Long-Term Real-Time Tracking of Morphology and Migration of Neuronal Cells under Oxidative Stress DOI Creative Commons
Mingkang Li, Ru‐Jia Yu, Kele Chen

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 3(3), P. 191 - 198

Published: Nov. 19, 2024

Neuronal cells exhibit diverse morphologies that are crucial for their function within the neuronal network. Long-term quantitative analysis of both cell morphology and migration is essential in neuroscience research but remains challenging. Sodium arsenite, a known inducer oxidative stress neurons, affects migration. To rapidly assess HT22 cells, we developed method tracking key morphological features trajectories individual cells. Three time-dependent parameters─velocity, circularity increment, turn angle─are identified as rapid, direct indicators early stages This then applied to investigate effects arsenite exposure on Our approach provides valuable tool label-free, long-term real-time offering potential insights into cellular responses under conditions.

Language: Английский

Association of miR-21 gene polymorphisms with cognitive function in patients with systemic lupus erythematosus DOI
Tiantian Wei,

Jing Shen,

Lijun He

et al.

Personalized Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 7

Published: March 14, 2025

The genetic variant rs13137 of miR-21 is associated with susceptibility in many diseases. However, the association cognitive dysfunction (CD) Chinese patients systemic lupus erythematosus (SLE) remains unclear. Two hundred and thirty SLE (Non-CD) 230 SLE-related CD were recruited. MiR-21 level was calculated by qRT-PCR. ROC curve established to evaluate diagnosibility. independent risk factors identified multivariate logistic regression analysis. group obviously increased. Compared AA carriers, carriers AT/TT significantly lower than those Non-CD group. AUC 0.9023 sensitivity 78.70% specificity 90.87%. Comparison genotype allele frequencies indicated that carrying had low CD. Multivariate analysis showed polymorphism, education years, MoCA score correlated risk. polymorphism population. patients.

Language: Английский

Citations

0
Long-Term Real-Time Tracking of Morphology and Migration of Neuronal Cells under Oxidative Stress DOI Creative Commons
Mingkang Li, Ru‐Jia Yu, Kele Chen

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 3(3), P. 191 - 198

Published: Nov. 19, 2024

Neuronal cells exhibit diverse morphologies that are crucial for their function within the neuronal network. Long-term quantitative analysis of both cell morphology and migration is essential in neuroscience research but remains challenging. Sodium arsenite, a known inducer oxidative stress neurons, affects migration. To rapidly assess HT22 cells, we developed method tracking key morphological features trajectories individual cells. Three time-dependent parameters─velocity, circularity increment, turn angle─are identified as rapid, direct indicators early stages This then applied to investigate effects arsenite exposure on Our approach provides valuable tool label-free, long-term real-time offering potential insights into cellular responses under conditions.

Language: Английский

Citations

0