Environmental Technology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 15
Published: March 27, 2025
Heavy
metals
(HMs)
significantly
affect
the
anaerobic
fermentation
of
coal
slime
(CS),
while
sawdust
serves
as
a
promising
substrate
for
methane
bioconversion
and
an
effective
adsorbent
HMs.
To
explore
migration
HMs
improve
conversion
efficiency
CS
to
biomethane,
experiments
were
conducted
on
co-fermentation
with
different
mass
ratios.
FT-IR,
ICP-MS,
SEM-EDS,
metagenomic
sequencing
employed
elucidate
regulation
mechanism
microorganisms
after
adding
in
altering
toxicological
environment.
The
results
revealed
that
optimum
ratio
was
2:1.
Oxygen-containing
functional
groups
removed,
fatty
structure
degraded.
Addition
promoted
further
degradation
various
Metal
elements
such
Fe,
Mg,
Co,
Cd
had
also
been
detected
its
surface.
Bacillota
(39.05%)
enriched,
which
effectively
improved
microbial
community
structure.
abundance
species
metal
detoxification
functions
increased.
types
transporters
related
toxic
ions
increased
families
ATP-binding
Cassette
Superfamily
(ABC)
Resistance-Nodulation-Cell
Division
(RND),
would
aid
cellular
homeostasis.
Moreover,
addition
expression
resistance
genes
(MRGs).
Multi-MRGs
primarily
linked
Cu,
As,
Zn,
bacterial
contributing
key
MRGs
copA,
ziaA,
actP.
These
findings
offer
valuable
insights
support
clean
efficient
utilization
sawdust.
