Understanding
the
inter-relationships
between
actual
microbial
communities
and
various
petroleum
hydrocarbon
components
remains
a
key
challenge
for
effective
efficient
decontamination
in
oilfield
soil.
Dynamic
changes
of
reconstructed
PIONA
fractions
community
structures
were
investigated.
The
results
showed
that
biodegradation
occurred
on
day
15
bioaugmentation,
efficiency
paraffin
reached
96.5%
after
60
days
1%
oil-contaminated
strain
M3
promoted
enrichment
Balneolaceae,
Halolalama,
Woeserchaeia,
delayed
colonization
Alcanivorax.
Weighted
gene
co-expression
network
analysis
(WGCNA)
positive
interactions
different
microbes
7
modules
more
significant
than
negative
interactions,
revealed
synergistic
degradation
constituents.
abundance
alcohol
dehydrogenase,
aldehyde
catechol
cleavage
enzyme,
cytochrome
P450,
gentisate
dioxygenase,
benzoate/toluate
dioxygenase
increased
abruptly
initial
with
inoculation
M3.
This
study
provides
insights
into
inner
mechanisms
metabolism
under
combined
action
exogenous
indigenous
ACS ES&T Engineering,
Journal Year:
2023,
Volume and Issue:
3(11), P. 1758 - 1769
Published: Aug. 1, 2023
Microbially
driven
aggregate
formation
plays
a
sustainable
role
during
the
soil
process
in
bauxite
residue,
which
may
realize
large-scale
treatment
of
typical
alkaline
solid
waste.
However,
potential
mechanisms
microbial
metabolites
on
organic–alkaline
mineral
complexes
and
stable
aggregates
are
usually
neglected.
Here,
we
developed
novel
microbially
strategy
to
improve
enhance
ecological
service
resilience
by
inoculation
Penicillium
oxalicum
(P.
oxalicum,
an
alkali-tolerant
acid-producing
fungi).
The
intensive
application
P.
reduced
contents
electrical
conductivity
(EC)
exchangeable
sodium
percentage
(ESP)
while
increasing
proportions
macroaggregates
microaggregates
residue
compared
chemical
amendments.
Furthermore,
even
inoculated
with
same
dose
multiple
inoculations
achieved
higher
extracellular
metabolic
activity
than
single
inoculation.
Simultaneously,
guided
oligotrophic-dominated
assemblages
(including
Actinobacteria,
Proteobacteria,
Ascomycota
members)
increased
network
complexity.
spectroscopic
analysis
demonstrated
that
polymeric
substances
(EPSs)
promoted
due
high
adsorptive
functional
groups
(e.g.,
−OH,
−COOH,
N–H).
existence
hydrogen
bonds
led
viscosity
exhibition
through
binding
effects
polysaccharides
minerals
sodalite,
cancrinite).
findings
enhanced
understanding
EPSs
stabilization
rehabilitation
residue.
