Cascaded Gravity-Driven Microfluidic Chip with Tilt-Actuated Siphon Valves for Rapid Detection of Salmonella Typhimurium DOI

Fengzhen Yang,

Tianchang Wang,

Siyuan Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

In recent years, urgent food safety issues have heightened the demand for rapid detection technologies foodborne pathogens, especially biosensors featuring simplicity, rapidity, and high sensitivity. Yet despite a booming surge in related published studies, commercializing these remains constant challenge persistent objective researchers. this study, gravity-driven microfluidic chip with tilt-actuated siphon valves was developed, integrating silica magnetic beads based nucleic acid separation recombinase-aided amplification (RAA) of Salmonella Typhimurium by simple operations along portable biosensing device. By inclination, balance between gravity reagents capillary force around is altered; thus, tilting could provide driving fluid flow without need external pumps. Siphon were designed geometrically surface modifications to generate comfortable angles, holder built-in angle guide ensure consistent operational performance. A smartphone app developed monitor fluorescence signals quantitative S. Typhimurium. Experimental results showed that device detect spiked chicken samples at concentrations as low 1.1 × 101 CFU/mL within 60 min, recovery rates ranging from 91.54% 117.27%. The also demonstrated compatibility diverse liquid properties, offering scalability adaptability various scenarios potential pathogens clinical diagnostics using probes reagents.

Language: Английский

The regulation of tobacco growth under preceding crop planting: insights from soil quality, microbial communities, and metabolic profiling DOI Creative Commons
Peiyan Zhao,

Houfa Zhou,

Xiaolin Liao

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Specific microorganisms and metabolites in soil play key roles regulating organismal behavior. Currently, the effects of different preceding crops on rhizosphere quality flue-cured tobacco remain unclear. Four treatments were compared study: fallow + (CK), maize (T1), rapeseed (T2), wheat (T3). Results showed that significantly enhanced nutrient levels improved growth by altering microbial community structure. Previous cultivation promoted growth, diversity bacterial communities, notably decreased abundance urea-degrading bacteria. In contrast, crop reduced plant pathogenic fungi positive interactions. Metabolomics analysis altered lipids, organic acids, flavonoids, alkaloids, terpenoids, enhancing secondary metabolite synthesis pathways soil. Preceding regulated which potentially participated carbon nitrogen cycling, balancing nutrients, improving yield. Overall, three composition function structures soil, thereby increased concentration. Both boosted biomass. These findings offer new insights into potential interactions between communities strategies comprehensively growth.

Language: Английский

Citations

0
Cascaded Gravity-Driven Microfluidic Chip with Tilt-Actuated Siphon Valves for Rapid Detection of Salmonella Typhimurium DOI

Fengzhen Yang,

Tianchang Wang,

Siyuan Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

In recent years, urgent food safety issues have heightened the demand for rapid detection technologies foodborne pathogens, especially biosensors featuring simplicity, rapidity, and high sensitivity. Yet despite a booming surge in related published studies, commercializing these remains constant challenge persistent objective researchers. this study, gravity-driven microfluidic chip with tilt-actuated siphon valves was developed, integrating silica magnetic beads based nucleic acid separation recombinase-aided amplification (RAA) of Salmonella Typhimurium by simple operations along portable biosensing device. By inclination, balance between gravity reagents capillary force around is altered; thus, tilting could provide driving fluid flow without need external pumps. Siphon were designed geometrically surface modifications to generate comfortable angles, holder built-in angle guide ensure consistent operational performance. A smartphone app developed monitor fluorescence signals quantitative S. Typhimurium. Experimental results showed that device detect spiked chicken samples at concentrations as low 1.1 × 101 CFU/mL within 60 min, recovery rates ranging from 91.54% 117.27%. The also demonstrated compatibility diverse liquid properties, offering scalability adaptability various scenarios potential pathogens clinical diagnostics using probes reagents.

Language: Английский

Citations

0