Transforming Poly(butylene adipate-co-terephthalate) into Protocatechuic Acid via Enzymatic Degradation and Whole-Cell Catalysis DOI
Yunhan Li,

Can Su,

Fanghui Hu

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Poly(butylene adipate-co-terephthalate) (PBAT) is a biodegradable plastic with increasing applications that degrades rapidly in composting, but its sole degradation limits residual value utilization. Therefore, new strategies for converting PBAT to valuable products are needed. In this study, enzymatic was applied hydrolyze PBAT, and an engineered Escherichia coli strain constructed utilized as whole-cell catalyst synthesize protocatechuic acid (PCA), platform compound, from products. By fine-tuning the overexpression of four enzymes biosynthetic pathway optimizing catalytic conditions, titer PCA increased 5.07-fold 5.28 ± 0.05 g/L, achieving 15.45 1.04 wt % conversion rate pure particles. Furthermore, strategy applicable commercial PBAT/poly(lactic acid) (PLA), maximum 25.05 1.21 %. Thus, novel utilize waste established, which provides reference upgrading other macrowaste resources.

Language: Английский

Construction of Yarrowia lipolytica for degradation of low-density polyethylene DOI
Fei Liu, Ni Zhang, Yutong Shang

et al.

Process Safety and Environmental Protection, Journal Year: 2025, Volume and Issue: unknown, P. 106818 - 106818

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transforming Poly(butylene adipate-co-terephthalate) into Protocatechuic Acid via Enzymatic Degradation and Whole-Cell Catalysis DOI
Yunhan Li,

Can Su,

Fanghui Hu

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Poly(butylene adipate-co-terephthalate) (PBAT) is a biodegradable plastic with increasing applications that degrades rapidly in composting, but its sole degradation limits residual value utilization. Therefore, new strategies for converting PBAT to valuable products are needed. In this study, enzymatic was applied hydrolyze PBAT, and an engineered Escherichia coli strain constructed utilized as whole-cell catalyst synthesize protocatechuic acid (PCA), platform compound, from products. By fine-tuning the overexpression of four enzymes biosynthetic pathway optimizing catalytic conditions, titer PCA increased 5.07-fold 5.28 ± 0.05 g/L, achieving 15.45 1.04 wt % conversion rate pure particles. Furthermore, strategy applicable commercial PBAT/poly(lactic acid) (PLA), maximum 25.05 1.21 %. Thus, novel utilize waste established, which provides reference upgrading other macrowaste resources.

Language: Английский

Citations

0