Generalization of Classification of AlkB Family Alkane Monooxygenases from Rhodococcus (sensu lato) Group Based on Phylogenetic Analysis and Genomic Context Comparison

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1713 - 1713

Published: Feb. 17, 2025

Alkane-oxidizing bacteria play a crucial role in the global carbon cycle. Rhodococcus species are well-known hydrocarbon degraders, distinguished by harboring of multiple homologs AlkB family alkane monooxygenases. Although different types rhodococcal AlkBs have been described, overall picture their diversity remains unclear, leaving gaps current classification. We conducted phylogenetic analysis all identified (sensu lato) and examined genomic context corresponding genes. The sequence clustering was well aligned with neighborhoods, allowing both features to be used as criteria for proposing that form distinct groups characteristic contexts. Our approach allowed us revise classification previously described AlkBs, identifying eight on basis, propose three new ones. Alkane monooxygenases whose genes co-localized rubredoxin can considered generalized AlkBR type, most common among Rhodococcus. In AlkB0 which is paralog AlkBR, violations conservativity known monooxygenase signature motifs were found. findings provide more consistent framework prevents over-reporting "novel" contributes deeper understanding diversity.

Language: Английский

Endophytic Bacterial Communities Facilitate the Dissipation of Phthalates in Soil and Their Biodegradation in Oryza Sativa L.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

The role of endophytic bacterial communities in aiding the degradation organic pollutants like phthalates (PAEs) soil and planta, as well their effects on pollutant accumulation plants, remains unclear. Herein, microcosm experiments were conducted with rice cultivated agricultural polluted di(2-ethylhexyl) phthalate (DEHP) further verified PAE-degrading consortia. Soil indigenous microbes, especially bacteria, significantly contributed to DEHP dissipation diminished rice. Endophytic participated validated by efficient vitro culturable consortia abundant genes. inoculation demonstrated immigration between roots (especially low-PAE-accumulating cultivar), which enhanced planta subsequently reduced PAE accumulation. This study underscores facilitative lowering crops.

Language: Английский