Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent reproducibility, interrupt the timely prevention of disease spread development medical devices for home outdoor uses. A newly fabricated gold nanopillar array-based film is presented superior photothermal energy conversion. Magnifying metal surface-to-volume ratio increases conversion efficiency, resulting in a swift reduction gene amplification reaction time. Plasmonic energy-based ultrafast facile confirmation methodology offer rapid discrimination platform high-throughput multiplexed diagnosis. The performance arrayed demonstrated by measuring amount pathogen (Vibrio cholerae) with sensitivity 101 cfu mL-1 5.5 min. engineered can be utilized to diagnose universal pathogens achieve an increasingly successful complete cure.

Language: Английский

Distribution characteristics of aerosol microorganisms in bronchoscopy room and the risk assessment of nosocomial infection

Frontiers in Public Health, Journal Year: 2025, Volume and Issue: 13

Published: April 28, 2025

A large number of aerosols containing pathogenic microorganisms can be produced during bronchoscopy. The aim the study is to evaluate risk nosocomial infection by after distribution characteristics aerosol were detected before and bronchoscopy, then compared with patients' respiratory pathogens. total 152 patients underwent bronchoscopy in room from May 06, 2024 June 30, 2024. Airborne collected cultured, counted identified, analyze species, numbers changes microorganisms. At same time, data pathogens all correlation between air pathogens, infection. (1) concentration was 89.60 ± 63.52 CFU/m3, significantly higher than 43.80 26.70 CFU/m3 (p < 0.001). increase microorganism accordance infectious diseases on day After for diseases, that non-infectious 0.05). (2) bacteria accounted 75.34, 70.35% microorganisms, fungi 22.17, 26.80% mainly included Micrococcus luteus, Staphylococcus epidermidis, hominis, Haemophilus influenzae, Neisseria faunalis, capitis, etc. Aspergillus flavus, niger, Saccharomyces albicans, Penicillium spp., (3) consistent causing infections increased composed common but it did not = 0.735). Bronchoscopy are related patients, which pulmonary This, however, does

Language: Английский

Ultralow-cost personal PCRstrip with volumetric heating based on broadband absorption plasmonic fabric for POCT of pathogens

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

The diagnosis of respiratory viral infection via reverse transcription-polymerase chain reaction (RT-PCR) is typically conducted in centralized laboratories using bulky equipment costing 1–2 h. To prevent the spread infectious diseases, there an urgent need for ultrafast and accessible molecular diagnostic tools point-of-care testing (POCT). Here, we developed ultrafast, POC PCRstrip capable detecting influenza A RNA with high sensitivity (1 copy/µL) just 15 min. Our system integrated transcription, rapid thermocycling visual detection at ultralow cost. Broadband absorption plasmonic fabric (BAPF) excellent photothermal effect was fabricated volumetric heating excited by a cost-effective white LED. After PCR, amplification products can be visually detected lateral flow paper strip. Clinical validation 80 nasopharyngeal swab samples collected from patients suspected demonstrated clinical 98.4% specificity 94.7%. This fast, ultralow-cost reliable strategy conducive to POCT, offering effective tool non-trained personnel detect control diseases individually.

Language: Английский