Response of TCE Biodegradation to Elevated H2 and O2: Implication for Electrokinetic-Enhanced Bioremediation DOI

Weiwei Ouyang,

Yao Huang, Li Cui

et al.

Published: Jan. 1, 2023

Electrokinetic-enhanced bioremediation (EK-Bio) has been developed as a promising technology for the remediation of subsurface trichloroethylene (TCE) contamination. The effects produced pure and abundant H2 O2 by anode cathode EK-Bio on microbial community changes TCE biodegradation remain unknown. This study investigated influence high dissolved (DH, 0.2-0.7 mg/L) (DO, 2.6-6.6 concentrations transformation indigenous communities in TCE-contaminated sediment, including TCE-removing populations, using 16S rRNA sequencing, functional gene analyses. results showed that introduction enhanced degradation, causing 90.4% removal four weeks, cis-dichloroethylene (cis-DCE) was accordingly. following three ways should be responsible this promoted degradation. Firstly, DH rapidly reduced oxidation-reduction potential (ORP) value to around -500 mV, beneficial dechlorination. Secondly, significantly changed enrichment anaerobic dechlorinators, such Sulfuricurvum, Sulfurospirillum, Geobacter, Desulfitobacterium, increased abundance dechlorination pceA. Thirdly, environment alleviated electronic competition between dechlorinators sulfate-reducing bacteria. However, bio-remediation did not occur DO even after ten weeks due decreases aerobic co-metabolic microbes Pseudomonas Comamonas shortage growth substrate mmoX carrier Methyloversatilis. competitive organic carbon (DOC) consumption unfriendly microbe-microbe interactions also interpreted non-degradation environment. These provided evidence mechanism EK-Bio. Providing obligate metabolic bacteria electrochemical cathodes anodes, respectively, or substrates can feasible methods promote shallow aquifer under technology.

Language: Английский

Degradation of 1,2-dichloroethane in real polluted groundwater by using enriched bacterial consortia in aerobic and anaerobic laboratory-scale conditions DOI
Federica De Marines, Ilenia Cruciata, Gaetano Di Bella

et al.

International Biodeterioration & Biodegradation, Journal Year: 2023, Volume and Issue: 183, P. 105644 - 105644

Published: July 1, 2023

Language: Английский

Citations

10
Integrating Enzyme-Based Kinetics in Reactive Transport Models to Simulate Spatiotemporal Dynamics of Biomarkers during Chlorinated Ethene Degradation DOI Creative Commons
Diego Di Curzio, Michele Laureni, Mette Martina Broholm

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(46), P. 20642 - 20653

Published: Nov. 7, 2024

Biomarkers such as functional gene mRNA (transcripts) and proteins (enzymes) provide direct proof of metabolic regulation during the reductive dechlorination (RD) chlorinated ethenes (CEs). Yet, current models to simulate their spatiotemporal variability are not flexible enough mimic homologous behavior RDase genes. To this end, we developed new enzyme-based kinetics model concentrations CEs together with transcript enzyme levels RD. First, was calibrated existing microcosm data on RD cis-DCE. The mirrored tceA vcrA expression production enzymes in Dehalococcoides spp. Considering instead nonhomologous improved fitting time series. Second, biomarker patterns were explored a concept under groundwater flow conditions, considering degraders occurring immobile mobile states. Under both biomarker-rate relationships nonlinear hysteretic because acted biomarkers additionally undergo advective-dispersive transport, which increases nonlinearity makes observed even more challenging interpret. offers thorough mechanistic description while also allowing simulation dynamic various key aquifers.

Language: Английский

Citations

3
Molasses-based in situ bio-sequestration of Cr(VI) in groundwater under flow condition DOI
Tianyuan Gu, Wen-jing Niu, Lili Huo

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 344, P. 123337 - 123337

Published: Jan. 22, 2024

Language: Английский

Citations

2
Biochar, microbes, and biochar-microbe synergistic treatment of chlorinated hydrocarbons in groundwater: a review DOI Creative Commons

Shixin Niu,

Changsuo Li,

Shuai Gao

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 18, 2024

Dehalogenating bacteria are still deficient when targeted to deal with chlorinated hydrocarbons (CHCs) contamination: e.g., slow metabolic rates, limited substrate range, formation of toxic intermediates. To enhance its dechlorination capacity, biochar and composites appropriate surface activity biocompatibility selected for coupled dechlorination. Because special physical chemical properties, it promotes biofilm by dehalogenating on improves the living environment bacteria. Next, provide active sites removal CHCs through adsorption, activation catalysis. These can be specific metal centers, functional groups or structural defects. Under microbial mediation, these undergo catalytic cycles, thereby increasing efficiency. However, there is a lack systematic understanding mechanisms in biogenic abiogenic systems based biochar. Therefore, this article comprehensively summarizes recent research progress as “Taiwan balm” degradation terms catalysis, improvement community structure promotion metabolism CHCs. The efficiency, influencing factors reaction mechanism degraded were also discussed. following conclusions drawn, pure system, fixed adsorption bonds surface; composite material relies persistent free radicals electron shuttle react CHCs, disrupting their molecular reducing them; biochar-coupled microorganisms reduce primarily forming an “electron bridge” between biological non-biological organisms. Finally, experimental directions carried out future suggested explore optimal solution improve treatment efficiency water.

Language: Английский

Citations

2
Biogenic amorphous FeOOH activated additional intracellular electron flow pathways for accelerating reductive dechlorination of tetrachloroethylene DOI
Yang Yu, Ang Li, Sheng-Qiang Fan

et al.

Water Research, Journal Year: 2024, Volume and Issue: 267, P. 122489 - 122489

Published: Sept. 20, 2024

Language: Английский

Citations

2
Microbiome resistance mediates stimulation of reduced graphene oxide to simultaneous abatement of 2,2′,4,4′,5-pentabromodiphenyl ether and 3,4-dichloroaniline in paddy soils DOI
Yi Sun, Ying Teng, Ran Li

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133121 - 133121

Published: Nov. 30, 2023

Language: Английский

Citations

4
Sustainable Abiotic–Biotic Dechlorination of Perchloroethene with Sulfidated Nanoscale Zero-Valent Iron as Electron Donor Source DOI

Junhong Wu,

Yin Zhong, Yirong Deng

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(47), P. 20931 - 20941

Published: Oct. 29, 2024

Combining organohalide-respiring bacteria with nanoscale zero-valent iron (nZVI) represents a promising approach for remediating chloroethene-contaminated aquifers. However, limited information is available regarding their synergistic dechlorinating ability chloroethenes when nZVI sulfidated (S-nZVI) under the organic electron donor–limited conditions typically found in deep Herein, we developed combined system utilizing mixed culture containing Dehalococcoides (Dhc) and S-nZVI particles, which achieved sustainable dechlorination repeated rounds of spiking 110 μM perchloroethene (PCE). The relative abundance Dhc considerably increased from 5.2 to 91.5% after five PCE, as evidenced by 16S rRNA gene amplicon sequencing. corrosion generated hydrogen an donor other volatile fatty acid (VFA)-producing bacteria. Electron balance analysis indicated that 68.1% electrons Fe0 consumed were involved dechlorination, 6.2, 1.1, 3.2% stored formate, acetate, VFAs, respectively. produced acetate possibly served carbon source Dhc. Metagenomic revealed Desulfovibrio, Syntrophomonas, Clostridium, Mesotoga likely VFA production. These findings provide valuable insights into mechanisms biotic abiotic important implications remediation aquifers contaminated chloroethenes.

Language: Английский

Citations

1
Enhanced thyroxine removal from micro-polluted drinking water resources in a bio-electrochemical reactor amended with TiO2@GAC particles: Efficiency, mechanism and energy consumption DOI
Weifang Ma,

Jiangru Lian,

Eldon R. Rene

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 237, P. 116949 - 116949

Published: Aug. 23, 2023

Language: Английский

Citations

3
Biodegradation of Bioplastic Polycaprolactone by Marine Bacterium Alteromonas Sp. Ghpt-2 DOI
Guangfeng Kan, He Lyu, Xiaofei Wang

et al.

Published: Jan. 1, 2024

Biodegradable plastics are developed and applied during the past thirty years, their biodegradation has drawn much attention. In present study, marine bacterium Alteromonas sp. ghpt-2 was identified to degrade polycaprolactone (PCL) effectively. The optimal values of degradation parameters were found at temperature 15 °C, pH 8.0, carbon source maltose NaCl concentration 0-3%, respectively. alteration PCL film represented after bacterial by differential scanning calorimetry, Fourier transform infrared spectroscopy electron microscopy. After incubation with PCL, metabolic pathways strain down-regulated including ribosome, TCA cycle, glyoxylate dicarboxylate metabolism, pyruvate oxidative phosphorylation, thermogenesis, flagella synthesis MAPK signal pathway, which affected cell growth multiplication. contrast, upregulated nitrogen metabolism ABC transporters pathway relieved stress maintained growth. By analysis, process proposed biofilm formation, biodeterioration assimilation. this a low tolerant degrading obtained that could be in treatment plastic waste, adversity cold high salinity.

Language: Английский

Citations

0
Leveraging weak electrical stimulation and artificial intelligence for sustainable microbial dehalogenation in groundwater remediation DOI
Miao Lv,

Qianjing Yao,

Zemin Qin

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 475 - 490

Published: Jan. 1, 2024

Language: Английский

Citations

0