Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 972 - 972

Published: April 24, 2025

Groundwater harbors phylogenetically diverse Candidate Phyla Radiation (CPR) bacteria, representing an ideal ecosystem for studying this microbial dark matter. However, no CPR strains have been successfully isolated from groundwater, severely limiting further research. This study employed a multi-metagenome approach, integrating time-resolved sampling, antibiotic/nutrient interventions, and correlation networks to unravel ecological roles in groundwater provide insights into their subsequent cultivation. Through 36 metagenomes system containing at least 68 phyla, we revealed the time-sensitive collapse of communities: total abundance plummeted 7.9% 0.15% within 48 h post-sampling, driven by competition with rapidly dividing non-CPR such as members Pseudomonadota. Ampicillin (100 mg/L) stabilized communities suppressing competitors, whereas low-nutrient conditions paradoxically reversed effect. Long-term enrichment (14 months) recovered 63 phyla (0.35% abundance), revealing survival resilience despite nutrient deprivation. Correlation prioritized Actinomyces, novel Acidimicrobiaceae genus, Aestuariivirga, Baekduia Caedimonadaceae potential partners, providing actionable targets co-culture trials. Here, propose recommendations spanning activation status, identification symbiotic optimization culture conditions, which bypass traditional blind cultivation are critical future efforts cultivate bacterial groundwater. Cultivating bacteria will contribute clarifying diversity, roles, evolutionary mechanisms, metabolic pathways, genetic potential.

Language: Английский