Surfactin inhibits enterococcal biofilm formation via interference with pilus and exopolysaccharide biosynthesis

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 24, 2025

Enterococcus faecalis is a significant pathogen in healthcare settings and frequently resistant to multiple antibiotics. This resistance compounded by its ability form biofilms, dense bacterial communities that are challenging eliminate via standard antibiotic therapies. As such, targeting biofilm formation considered viable strategy for addressing these infections. study assessed the effectiveness of surfactin, cyclic lipopeptide biosurfactant synthesized Bacillus subtilis natto NTU-18, preventing E. faecalis. Analytical characterization surfactin was performed liquid chromatography‒mass spectrometry (LC‒MS). Additionally, transcriptomic sequencing quantitative PCR (qPCR) were used investigate alterations gene expression following treatment with surfactin. The data revealed notable suppression crucial virulence-related genes responsible pilus construction exopolysaccharide synthesis, both which vital adhesion structure. Functional tests confirmed substantially reduced attachment Caco-2 cell monolayers curtailed production. Moreover, confocal laser scanning microscopy thinning biofilms. These observations support potential utility as therapeutic agent manage biofilm-associated infections caused

Language: Английский

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Systematic investigation of catechin/cyclodextrin encapsulated polymeric nanospheres for the treatment and nursing care of pediatric pneumonia-causing human pathogens

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107524 - 107524

Published: April 1, 2025

Language: Английский

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Unveiling the potential antibacterial action of acetylcysteine for managing Staphylococcus aureus wound infections: in vitro and in vivo study

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: April 1, 2025

Language: Английский

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Disarming Staphylococcus aureus: Review of Strategies Combating This Resilient Pathogen by Targeting Its Virulence

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 386 - 386

Published: April 15, 2025

Staphylococcus aureus is a formidable pathogen notorious for its antibiotic resistance and diverse virulence mechanisms, including toxin production, biofilm formation, immune evasion. This article explores innovative anti-virulence strategies to disarm S. by targeting critical factors without exerting bactericidal pressure. Key approaches include inhibiting adhesion neutralizing toxins, disrupting quorum sensing (e.g., Agr system inhibitors), blocking iron acquisition pathways. Additionally, interventions two-component regulatory systems are highlighted. While promising, challenges such as strain variability, resilience, pharmacokinetic limitations, evolution underscore the need combination therapies advanced formulations. Integrating with traditional antibiotics host-directed offers sustainable solution combat multidrug-resistant aureus, particularly methicillin-resistant strains (MRSA), mitigate global public health crisis.

Language: Английский

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Enhanced bioavailability and improved antimicrobial, antibiofilm, and antivirulence activities of fish gelatin-based nanoformulations prepared by coating of maltol-gold nanoparticles

Chemosphere, Journal Year: 2025, Volume and Issue: 379, P. 144439 - 144439

Published: April 26, 2025

Language: Английский

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Antibiofilm Activities of Multiple Halogenated Pyrimidines Against Staphylococcus aureus

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12830 - 12830

Published: Nov. 28, 2024

, prevalent in hospital and community settings, forms biofilms that are highly resistant to antibiotics immune responses, complicating treatment contributing chronic infections. These challenges underscore the need for novel treatments target biofilm formation effectively reduce bacterial virulence. This study investigates antibiofilm antimicrobial efficacy of halogenated pyrimidine derivatives against

Language: Английский

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Marine polysaccharides for antibiofilm application: A focus on biomedical fields

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137786 - 137786

Published: Nov. 1, 2024

Language: Английский

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