonstrates its promoting accumulation while repressing biosynthesis regulation. Excellent TaABI3-A1

Language: Английский

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Decoding cellular transcriptional regulatory networks governing wheat inflorescence development

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

In wheat, inflorescence architecture critically determines yield potential, yet its structural complexity and asynchronous development have hindered cellular-resolution studies of spikelet floret formation. Here, we integrate spatial transcriptomics, high-sensitivity multiplexed error-robust fluorescence in situ hybridization (MERFISH), snRNA-seq across six developmental stages to generate a spatiotemporal atlas the wheat inflorescence. We identified 20 cell types, spatially resolved into three categories: 1) proliferating cells within spikelet, marked by active division; 2) supporting along central axis, including pith, cortex, vasculature; 3) developmental located both inside at base spikelets. The multi-omics approach enabled identification rare type ovary. Trajectory inference revealed that spikelets florets originate from two temporally distinct sub-clusters proliferating cells (R7), each defined high expression regulators. These findings challenge conventional model sequential meristem transitions (inflorescence-spikelet-floret) in wheat. Integration time-series snATAC-seq delineated cellular transcriptional regulatory networks (cTRNs) governing formation, mediated auxin cytokinin signaling, driven MADS-box transcription factors. Cell identity was maintained type-specific accessible chromatin regions (csACRs), which are enriched for SNPs associated with spike-related traits. For instance, csACRs WFZP DUO1 promoters affect TaNAC30 binding, regulating supernumerary phenotypes. Our work provides mechanistic framework identifies cTRN nodes as potential targets optimizing yield-related architecture.

Language: Английский

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Peculiarities of the biological potential of winter wheat (Triticum aestivum) under cultivation technologies of different intensity in the conditions of climate change

Regulatory Mechanisms in Biosystems, Journal Year: 2025, Volume and Issue: 33(1), P. e25009 - e25009

Published: Feb. 1, 2025

The importance of the wheat crop on a global scale for overcom ing problem hunger determines r e levance research increasing its productivity and sustainability in face climate change. Research realization genetic potential winter is an important step towards ensuring food security, improving agricultural efficiency preserving environment. ( Triticum aestivum L.) stands as pivotal element elevating productivity. Cultivation technologies varying intensities play crucial role achievement, they hold capacity to ensure high yields grain quality this crop. These are developed with considerations biological characteristics responses changing climatic conditions. presented here aimed investigate influence cultiv tion different unlocking wheat. Models cultivation were examined, which differed rates mineral fertilisers applied, insertion pea straw residues, application plant protection system. Through morpho-physiological investigations, fund mental patterns initiation florets within spike meristems central tiller stems established, along number (grains) depending phenological development stages plants cult i vation technology. impact technologiy stem density dynamics was optimal par meters determined, degree productive expression reduction magnitude assessed. Potential yield values calculated at main plants, their realiz level highest obtained under highly-intensive technology 1, P 80 K 100 N 240 residues preceding integrated protection. Morpho-physiological attributed such t o 770 stems/m 2 average ear 1.42 g. stage 65 36.4%, while 36.9%, 34.7% total retained from 32 92.

Language: Английский

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Unveiling the genetic Landscape of agronomic traits in bread wheat through Genome wide association Studies

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Critical understanding of the genetic basis yield-related traits through identification quantitative trait loci (QTLs) is essential for accelerating wheat improvement. The aim this study was to evaluate diverse genotypes and conducting GWAS pinpoint genomic regions responsible these traits. A field trial with 150 bread conducted eleven in RCBD design three replicates. Statistical analysis included Pearson’s correlation, step-wise multiple regression, structural equation modeling, principal component (PCA) were performed assess relationships. genome-wide association studies (GWAS) panel genotyped using a 37K SNP array identify associations. total 37,401 single nucleotide polymorphisms (SNPs) analyzed recognize 39 marker-trait associations (MTAs) across panel. Nine MTAs PH, 18 PL, one tillers, FLL, SL, GPS, biomass, GYPP identified. most important contributing yield spike weight, plant height, peduncle length tillers. Genotypic clustering germplasm revealed significant clusters among lines. This provides perceptive framework underlying key agronomic wheat. related those offers critical insights breeding programs aimed at improving yield. Future research should validate markers environments integrate selection Marker assisted resilient

Language: Английский

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Genomics for Yield and Yield Components in Durum Wheat

Plants, Journal Year: 2023, Volume and Issue: 12(13), P. 2571 - 2571

Published: July 7, 2023

In recent years, many efforts have been conducted to dissect the genetic basis of yield and components in durum wheat thanks linkage mapping genome-wide association studies. this review, starting from analysis bases that regulate expression for developing new varieties, we highlighted how, currently, reductionist approach, i.e., dissecting into its individual components, does not seem capable ensuring significant increases due diminishing resources, land loss, ongoing climate change. However, despite identification genes and/or chromosomal regions, controlling grain is still a challenge, mainly polyploidy level species. underline next-generation sequencing (NGS) technologies coupled with improved genome assembly high-throughput genotyping platforms, as well editing technology, will revolutionize plant breeding by providing great opportunity capture variation can be used programs. To date, genomic selection provides valuable tool modeling optimal allelic combinations across whole maximize phenotypic potential an under given environment.

Language: Английский

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Unraveling wheat endosperm development: epigenetic regulation and novel regulators for enhanced yield and quality

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Starch content and seed storage protein (SSP) composition are critical factors influencing wheat grain yield quality. To uncover the molecular mechanisms governing their biosynthesis, we conducted transcriptome epigenome profiling across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, H3K27me3 collectively regulate SSP starch genes with varying impact. Population phenotype analyses highlighted crucial role of accessible promoter regions as a genetic variation resource, quality in core collection accessions. By integrating time-serial RNA-seq ATAC-seq data, constructed hierarchical transcriptional regulatory network (TRN) identifying 42 high-confidence novel candidates. These candidates exhibited overlap associated size traits, functional significance was validated through expression-phenotype association analysis among accessions TILLING mutants. In-depth abscisic acid insensitive 3-A1 (TaABI3-A1) genome editing knock-out lines demonstrated its promoting accumulation while repressing biosynthesis regulation. An elite haplotype TaABI3-A1 higher weight identified during breeding process China, superior trait altered expression levels. Additionally, potential upstream regulator, GAGA-binding transcription factor 1 (TaGBP1), expression. Our study provides regulators, presenting an effective strategy for understanding regulation contributing to enhancement.

Language: Английский

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Comparison of population photosynthesis characteristics and grain yield of wheat under various sowing dates and seeding rates

Crop Science, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Abstract This study investigated the effects of sowing dates and seeding rates on photosynthetic characteristics grain yield in wheat ( Triticum aestivum L.). A field experiment was conducted with three dates—early date (SD‐E), normal (SD‐N), delay (SD‐D)—and rates—90 low rate (SR‐L), 108 moderate (SR‐M), high 126 kg ha −1 (SR‐H). The results revealed that at SR‐L SD‐E regime, SR‐M SD‐N SR‐H SD‐D regime increased by 7.48%–9.93%, 14.1%–16.7%, 19.0%–41.6%, respectively, compared to other combinations under same date. Consistent performance, aboveground biomass accumulation, nonstructural carbohydrates (NSC) accumulation stems, NSC remobilization amount from anthesis maturity were highest SD‐E, SD‐N, or among treatment combinations. Improved canopy (i.e., leaf area index, rate, photosynthetically active radiation interception use efficiency) also observed regime. Collectively, a suitable combined an optimum could enhance yield, increasing mitigate loss when delayed optimizing traits photosynthesis wheat.

Language: Английский

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Alleviative effect of iron chlorin e6 on isoproturon phytotoxicity to wheat

Weed Science, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: Oct. 9, 2024

Abstract Isoproturon phytotoxicity to wheat ( Triticum aestivum L.) is a worry for many farmers in chemical control of weeds fields, especially subzero weather conditions. Iron chlorin e6 (ICe6), new plant growth regulator, has been reported enhance crop stress resistance alleviate damage caused by stress; however, it not clear whether ICe6 an alleviative effect on isoproturon wheat. We determined the wheat, and 0.018 g ai ha −1 was optimal dose. Meanwhile, we also studied photosynthetic pigment content, parameters, oxidative indicators, antioxidant enzyme activity treated with three different treatments. found that activity, photosynthesis damaged were significantly lower than those control, hydrogen peroxide (H 2 O ) malondialdehyde (MDA) content increased. These results indicate weakened capacity The parameters (excluding intercellular CO concentration), isoproturon+ICe6– higher isoproturon-treated H MDA treatment maintained relatively improved capacity, allowing proceed normally. limits decrease effectively clearing excess reactive oxygen species ultimately alleviating membrane lipid peroxidation damage. In summary, only enhances increases yield crops such as soybean [ Glycine max (L.) Merr.] canola Brassica napus L.), but which manifested improvement abilities, leading increase shoot height fresh weight.

Language: Английский

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