Multi-omics insights into the response ofAspergillus parasiticusto long-chain alkanes in relation to polyethylene degradation DOI Creative Commons
Romanos Siaperas, George Taxeidis, Efstratios Nikolaivits

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Plastic pollution presents a global challenge, with polyethylene (PE) being among the most persistent plastics due to its durability and environmental resilience. In this study, we employed multi-omics approach study ability of Aspergillus parasiticus MM36, an isolate derived from Tenebrio molitor intestines, metabolize long-chain alkanes (lcAlk) secrete enzymes able modify PE. The fungus was grown hexadecane (C16) or mixture lcAlk (C24 C36) as carbon sources culture supernatants were tested daily for their Proteomic analysis identified induced oxidases potentially involved in PE functionalization. Key include multicopper oxidases, peroxidases, unspecific peroxygenase FAD-dependent monooxygenases. Surfactant proteins facilitating enzymatic cellular interaction hydrophobic lcAlk, such one hydrophobin, three surface-binding (HsbA) cerato platanin, present all secretomes. Transcriptomic comparing C16 cultures highlighted enrichment oxidoreductase activities carboxylic acid metabolism both incubation days, transmembrane transporters transferases predominating on day 2 biosynthetic processes 3. cultures, hydrolytic enzymes, including esterases, upregulated alongside Baeyer-Villiger monooxygenases, suggesting shift toward sub-terminal hydroxylation. Integrating transcriptomic secretomic data, propose mechanism assimilation by A. involving extracellular oxyfunctionalization, hydrocarbon uptake via surface-modifying channeling through membrane energy consumption processes. This provides insights into fungal mechanisms alkane highlights relevance plastic degradation. Importance challenge marine life human health, Hydroxylation is regarded initial step degradation, similar oxidation, making alkane-degrading microbes promising source degraders. used investigate MM36 that secretomes key biosurfactants enable interact transform substrates like further revealed biological metabolism. By integrating these insights, highlight biodegradation. work advances our understanding contributions addressing pollution.

Language: Английский

Polyethylene biodegradation: A multifaceted approach DOI Creative Commons

Anjali Purohit,

Bastien Cochereau,

Omprakash Sarkar

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108577 - 108577

Published: April 1, 2025

Language: Английский

Citations

0
Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes DOI Creative Commons
Anton A. Stepnov, Esteban Lopez-Tavera, Ross R. Klauer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 15, 2024

Abstract Biocatalytic degradation of non-hydrolyzable plastics is a rapidly growing field research, driven by the global accumulation waste. Enzymes capable cleaving carbon-carbon bonds in synthetic polymers are highly sought-after as they may provide tools for environmentally friendly plastic recycling. Despite some reports oxidative enzymes acting on plastics, including polyethylene or poly(vinyl chloride), notion that these materials susceptible to efficient enzymatic remains controversial, partly general lack studies independently reproducing previous observations. We attempted replicate two recent reporting deconstruction and chloride) can be achieved using an insect hexamerin from Galleria mellonella (so-called “Ceres”) bacterial catalase-peroxidase Klebsiella sp. , respectively. Reproducing previously described experiments with recombinant proteins, we did not observe any activity multiple reaction conditions substrate types. Digging deeper into discrepancies between data our observations, show how why original experimental results have been misinterpreted, leading erroneous claim had occurred. Our should lead caution when interpreting amount literature claiming plastics.

Language: Английский

Citations

3
Current paradigms and future challenges in harnessing gut bacterial symbionts of insects for biodegradation of plastic wastes DOI
Mudasir A. Dar, Rongrong Xie, Hossain M. Zabed

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: July 11, 2024

Abstract The ubiquitous incorporation of plastics into daily life, coupled with inefficient recycling practices, has resulted in the accumulation millions metric tons plastic waste, that poses a serious threat to Earth's sustainability. Plastic pollution, global problem, disrupts ecological balance and endangers various life forms. Efforts combat pollution are underway, promising avenue being biological degradation facilitated by certain insects their symbiotic gut microorganisms, particularly bacteria. This review consolidates existing knowledge on influence microbiota. Additionally, it delves potential mechanisms employed symbiosis bacteria, exploring bioconversion waste value‐added biodegradable polymers through mineralization. These insights hold significant promise for bio‐upcycling opening new horizons future biomanufacturing high‐value chemicals from plastic‐derived compounds. Finally, we weigh pros cons research endeavors related bioprospection plastic‐degrading bacteria underexplored insect species. We also underscore importance bioengineering depolymerases novel characteristics, aiming application remediation valorization plastics.

Language: Английский

Citations

3
Interfacial Reactions in Chemical Recycling and Upcycling of Plastics DOI Creative Commons
Albert Ong, Jerald Y. Q. Teo, Jason Y. C. Lim

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 46975 - 46987

Published: Aug. 30, 2024

Depolymerization of plastics is a leading strategy to combat the escalating global plastic waste crisis through chemical recycling, upcycling, and remediation micro-/nanoplastics. However, critical processes necessary for polymer chain scission, occurring at polymer–catalyst or polymer–fluid interfaces, remain largely overlooked. Herein, we spotlight importance understanding these interfacial as necessity optimizing kinetics reactivity in recycling controlling reaction outcomes, product distributions, well improving environmental sustainability processes. Several examples are highlighted heterogeneous such hydrogenation over solid catalysts, immiscible media, biocatalysis. Ultimately, judicious exploitation has practical implications developing practical, robust, cost-effective reduce enable viable post-use circular economy.

Language: Английский

Citations

3
Isolation of marine polyethylene (PE)-degrading bacteria and its potential degradation mechanisms DOI
Qian Meng,

Xianliang Yi,

Hao Zhou

et al.

Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 207, P. 116875 - 116875

Published: Sept. 4, 2024

Language: Английский

Citations

3
Microbial degradation of polyethylene polymer: current paradigms, challenges, and future innovations DOI
Babbiker Mohammed Taher Gorish,

Waha Ismail Yahia Abdelmula,

Sivasamy Sethupathy

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(12)

Published: Dec. 1, 2024

Language: Английский

Citations

2
Biodegradation of oxidized low density polyethylene by Pelosinus fermentans lipase DOI Creative Commons
D. Kim, Eui Seok Lim, Ga Hyun Lee

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 403, P. 130871 - 130871

Published: May 21, 2024

Polyethylene (PE) exhibits high resistance to degradation, contributing plastic pollution. PE discarded into the environment is photo-oxidized by sunlight and oxygen. In this study, a key enzyme capable of degrading oxidized reported for first time. Twenty different enzymes from various lipase families were evaluated hydrolytic activity using substrates mimicking PE. Among them, Pelosinus fermentans 1 (PFL1) specifically cleaved ester bonds within carbon-carbon backbone. Moreover, PFL1 (6 μM) degraded film, reducing weight average number molecular weights 44.6 11.3 %, respectively, five days. Finally, structural analysis docking simulations performed elucidate degradation mechanism PFL1. The PE-degrading here will provide groundwork advancing waste treatment technology engineering microbes repurpose valuable chemicals.

Language: Английский

Citations

1
Nature-Inspired Strategies for Sustainable Degradation of Synthetic Plastics DOI Creative Commons
Sreeahila Retnadhas, Daniel C. Ducat, Eric L. Hegg

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(9), P. 3323 - 3339

Published: Aug. 27, 2024

Synthetic plastics have become integral to our daily lives, yet their escalating production, limited biodegradability, and inadequate waste management contribute environmental contamination. Biological plastic degradation is one promising strategy address this pollution. The inherent chemical physical properties of synthetic plastics, however, pose challenges for microbial enzymes, hindering the effective development a sustainable biological recycling process. This Perspective explores alternative, nature-inspired strategies designed overcome some key limitations in currently available plastic-degrading enzymes. Nature's refined pathways natural polymers, such as cellulose, present compelling framework efficient technologies enzymatic degradation. By drawing insights from nature, we propose general employing substrate binding domains improve targeting multienzyme scaffolds efficiency limitations. As potential application, outline pathway upcycle polyethylene into alkenes. Employing can path toward solution impact plastics.

Language: Английский

Citations

1
Characterization of Potential Plastic-Degradation Enzymes from Marine Bacteria DOI Creative Commons
Jin Jin, Zongchao Jia

ACS Omega, Journal Year: 2024, Volume and Issue: 9(29), P. 32185 - 32192

Published: July 8, 2024

Polyethylene terephthalate (PET) and polyethylene (PE) are prominent polymer materials that comprise a significant portion of commercial plastic waste. Their durability slow degradation rate have resulted in accumulation on Earth. In recent study, macrotranscriptomic profiling reconstituted marine bacterial community identified 10 putative enzymes capable directly acting PE or PET (PEases PETases). Among these enzymes, three recombinant proteins were reported to possess activity. To select potential degrading enzyme candidates for protein engineering efforts, we expressed purified eight out the candidates, excluding two due poor expression and/or solubility. Notably, several candidate displayed esterase activity p-nitrophenyl butyrate exhibited unexpected thermostability despite their origin. Additionally, observed dose- time-dependent hydrolytic trimer substrate. Structural analysis mutagenesis confirmed presence catalytic triad residues, classifying it as an esterase. Furthermore, elucidated structural importance disulfide bonds. Through point mutation experiments, enhanced selected nanoparticles. Our findings challenge classification highlight significance complexity validating through metagenomic analysis.

Language: Английский

Citations

0
Polyethylene Biodegradation by an Artificial Bacterial Consortium: <i>Rhodococcus</i> as a Competitive Plastisphere Species DOI Open Access

Jyothi Priya Putcha,

Wataru Kitagawa

Microbes and Environments, Journal Year: 2024, Volume and Issue: 39(3), P. n/a - n/a

Published: Jan. 1, 2024

Polyethylene (PE), a widely used recalcitrant synthetic polymer, is major global pollutant. PE has very low biodegradability due to its rigid C-C backbone and high hydrophobicity. Although microorganisms have been suggested possess PE-degrading enzymes, our understanding of the biodegradation process overall applicability still lacking. In present study, we an artificial bacterial consortium for compensate enzyme availability metabolic capabilities individual strains. Consortium members were selected based on available literature preliminary screening including laccases, lipases, esterases, alkane hydroxylases. pellets incubated with 200 days. A next-generation sequencing ana­lysis community culture broth pellet identified Rhodococcus as dominant bacteria. Among strains in consortium, erythropolis was predominant. Scanning electron microscopy (SEM) revealed multilayered biofilms bacteria embedded surface. SEM micrographs after biofilm removal showed pitting surface deterioration. Multicellular structures biodeterioration observed incubation R. alone. The study demonstrated that may be biodegraded by artificially constructed which emerged important player. results showing robust colonization hydrophobic it naturally possesses extracellularly expresses several target enzymes suggest potential host further improved genetic engineering technology using well-studied host-vector system.

Language: Английский

Citations

0