UTTAR PRADESH JOURNAL OF ZOOLOGY,
Journal Year:
2023,
Volume and Issue:
44(23), P. 46 - 51
Published: Nov. 17, 2023
The
acute
toxicity
evaluation
of
cypermethrin,
a
pyrethroid
to
common
carp
i.e.
Cyprinus
carpio
was
done
with
an
emphasis
on
histopathological
effects.
In
this
investigation,
fish
were
exposed
different
concentrations
Cypermethrin
(25%
EC)
for
21
days.
C.
treated
sublethal
(5,
10,
15,
and
20
percent
96
hr
LC50
value
2.924
µl/L)
cypermethrin
three
exposure
periods,
7,
14,
Control
sacrificed
at
interval
7
days
the
vital
tissues
like
brain,
gills,
kidney
taken
analyze
alterations.
During
experimental
period,
respiratory
stress
erratic
swimming
activities,
disrupted
equilibrium,
response
observed
which
continuously
varied
increasing
toxicant
concentration.
proliferation
cells
filament,
inflammation,
hemorrhage,
epithelial
cell
destruction,
fusion
secondary
lamellae,
necrosis
noticed.
Hence
it
is
concluded
that
poses
serious
threat
aquatic
fauna
ecosystem
can
be
assessed
via
histological
studies.
Environmental Science & Technology,
Journal Year:
2023,
Volume and Issue:
58(1), P. 160 - 170
Published: Dec. 26, 2023
Pesticides
and
antibiotics
are
believed
to
increase
the
incidence
of
antibiotic
resistance
genes
(ARGs)
virulence
factor
(VFGs),
constituting
a
serious
threat
global
health.
However,
impact
this
combined
pollution
on
microbiome
that
related
ARGs
VFGs
soil–plant–animal
systems
remain
unknown.
In
study,
60-day
microcosm
experiment
was
conducted
reveal
effects
zinc
thiazole
(ZT)
oxytetracycline
(OTC)
microbial
communities,
resistomes,
factors
in
soil,
earthworm
gut,
phyllosphere
samples
using
metagenomics.
ZT
exposure
perturbed
communities
nutrient
metabolism
increased
abundance
gut.
Combined
changed
profiles
by
decreasing
diversity
phyllosphere.
Host-tracking
analysis
identified
some
genera,
such
as
Citrobacter
Aeromonas,
frequent
hosts
Notably,
co-occurrence
patterns
MGEs
were
observed
metagenome-assembled
contigs.
More
importantly,
markedly
potentially
drug-resistant
pathogens
Acinetobacter
soli
junii
Overall,
study
expands
our
current
understanding
spread
under
pollutant-induced
stress
associated
health
risks.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(10), P. 4476 - 4486
Published: Feb. 21, 2024
Antibiotic
resistance
genes
(ARGs)
are
ancient
but
have
become
a
modern
critical
threat
to
health.
Gut
microbiota,
dynamic
reservoir
for
ARGs,
transfer
between
individuals.
Surveillance
of
the
antibiotic
resistome
in
gut
during
different
host
growth
phases
is
understanding
dynamics
this
ecosystem.
Herein,
we
disentangled
ARG
profiles
and
mechanism
ARGs
egg
adult
Tetramorium
caespitum.
Experimental
results
showed
remarkable
difference
both
microbiota
with
development
T.
Meta-based
metagenomic
indicated
generalizability
development.
By
using
Raman
spectroscopy
metabolomics,
metabolic
phenotype
metabolites
that
biotic
phase
significantly
changed
lipid
metabolism
as
caespitum
aged.
Lipid
were
demonstrated
main
factor
driving
enrichment
Cuminaldehyde,
antibacterial
metabolite
displayed
increase
phase,
was
strongly
induce
abundance.
Our
findings
show
developmental
stage-dependent
likely
modulated
by
metabolites,
offering
novel
insights
into
possible
steps
reduce
dissemination
soil
food
chain.
Bacterial
antibiotic
resistance
has
recently
attracted
increasing
amounts
of
attention.
Here,
an
artificially
antibiotic-resistant
bacterial
community
(ARBC)
combined
with
five
different
constructed
bacteria
(ARB)
single
resistance,
namely,
kanamycin
(KAN),
tetracycline
(TET),
cefotaxime
(CTX),
polymyxin
B
(PB),
or
gentamicin
(GEM),
was
studied
for
the
stress
response
to
photocatalysis.
With
photocatalytic
inactivation,
transfer
and
diffusion
genes
(ARGs)
in
ARBC
decreased,
fewer
multidrug-resistant
(MDRB)
emerged
aquatic
environments.
After
several
days
inactivation
Luria
broth
cultivation,
>90%
ARB
were
transformed
antibiotic-susceptible
by
discarding
ARGs.
Bacteria
double
dominant
species
(99%)
residual
ARB.
The
changes
ARG
abundance
varied,
decreasing
GEM
TET
KAN
genes.
change
level
consistent
abundance.
Correspondingly,
point
mutations
occurred
KAN,
CTX
PB
after
which
might
be
reason
why
these
persisted
longer
ARBC.
In
summary,
could
reduce
some
ARGs
inhibit
emergence
MDRB
as
well
block
This
work
highlights
advantages
long-term
controlling
facilitates
a
better
understanding
communities
real
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(6), P. 2931 - 2943
Published: Feb. 2, 2024
From
a
"One
Health"
perspective,
the
global
threat
of
antibiotic
resistance
genes
(ARGs)
is
associated
with
modern
agriculture
practices
including
agrochemicals
application.
Chiral
fungicides
account
for
considerable
proportion
wildly
used
agrochemicals;
however,
whether
and
how
their
enantiomers
lead
to
differential
proliferation
in
agricultural
environments
remain
overlooked.
Focused
on
soil-earthworm
ecosystem,
we
first
time
deciphered
mechanisms
underlying
enantioselective
driven
by
typical
chiral
fungicide
mandipropamid
(i.e.,
R-MDP
S-MDP)
utilizing
multiomic
approach.
Time-series
metagenomic
analysis
revealed
that
led
significant
enhancement
ARGs
potential
mobility
(particularly
plasmid-borne
ARGs)
earthworm
intestinal
microbiome.
We
further
demonstrated
induced
concentration-dependent
facilitation
plasmid-mediated
ARG
transfer
among
microbes.
In
addition,
transcriptomic
verification
identified
key
aspects
involved,
where
enhanced
cell
membrane
permeability,
ability,
biofilm
formation
quorum
sensing,
rebalanced
energy
production,
decreased
versus
S-MDP.
Overall,
findings
provide
novel
insights
into
disruption
microbiome
resistome
gut
offer
contributions
comprehensive
risk
assessment
agroecosystems.
While
substantial
amounts
of
antibiotics
and
pesticides
are
applied
to
maintain
orchard
yields,
their
influence
on
the
dissemination
risk
antibiotic
resisitome
in
food
chain
remains
poorly
understood.
In
this
study,
we
characterized
bacterial
fungal
communities
differentiated
both
resistance
genes
(ARGs)
virulence
factor
(VFGs)
soil,
Chinese
bayberry
(matured
fallen),
fruit
fly
gut,
collected
from
five
geographic
locations.
Our
results
showed
that
guts
soils
exhibit
a
higher
abundance
ARGs
VFGs
compared
with
fruits.
We
identified
112
shared
75
VFGs,
aminoglycoside
adherence
being
among
most
abundant.
The
co-occurrence
network
revealed
some
microbes,
such
as
Human
activity
is
accelerating
the
emergence
of
fungal
pathogens,
prompting
substantial
efforts
to
discover
novel
fungicides.
The
runoff
and
spray
drift
from
agricultural
fields
adversely
affect
aquatic
terrestrial
nontarget
organisms.
However,
few
studies
have
examined
effects
co-contamination
by
agrochemical
fungicides
pharmaceutical
antibiotics
on
microorganisms
antibiotic
resistance
genes
(ARGs)
in
soil-animal-plant
system.
To
further
explore
mechanisms,
we
investigated
individual
combined
a
widely
used
fungicide
(cyazofamid,
CZF)
last-resort
(colistin,
polymyxin
E,
PME)
soil-earthworm-tomato
Our
study
revealed
that
CZF
PME
exerted
synergistic
toxicity,
significantly
reducing
earthworm
survival
inhibiting
tomato
growth.
microbial
community
structure
was
perturbed,
specific
bacteria
were
enriched.
Fungicides
had
distinct
bacterial
functional
pathways:
CZF+PME
treatments
enhanced
compound
degradation,
whereas
promoted
biological
nitrogen
cycling.
Moreover,
increased
abundance
insertional
plasmid-associated
number
totalARGs
bulk
rhizosphere
soil.
We
also
linkages
between
communities
resistome.
findings
provide
new
insights
into
potential
impacts
complex
real-life
environments,
such
as
soil–animal–plant
systems.
