Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)

ACS Omega, Journal Year: 2022, Volume and Issue: 7(36), P. 32153 - 32163

Published: Aug. 31, 2022

The widespread accumulation of nanoplastics is a growing concern for the environmental and human health. However, studies on mechanisms nanoplastic-induced developmental toxicity are still limited. Here, we systematically investigated potential biological roles nanoplastic exposure in zebrafish during early stage. embryos were subjected to 100 nm polystyrene with different concentrations (0, 100, 200, 400 mg/L). results indicated that could decrease hatching survival rates embryos. In addition, test exhibits via inhibition heart rate body length Besides, behavioral activity was also significantly suppressed after 96 h larvae. Further biochemical assays revealed activation oxidative stress responses, including reactive oxygen species enhanced superoxide dismutase catalase activities, might affect Furthermore, quantitative polymerase chain reaction assay demonstrated mRNA levels base excision repair (BER) pathway-related genes, lig1, lig3, polb, parp1, pold, fen1, nthl1, apex, xrcc1, ogg1, altered 24 exposure, indicating BER pathway would be stimulated Therefore, our findings illustrated induce through response pathways zebrafish.

Language: Английский

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Genotoxicity of Particles From Grinded Plastic Items in Caco-2 and HepG2 Cells

Frontiers in Public Health, Journal Year: 2022, Volume and Issue: 10

Published: July 6, 2022

Large plastic litters degrade in the environment to micro- and nanoplastics, which may then enter food chain lead human exposure by ingestion. The present study explored ways obtain nanoplastic particles from real-life containers. first set of experiments gave rise polypropylene suspensions with a hydrodynamic particle size range between 100 600 nm, whereas same grinding process polyethylene terephthalate (PET) produced primary 300 nm. did not cause cytotoxicity measured lactate dehydrogenase (LDH) water soluble tetrazolium 1 (WST-1) assays Caco-2 HepG2 cells. Nanoplastics transparent PET containers modest concentration-dependent increase DNA strand breaks, alkaline comet assay [net induction 0.28 lesions/106 bp at highest concentration (95% CI: 0.04; 0.51 base pair)]. nanoplastics was also positively associated breaks [i.e., net 0.10 pair -0.04; 0.23 pair)] concentration. black colored demonstrated no effect on cells terms cytotoxicity, reactive oxygen species production or changes cell cycle distribution. 0.43 0.09; 0.78 bp) Collectively, results indicate that consumer products can genotoxicity cultures.

Language: Английский

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Characterization of glyphosate-induced cardiovascular toxicity and apoptosis in zebrafish

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 851, P. 158308 - 158308

Published: Aug. 28, 2022

Language: Английский

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Nanoplastics and Microplastics May Be Damaging Our Livers

Toxics, Journal Year: 2022, Volume and Issue: 10(10), P. 586 - 586

Published: Oct. 4, 2022

Plastics in the environment can be degraded and even broken into pieces under action of natural factors, products with a particle size less than 5 mm are called microplastics (MPs). MPs exist variety environmental media that come contact human body. It enter body through food chains. At present, there many studies investigating damage to marine organisms mammals. The liver is largest metabolizing organ plays an important role metabolism However, no available systematic review on toxic effects liver. This paper summarizes adverse mechanisms liver, by searching literature highlighting have been published date, provides scenario for toxicity caused MPs.

Language: Английский

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Microscopic techniques as means for the determination of microplastics and nanoplastics in the aquatic environment: A concise review

Green Analytical Chemistry, Journal Year: 2022, Volume and Issue: 3, P. 100036 - 100036

Published: Oct. 1, 2022

The global concern about the fate of plastic particles, including microplastics (MPs) and nanoplastics (NPs), in aquatic ecosystems has undoubtedly grown as million tons polymeric fragments are released oceans an annual basis. ability these items being transferred into several combination with their to absorb organic or inorganic pollutants onto surface, raises need for a sufficient monitoring MPs/NPs complex environmental matrices. In frame these, microscopic techniques considered ideal tools critically assist other analytical due fast-screening character even complete detailed analysis particles some cases. light above, present review discusses group used isolated from samples most likely be important future actions, emphasizing on operating fundamentals, merits limitations. Moreover, highlighted green particles/fragments minimum reagents, solvents, energy required implementation such kind analysis. Finally, effective spectroscopic ones also presented.

Language: Английский

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