Multi-omics insights into the response ofAspergillus parasiticusto long-chain alkanes in relation to polyethylene degradation DOI Creative Commons
Romanos Siaperas, George Taxeidis, Efstratios Nikolaivits

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Plastic pollution presents a global challenge, with polyethylene (PE) being among the most persistent plastics due to its durability and environmental resilience. In this study, we employed multi-omics approach study ability of Aspergillus parasiticus MM36, an isolate derived from Tenebrio molitor intestines, metabolize long-chain alkanes (lcAlk) secrete enzymes able modify PE. The fungus was grown hexadecane (C16) or mixture lcAlk (C24 C36) as carbon sources culture supernatants were tested daily for their Proteomic analysis identified induced oxidases potentially involved in PE functionalization. Key include multicopper oxidases, peroxidases, unspecific peroxygenase FAD-dependent monooxygenases. Surfactant proteins facilitating enzymatic cellular interaction hydrophobic lcAlk, such one hydrophobin, three surface-binding (HsbA) cerato platanin, present all secretomes. Transcriptomic comparing C16 cultures highlighted enrichment oxidoreductase activities carboxylic acid metabolism both incubation days, transmembrane transporters transferases predominating on day 2 biosynthetic processes 3. cultures, hydrolytic enzymes, including esterases, upregulated alongside Baeyer-Villiger monooxygenases, suggesting shift toward sub-terminal hydroxylation. Integrating transcriptomic secretomic data, propose mechanism assimilation by A. involving extracellular oxyfunctionalization, hydrocarbon uptake via surface-modifying channeling through membrane energy consumption processes. This provides insights into fungal mechanisms alkane highlights relevance plastic degradation. Importance challenge marine life human health, Hydroxylation is regarded initial step degradation, similar oxidation, making alkane-degrading microbes promising source degraders. used investigate MM36 that secretomes key biosurfactants enable interact transform substrates like further revealed biological metabolism. By integrating these insights, highlight biodegradation. work advances our understanding contributions addressing pollution.

Language: Английский

Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes DOI Creative Commons
Anton A. Stepnov, Esteban Lopez-Tavera, Ross R. Klauer

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 1, 2024

Language: Английский

Citations

11
Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere DOI Creative Commons
Muthukrishnan Sathiyabama,

R. V. Boomija,

Thrmiga Sathiyamoorthy

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 9, 2024

Plastic accumulation is a severe threat to the environment due its resistivity thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal interest because eco-friendly nature. this study, total 33 fungi were isolated from plastisphere out which 28 fungal species showed halo zone clearance in agarized LDPE media. The fungus showing highest was further used evaluate potential. Based on morphological molecular technique, identified as Cladosporium sphaerospermum. biodegradation by C. sphaerospermum evaluated various methods. exposure with resulted weight loss (15.23%) seven days, higher reduction rate (0.0224/day) lower half-life (30.93 days). FTIR analysis changes functional group increased carbonyl index treated SEMimages evidenced formation pits, surface aberrations grooves film whereas untreated control no change. AFM confirmed roughness film. This might be extracellular lignolytic enzymes secreted grown LDPE. polyethylene Short chain alkanes such dodecane, hexasiloxane silane extract incubated through GC-MS first report very short duration enables green scavenging wastes.

Language: Английский

Citations

8
Biodegradation of synthetic plastics by the extracellular lipase of Aspergillus niger DOI Creative Commons
Ayesha Safdar, Fatima Ismail, Muhammad Imran

et al.

Environmental Advances, Journal Year: 2024, Volume and Issue: 17, P. 100563 - 100563

Published: June 29, 2024

A rapid increase in plastic pollution is a major threat to the environment. One intriguing group of enzymes that can act as biocatalysts for breakdown polymers lipase. This study reports production lipase from Aspergillus niger MG654699.1 utilizing agro-industrial residue (wheat bran) through solid-state fermentation. The produced showed 176.55 U/mL activity, 7.18 mg/mL protein content, and 24.60 U/mg specific activity under optimal conditions 37°C pH 7.0. biocatalytic 30 KDa resulted 3.8%, 3.6%, 5% weight loss PE (polyethylene), PET (polyethylene terephthalate), PS (polystyrene), respectively. Application Fourier transform infrared spectroscopy (FTIR) scanning electron microscopy (SEM) confirmed lipase-mediated deterioration treated polymer samples. alterations functional groups surface structures samples chemical physical impact applied enzyme. findings this be employed an eco-friendly green biocatalyst effective depolymerization environmental waste.

Language: Английский

Citations

7
Exploring the potential of insect gut symbionts for polyethylene biodegradation DOI
Sameh S. Ali, Tamer Elsamahy,

Shimaa El-Sapagh

et al.

Process Safety and Environmental Protection, Journal Year: 2024, Volume and Issue: 190, P. 22 - 33

Published: Aug. 5, 2024

Language: Английский

Citations

6
Recent trends in microbial and enzymatic plastic degradation: a solution for plastic pollution predicaments DOI Creative Commons

Swagata Lakshmi Dhali,

Dinesh Parida, Bikash Kumar

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Aug. 11, 2024

Plastic pollution is an ever-escalating issue with detrimental effects on both the environment and human health. breaks down into smaller pieces, depending size they are called macroplastics, microplastics (MPs), nanoplastics (NPs). Some of these particles can easily enter food chain causing toxicity to many plants animals. The extensive use synthetic polymers such as polyethylene (PE), polyvinyl chloride (PVC), polystyrene (PS), terephthalate (PET) poses substantial environmental concerns due their degradation-resistant characteristics. One ways microorganisms address this by producing enzymes. This review examines recent advancements in enzymatic degradation commercial-grade pure polymers, including effectiveness enzymes laccases, proteases, cutinases, PETase, MHETase, governing mechanisms across various plastic categories. Bioinformatic tools multi-omics, molecular docking, enzyme mining particularly useful identifying unconventional biocatalysts plastic-degrading microbes a culture-independent manner. Furthermore, techniques enhance catalytic efficiency degrading (PDEs) using modern approaches protein engineering, mutations, chimeric fusion, etc. have also been reviewed. accentuates pivotal role microbial mitigating pollution, associated challenges, suitable prospects achieve closed-loop recycling future.

Language: Английский

Citations

6
Upcycling of recycled polyethylene for rotomolding applications via dicumyl peroxide crosslinking DOI Creative Commons
Hibal Ahmad, Denis Rodrigue

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Abstract Polyethylene (PE), including high‐density polyethylene (HDPE) and low‐density (LDPE), makes up a significant part of post‐consumer plastics in municipal solid waste, presenting challenges for traditional recycling methods due to wide range melt flow properties poor interfacial adhesion between the different resin, which often leads low quality products (downcycling). In this study, method is proposed modify molecular structure PE (rHDPE rLDPE) their blends by using straightforward organic peroxide crosslinking technique with 1 phr dicumyl (DCP). Different rHDPE/rLDPE blend weight ratios (0/100, 20/80, 40/60, 50/50, 60/40, 80/20, 100/0) were prepared combination co‐rotating twin‐screw extrusion pulverization. The final parts produced via rotomolding where both forming processes occurred concurrently. Subsequently, materials characterized terms chemical, thermal, mechanical properties. It was found that tensile strength (228%), modulus (345%), flexural (145%), (251%) increased 80% wt. rHDPE (x‐rHDPE). Conversely, gel content 17%, thermal resistance 37.2%, impact 93% rLDPE (x‐rLDPE). can be concluded balance occurs as addition DCP improved blends. This innovative approach represents simple upcycle mixed (PE) streams, especially applications. also offers promising avenues sustainable waste management material reuse.

Language: Английский

Citations

4
Collaborating for Impact: Navigating Partnerships and Overcoming Challenges across the Sustainable Development Goals DOI Creative Commons

Hiba Azim,

Amy-Louise Johnston,

Morag Nixon

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

We illustrate the importance of early career perspectives and diverse partnerships to develop solutions overcome key challenges achieve Sustainable Development Goals.

Language: Английский

Citations

0
Construction of Yarrowia lipolytica for degradation of low-density polyethylene DOI
Fei Liu, Ni Zhang, Yutong Shang

et al.

Process Safety and Environmental Protection, Journal Year: 2025, Volume and Issue: unknown, P. 106818 - 106818

Published: Jan. 1, 2025

Language: Английский

Citations

0
High-throughput screening strategies for plastic-depolymerizing enzymes DOI

Maxine Yew,

Yifan Yang, Qinhong Wang

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biocatalytic recycling of plastics: facts and fiction DOI Creative Commons
Wolfgang Zimmermann

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Enzymatic recycling of plastic waste is attracting considerable attention as a novel strategy to advance circular economy. While currently limited polyesters, achieving economically viable biocatalytic processes remains key challenge.

Language: Английский

Citations

0