2-Ethylhexyl Diphenyl Phosphate Affects Steroidogenesis and Lipidome Profile in Human Adrenal (H295R) Cells DOI Creative Commons
Chander K. Negi, Darshak Gadara, Lola Bajard

et al.

Chemical Research in Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

The ever-increasing use of chemicals and the rising incidence adverse reproductive effects in modern environment have become an emerging concern. Several studies shown that environmental contaminants, such as organophosphate flame retardants (OPFRs), negatively impact health. To evaluate potential endocrine-related widely used priority-listed compound 2-Ethylhexyl diphenyl phosphate (EHDPP), we characterized its on adrenal steroidogenesis human adrenocortical (H295R) cells. cells were exposed to EHDPP (1 5 μM) for 48 h, production hormones, including progesterone, androstenedione, testosterone, estradiol, cortisol, aldosterone, was measured. In addition, LC-MS/MS-based lipidomics analysis done quantify intracellular lipid profiles, transcriptional assays performed examine expression genes related corticosteroidogenesis, metabolism, mitochondrial dynamics. Our findings indicate disrupts hormone regulation vitro, evidenced by increased aldosterone secretion. key corticosteroidogenic (CYP11B2, CYP21A1, 3β-HSD2, 17β-HSD1) upregulated significantly upon exposure. Intracellular revealed EHDPP-mediated disruption, reduced total cholesterol ester, sphingolipids, phospholipids, triglyceride species, saturated-monounsaturated lipids subspecies. These alterations accompanied decreased ACAT2 SCD1 gene expression. Moreover, a shift dynamics indicated MF1 FIS1 data suggest homeostasis, emphasizing endocrine-disrupting effects.

Language: Английский

2-Ethylhexyl Diphenyl Phosphate Affects Steroidogenesis and Lipidome Profile in Human Adrenal (H295R) Cells DOI Creative Commons
Chander K. Negi, Darshak Gadara, Lola Bajard

et al.

Chemical Research in Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

The ever-increasing use of chemicals and the rising incidence adverse reproductive effects in modern environment have become an emerging concern. Several studies shown that environmental contaminants, such as organophosphate flame retardants (OPFRs), negatively impact health. To evaluate potential endocrine-related widely used priority-listed compound 2-Ethylhexyl diphenyl phosphate (EHDPP), we characterized its on adrenal steroidogenesis human adrenocortical (H295R) cells. cells were exposed to EHDPP (1 5 μM) for 48 h, production hormones, including progesterone, androstenedione, testosterone, estradiol, cortisol, aldosterone, was measured. In addition, LC-MS/MS-based lipidomics analysis done quantify intracellular lipid profiles, transcriptional assays performed examine expression genes related corticosteroidogenesis, metabolism, mitochondrial dynamics. Our findings indicate disrupts hormone regulation vitro, evidenced by increased aldosterone secretion. key corticosteroidogenic (CYP11B2, CYP21A1, 3β-HSD2, 17β-HSD1) upregulated significantly upon exposure. Intracellular revealed EHDPP-mediated disruption, reduced total cholesterol ester, sphingolipids, phospholipids, triglyceride species, saturated-monounsaturated lipids subspecies. These alterations accompanied decreased ACAT2 SCD1 gene expression. Moreover, a shift dynamics indicated MF1 FIS1 data suggest homeostasis, emphasizing endocrine-disrupting effects.

Language: Английский

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