Tiliroside from Lagopsis supina Ameliorates Myocardial Ischemia Injury in Zebrafish by Activating the kdr-Mediated PI3K-Akt and MAPK Signaling Pathways

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2313 - 2313

Published: March 5, 2025

Lagopsis supina (Steph. ex. Willd.) Ikonn.-Gal., an ancient Chinese herbal medicine, is traditionally used to treat blood stasis diseases such as myocardial ischemia (MI). However, its pharmacodynamics substances of the anti-MI effect and their potential mechanisms remain unclear. This study aims elucidate effects L. against MI reveal underlying in zebrafish. LSD fraction was screened out for active from by isoprenaline hydrochloride (ISO)-induced It could increase stroke volume, ejection fraction, ventricular short-axis systolic rate zebrafish model. A total 30 compounds (Nos. 1-30) were isolated identified various chromatographic techniques nuclear magnetic resonance spectroscopy. Among them, six compounds, including three lignin 15, 16, 18) flavonoid glycosides 14, 25, 26), showed noticeable activities, tiliroside (No. 25) more active. Molecular docking indicated that has a strong binding ability with proteins KDR, PI3K, Akt, Erk, p38, Bcl-2, Bax, Caspase3. In end, results RT-qPCR manifested markedly upregulated expression levels genes kdr, pik3cb, akt2, mapk1, mapk11, mapk14, bcl-2b prominently downregulated bax caspase3. According above results, activated kdr-mediated PI3K-Akt MAPK signaling pathways exert activity. These discoveries give scientific basis applying treatment suggest new avenues developing candidate therapy.

Language: Английский

Network pharmacology-based study on the mechanism of Yiwei Decoction in chronic atrophic gastritis and experimental assessment

Journal of Holistic Integrative Pharmacy, Journal Year: 2024, Volume and Issue: 5(4), P. 277 - 290

Published: Dec. 1, 2024

Language: Английский