Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Acute diarrhea and cholera (AWD/C) result in more than 21000 to 143000 global mortality annually are associated with Vibrio cholerae. The pathogen has shown increasing evolutionary/emerging dynamics linked mobilome or ubiquitous nature of mobile integrative genetic conjugative elements (MIGCE), however, such rarely reported amongst somatic-antigen non-agglutinating Type-1/-139 V. cholerae (SA-NAG-T-1/139Vc). study reports the detection mobilome-associated indices SA-NAG-T-1/139Vc as a potential strategy for differentiating/discriminating emerging environmental bacteria. Presumptive isolates were retrieved from five water sources, while strains characterized/serogrouped confirmed using simplex comparative-genomic-multiplex Polymerase Chain Reaction (PCR). Genomic island (GI-12det, GI-14det, GI-15det); Phages (TLC-phagedet, Kappa-phagedet) ICEs SXT/R391 family genes (SXT/R391-ICEs integrase, SXT-Hotspot-IV, ICEVchInd5Hotspot-IV, ICEVchMoz10Hotspot-IV) detected. Other rare ICE members ICEVcBan8att gene Seventh Pandemic (VSP-II Integrase, Prototypical VSP-II) also Results revealed that 8.22% (61/742) serogroup observed harbors integrase (34/61; 55.7%) other traits including; attB/attP (29/61; 47.5%, 14/61; 23%), (4/61; 6.56%), phage types (TLC-phagedet: 2/61; 3.28% Kappa-phagedet: 7/61; 11.48%) well (INT1, Sul1, Sul2) (29/61: 47.5%; 21/61: 34.4%; 25/61: 41%). Such determinants/MIGCE suggests discriminatory tendencies SA-NAG-T-1/139Vcwhich may be applied typing evolving/emerging need encourage application continuous these is suggestive interest controlling future strains.

Language: Английский