Identification and Characterization of a Novel Nitroreductase Transforming the Herbicide Mesotrione in Metabacillus sp. JX24 DOI
Bin Liu,

Yingying Wan,

Miaohuan Xiong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

Mesotrione, a commonly used 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, threatens agro-ecosystem sustainability and nontarget organisms. Microbes play significant role in the biodegradation of mesotrione. However, an understanding molecular mechanisms involved mesotrione degradation is still quite limited. Here, bacterial strain Metabacillus sp. JX24 capable degrading was isolated. The could effectively degrade about 98% 100 μM within 12 h. It revealed that degraded through successive reduction its nitro group to generate 2-amino-4-methylsulfonylbenzoic acid (AMBA). A gene, mnrA, encoding novel nitroreductase MnrA responsible for transformation mesotrione, cloned from JX24. shared low sequence identities (<20%) with reported nitroreductases. purified catalyzed NADH or NADPH as cofactors vitro. Km kcat/Km values were 22.0 0.57 μM-1 min-1, respectively. detoxification assay indicated AMBA, product MnrA-mediated reduction, did not inhibit HPPD activity, suggesting confers detoxifying action on Our work provides enzymatic resource elimination residues environment.

Language: Английский

Unveiling the hydrolase Oph2876 mediated chlorpyrifos degradation mechanism in Pseudomonas nitroreducens and its potential for environmental bioremediation DOI
Haoran Song, Wenjuan Chen, Shao-Fang Chen

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136570 - 136570

Published: Nov. 19, 2024

Language: Английский

Citations

4
Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential DOI Creative Commons
Hui Liu, Wenjuan Chen, Zeling Xu

et al.

Environment International, Journal Year: 2024, Volume and Issue: 195, P. 109221 - 109221

Published: Dec. 17, 2024

Language: Английский

Citations

4
Effects of herbicide mixtures on the diversity and composition of microbial community and nitrogen cycling function on agricultural soil: a field experiment in Northeast China DOI
Qing‐Yun Ma, Yiqing Zhou,

Rebecca E. Parales

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: 372, P. 125965 - 125965

Published: March 3, 2025

Language: Английский

Citations

0
Elucidation of Molecular Mechanisms Underlying Degradation of Nicosulfuron and Its Derivative by Klebsiella jilinsis 2N3 Using Multiomic Analysis DOI
Qianhang Zhai, Yue Liu, Lining Zheng

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137838 - 137838

Published: March 5, 2025

Language: Английский

Citations

0
The alteration in adsorption mechanism and associated bioaccessibility of mesotrione on virgin and aged biodegradable mulch film-derived microplastics DOI

Hua Yang,

Jie Yang,

Liwen Sun

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163821 - 163821

Published: May 1, 2025

Language: Английский

Citations

0
Identification and Characterization of a Novel Nitroreductase Transforming the Herbicide Mesotrione in Metabacillus sp. JX24 DOI
Bin Liu,

Yingying Wan,

Miaohuan Xiong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

Mesotrione, a commonly used 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, threatens agro-ecosystem sustainability and nontarget organisms. Microbes play significant role in the biodegradation of mesotrione. However, an understanding molecular mechanisms involved mesotrione degradation is still quite limited. Here, bacterial strain Metabacillus sp. JX24 capable degrading was isolated. The could effectively degrade about 98% 100 μM within 12 h. It revealed that degraded through successive reduction its nitro group to generate 2-amino-4-methylsulfonylbenzoic acid (AMBA). A gene, mnrA, encoding novel nitroreductase MnrA responsible for transformation mesotrione, cloned from JX24. shared low sequence identities (<20%) with reported nitroreductases. purified catalyzed NADH or NADPH as cofactors vitro. Km kcat/Km values were 22.0 0.57 μM-1 min-1, respectively. detoxification assay indicated AMBA, product MnrA-mediated reduction, did not inhibit HPPD activity, suggesting confers detoxifying action on Our work provides enzymatic resource elimination residues environment.

Language: Английский

Citations

0