Metabolites,
Journal Year:
2024,
Volume and Issue:
14(8), P. 454 - 454
Published: Aug. 17, 2024
The
increasing
use
of
chemicals
requires
a
better
understanding
their
presence
and
dynamics
in
the
environment,
as
well
impact
on
ecosystems.
aim
this
study
was
to
validate
first
steps
an
innovative
multi-omics
approach
based
metabolomics
16S
metabarcoding
data
for
analyses
fate
contaminants
Mediterranean
lagoons.
Semi-targeted
analytical
procedures
water
sediment
matrices
were
implemented
assess
chemical
contamination
lagoon:
forty-six
compounds
detected,
28
which
could
be
quantified
(between
0.09
47.4
ng/L)
0.008
26.3
ng/g)
samples
using
UHPLC-MS/MS
instrument.
In
addition,
non-targeted
(UHPLC-HRMS)
four
different
sample
preparation
protocols
solid/liquid
extractions
or
automated
pressurized
fluid
extraction
system
(EDGE®)
carried
out
determine
protocol
with
best
metabolome
coverage,
efficiency
reproducibility.
Solid/liquid
solvent
mixture
acetonitrile/methanol
(50/50)
evaluated
protocol.
Microbial
diversity
lagoon
also
measured
after
DNA
five
commercial
kits.
Our
showed
that
DNeasy
PowerSoil
Pro
Qiagen
kit
(Promega,
USA)
most
suitable
assessing
microbial
fresh
sediment.
