International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
26(1), P. 5 - 5
Published: Dec. 24, 2024
Di-(2-ethylhexyl)
phthalate
(DEHP)
and
Cadmium
(Cd)
affect
female
reproduction.
To
date,
toxicological
research
has
focused
on
the
effects
of
individual
contaminants,
whereas
living
beings
are
exposed
to
mixtures.
This
study
analyzed
a
DEHP/Cd
mixture
nuclear
cytoplasmic
maturation
sheep
cumulus–oocyte
complexes
(COCs)
compared
with
single
compounds.
COCs
recovered
from
slaughterhouses-derived
ovaries
were
in
vitro
0.5
μM
DEHP,
0.1
Cd,
or
at
same
concentrations
during
24
h
(IVM).
After
IVM,
oocyte
chromatin
configuration
was
evaluated,
bioenergetic/oxidative
parameters
assessed
expanded
cumulus
cells
(CCs)
matured
oocytes
(chi-square
test
one-way
ANOVA;
p
<
0.05).
Under
examined
conditions,
never
impaired.
However,
COC
bioenergetics
affected
stronger
for
than
Indeed,
percentages
healthy
mitochondrial
distribution
patterns
reduced
(p
0.001
0.05
compounds,
respectively).
Oocyte
membrane
potential,
intracellular
ROS
levels,
mitochondria/ROS
co-localization
reduced,
significance
level,
all
contaminated
conditions.
CCs
displayed
increased
levels
only
upon
exposure
0.001).
In
conclusion,
quality
greater
extent
separate
