Low-dose thiram exposure elicits dysregulation of the gut microbial ecology in broiler chickens DOI Creative Commons
Lei Wang, Fazul Nabi,

Weixue Yi

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 270, P. 115879 - 115879

Published: Dec. 28, 2023

Thiram, a typical fungicide pesticide, is widely used in agricultural production. The presence of thiram residues not only due to over-utilization, but also primarily attributed long-term accumulation. However, there paucity information regarding the impact prolonged utilization at low doses on gut microbiota, particularly with respect fungi. Our objective explore effect broilers from perspective which includes both bacteria and We developed low-dose model simulate residue employed 16 S rRNA ITS gene sequencing investigate diversity profile microbiota between group CC (normal diet) TC diet supplemented 5 mg/kg thiram). results revealed that had detrimental broiler's growth performance, resulting an approximate reduction 669.33 g their final body weight day 45. findings indicated negative bacterial composition, leading notable abundance Merdibacter, Paenibacillus, Macrococcus, Fournierella, Anaeroplasma (p < 0.05) compared group. Conversely, relative level Myroides was significantly increased response exposure. In fungi, enhanced richness fungal populations 0.05), as evidenced by increase alpha indices, i.e. ACE (CC: 346.49 ± 117.27 vs TC: 787.27 379.14, p Chao 1 317.63 69.13 504.85 104.50, Shannon 1.28 1.19 5.39 2.66, Simpson 0.21 0.78 0.34, 0.05). Furthermore, Ascomycota, Kickxellomycota, Glomeromycota were exposure thiram, conversely, Basidiomycota decreased Overall, this study demonstrated induced significant changes composition broilers, more pronounced observed community community. Importantly, our further emphasize potential risks associated dose have novel discovery indicating alterations fungi may serve crucial factor contributing effects exerted residues.

Language: Английский

The effect of miR-205a with RUNX2 towards proliferation and differentiation of chicken chondrocytes in thiram-induced tibial dyschondroplasia DOI Creative Commons
Yuxin Zhou, Yuxiang Lu, Hengyong Xu

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104535 - 104535

Published: Nov. 8, 2024

Tibial dyschondroplasia (TD) is a kind of metabolic bone disease in fast-growing broilers, which seriously restricts the development poultry industry. Our previous studies have revealed significant upregulation miR-205a TD cartilage tissue, suggesting its potential role as regulatory factor pathogenesis TD. However, precise function implications and underlying mechanism remain elusive. Therefore, this study aims to elucidate biological functions mechanisms progression by employing mehtodologies such qRT-PCR, CCK-8 assay, EdU assays, flow cytometry. The findings demonstrated that transfection overexpression plasmid reduced chondrocytes growth while enhancing apoptosis; conversely, blocking had opposite effects. RUNX2 was identified target gene through biosynthesis dual luciferase helps grow develop. when both were overexpressed, effect significantly suppressed. In conclusion, plays slowing targeting reducing expression, initiate progress

Language: Английский

Citations

0
Rapid detection of thiram on apple surfaces using a flexible and sticky SERS substrate coupled with chemometric methods DOI

Sasa Peng,

Zhilong Zhang, Jialin Guo

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 328, P. 125435 - 125435

Published: Nov. 19, 2024

Language: Английский

Citations

0
Low-dose thiram exposure elicits dysregulation of the gut microbial ecology in broiler chickens DOI Creative Commons
Lei Wang, Fazul Nabi,

Weixue Yi

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 270, P. 115879 - 115879

Published: Dec. 28, 2023

Thiram, a typical fungicide pesticide, is widely used in agricultural production. The presence of thiram residues not only due to over-utilization, but also primarily attributed long-term accumulation. However, there paucity information regarding the impact prolonged utilization at low doses on gut microbiota, particularly with respect fungi. Our objective explore effect broilers from perspective which includes both bacteria and We developed low-dose model simulate residue employed 16 S rRNA ITS gene sequencing investigate diversity profile microbiota between group CC (normal diet) TC diet supplemented 5 mg/kg thiram). results revealed that had detrimental broiler's growth performance, resulting an approximate reduction 669.33 g their final body weight day 45. findings indicated negative bacterial composition, leading notable abundance Merdibacter, Paenibacillus, Macrococcus, Fournierella, Anaeroplasma (p < 0.05) compared group. Conversely, relative level Myroides was significantly increased response exposure. In fungi, enhanced richness fungal populations 0.05), as evidenced by increase alpha indices, i.e. ACE (CC: 346.49 ± 117.27 vs TC: 787.27 379.14, p Chao 1 317.63 69.13 504.85 104.50, Shannon 1.28 1.19 5.39 2.66, Simpson 0.21 0.78 0.34, 0.05). Furthermore, Ascomycota, Kickxellomycota, Glomeromycota were exposure thiram, conversely, Basidiomycota decreased Overall, this study demonstrated induced significant changes composition broilers, more pronounced observed community community. Importantly, our further emphasize potential risks associated dose have novel discovery indicating alterations fungi may serve crucial factor contributing effects exerted residues.

Language: Английский

Citations

0