Characterization of the wheat-tetraploid Thinopyrum elongatum 7E(7D) substitution line with Fusarium head blight resistance DOI Creative Commons
Dandan Wu, Fei Wang, Linfeng Chen

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 26, 2024

Fusarium head blight (FHB), a devastating disease of wheat production, is predominantly elicited by graminearum (Fg). The tetraploid Thinopyrum elongatum tertiary gene resource common that possesses high affinity and displays resistance traits against multiple biotic abiotic stress. We aim to employ utilize the novel FHB resources from wild germplasm for breeding. Durum wheat-tetraploid Th. amphiploid 8801 was hybridized with cultivars SM482 SM51, F5 generation generated. conducted cytogenetically in situ hybridization (ISH) technologies select confirm genetically stable 7E(7D) substitution line K17-1069-5, which showed expansion both field greenhouse infection experiments displayed no significant disadvantage agronomic compared their parents field. F2 segregation populations (K17-1069-5 × SM830) 7E chromosome conferred dominant dosage effect. developed 19 SSR molecular markers specific 7E, could be genetic mapping large breeding marker-assisted selection (MAS) during procedures future. isolated Fhb7 allele (Chr7E) using homology-based cloning, designated as TTE7E-Fhb7. In summary, our study K17-1069-5 contains resistance.

Language: Английский

Priority actions for Fusarium head blight resistance in durum wheat: Insights from the wheat initiative DOI Creative Commons
Ambra Viviani, Jemanesh K. Haile, W. G. Dilantha Fernando

et al.

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 6, 2025

Fusarium head blight (FHB), mainly caused by graminearum and culmorum, is a major wheat disease. Significant efforts have been made to improve resistance FHB in bread (Triticum aestivum), but more work needed for durum turgidum spp. durum). Bread has ample genetic variation breeding, which can be readily exploited, while characterized higher disease susceptibility fewer valuable sources. The Wheat Initiative - Expert Working Group on Durum Genomics Breeding promoted scientific discussion define the key actions that should prioritized achieving comparable found wheat. Here, detailed state of art novel tools are presented, together with perspective next steps forward. A meta-analysis grouping all quantitative trait loci (QTL) associated both conducted identify hotspot regions do not overlap Rht alleles, known negatively correlate resistance. list QTL related deoxynivalenol contamination lines carrying different sources provided as strategic resource. QTL, closely linked markers useful selected design an effective breeding program. Finally, we highlight priority implemented achieve satisfactory

Language: Английский

Citations

1
Wheat-Psathyrostachys huashanica 4Ns Additional Line Confers Resistance to Fusarium Head Blight DOI Creative Commons
Yinghui Li,

Hang Peng,

Hao Zhang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1104 - 1104

Published: April 2, 2025

Fusarium head blight (FHB) is one of the major wheat diseases caused by species (mainly graminearum and asiaticum), resulting in significant global yield losses risks to food security. Breeding varieties with resistance genes most environmentally friendly economical strategy for controlling FHB. Psathyrostachys huashanica Keng ex P. C. Kuo (2n = 2x 14, NsNs), which showed abiotic tolerance biotic resistance, has research value potential as an important genetic resource improvement. In previous studies, we crossed common developed lines containing different NS chromosomes. this study, identified a 4NS additional line, DA26, from progenies wheat-P. huashanica-derived using genomic situ hybridization (GISH) fluorescence (FISH) analyses. Line DA26 high greenhouse field conditions. However, parental Chinese Spring (CS) CSph2b mutant susceptibility A evaluation agronomic traits that plant height was significantly lower than CS, while there were no differences other traits. addition, also eight 4Ns-specific primers identify 4Ns chromosome, can facilitate breeding FHB gene mapping future.

Language: Английский

Citations

0
Exploration of Genomic Regions Associated with Fusarium Head Blight Resistance in Wheat and Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers DOI Open Access

Pengbo Song,

Yueyue Li, Xin Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3339 - 3339

Published: April 3, 2025

Fusarium head blight (FHB), caused by graminearum, is a globally significant disease that severely impacts the yield and quality of wheat. Breeding resistant wheat varieties using resistance genes most cost-effective strategy for managing FHB, but few markers are available marker-assisted selection (MAS) resistance. In this study, we evaluated recombinant inbred line (RIL) population to FHB through single-floret inoculation in four field environments over two years. Combined with quantitative trait loci (QTL) detection high-density genetic mapping based on 50 K SNP arrays, identified total 21 QTLs influencing It worth noting QFhba-5D.2-1 was detected as well multi-environment trial (MET) analysis, explaining phenotypic variation ranging from 1.98% 18.55%. We also pinpointed thirteen within QTL intervals chromosomes 4A, 5D, 6B, 7A associated defense mechanisms. Furthermore, developed Kompetitive Allele-Specific PCR (KASP) QFhba-7A regions validate their specificity RIL population. Subsequently, validated polymorphism these 305 germplasms analyzed effect thousand kernel weight (TKW) spike length (SL). These will accelerate development FHB-resistant MAS, significantly reducing losses strengthening food security.

Language: Английский

Citations

0
Chromosome telosome 1StL from Elymus repens carries a novel locus Fhb.Er-1StL conferring resistance to Fusarium head blight in wheat DOI
Fei Wang, H. Wei, Qiong Wang

et al.

Published: April 14, 2025

Abstract Fusarium head blight (FHB) is a globally prevalent wheat disease caused by multiple species within Fusariumgenus. P1142-1-2 wheat-E. repens partial amphidiploid with high resistance to FHB. However, its detailed genome composition and the genetic information of chromatin derived from E. remains unclear, which limits transfer utilization potential gene into wheat. Based on GISH FISH karyotypes analysis, this study confirmed that possesses complete common seven homologous pairs chromosomes containing alien chromatin. The GenoBaits®Wheat-St 45K liquid microarray analysis revealed contains different St 1St 7St repens, respectively. Two FHB-resistant derivative lines were identified self-progenies P1142-1-2×Chuannong16 (a cultivar susceptible FHB) characterized as T1StL·1StL chromosome translocation line t1StL telosomic addition line, A total 17 molecular markers developed specific 1StL used construct physical map 1StL. Linkage demonstrated confers FHB distinct dosage effect. Diagnostic excludes presence known FHB R genes 1StL, it proposed harbors novel locus, designated Fhb.Er-1StL, enriches pool.

Language: Английский

Citations

0
Transcriptomic analysis of two Chinese wheat landraces with contrasting Fusarium head blight resistance reveals miRNA-mediated defense mechanisms DOI Creative Commons
Lijuan Wu, Junqiang Wang,

Shian Shen

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Introduction Fusarium head blight (FHB), caused primarily by graminearum ( Fg ), poses a significant threat to wheat production. It is necessary deeply understand the molecular mechanisms underlying FHB resistance in breeding. Methods In this study, transcriptomic responses of two Chinese landraces—Wuyangmai (WY, resistant) and Spring (CS, susceptible)—to F. infection were examined using RNA sequencing (RNA-seq). Differential expression mRNAs, long non-coding RNAs (lncRNAs), circular (circRNAs), microRNAs (miRNAs) was analyzed at 3 5 days post- inoculation (dpi). Results The results showed that WY exhibited targeted miRNA response, modulating defense-related pathways such as glutathione metabolism phenylpropanoid biosynthesis, which are crucial for oxidative stress regulation pathogen defense response. contrast, CS displayed broader transcriptional largely linked general metabolic processes rather than immune activation. Notably, up-regulation genes involved confirmed its enhanced FHB. integrated analysis miRNA-mRNA interactions highlighted miRNAs central regulators WY, particularly later stages infection. These responses, while lncRNAs circRNAs played more limited role responses. GO KEGG pathway enrichment analyses further revealed enriched plant-pathogen interaction secondary metabolite biosynthesis pathways, resistance. prioritized homeostasis, suggesting less effective strategy. Discussion Overall, study underscores critical miRNA-mediated WY. insights into regulatory provide basis breeding FHB-resistant varieties highlight promising targets enhancing disease resilience.

Language: Английский

Citations

0
Identification and validation of a major QTL, QFhb-6AL, for Fusarium head blight resistance on chromosome 6AL in wheat DOI

Zhenzhen Zhu,

Xinyuan Zhu, Na Zhang

et al.

Theoretical and Applied Genetics, Journal Year: 2025, Volume and Issue: 138(4)

Published: March 15, 2025

Language: Английский

Citations

0
Wheat resistance to Fusarium head blight and breeding strategies DOI Creative Commons
Haigang Ma, Yongjiang Liu, Suhong Zhang

et al.

Crop Health, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 8, 2025

Language: Английский

Citations

0
Cytogenetic identification and molecular mapping for the wheat-Thinopyrum ponticum introgression line with resistance to Fusarium head blight DOI
Xiaoying Yang, Xiaofang Cheng, Guangyi Wang

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(8)

Published: July 24, 2024

Language: Английский

Citations

2
Identification and transferring of a new Fusarium head blight resistance gene FhbRc2 from Roegneria ciliaris 3ScL chromosome arm into common wheat DOI Creative Commons
Y. X. Song, Danhua Zhang,

Yang Jingxin

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Fusarium head blight (FHB) threatens wheat production worldwide. Utilization of FHB resistant varieties is the most effective solution for disease control. Owing to limited sources resistance, mining novel resistance genes crucial. Here, we report an gene from a wild relative species, Roegneria ciliaris and developed germplasm containing this gene. Wheat-R. disomic addition line DA3Sc showed enhanced type II compared its sister 3Sc-Null without chromosome 3Sc, indicating that was contributed by 3Sc. The on 3Sc validated using F2 F2:3 populations derived cross between susceptible Aikang 58 (a cultivar), demonstrating lines with had significantly individuals This second identified in R. ciliaris, designated FhbRc2. To transfer FhbRc2 common wheat, produced double-monosomic population crossing Chinese Spring nulli-tetrasomic N3DT3B. Eight alien were genomic/fluorescence situ hybridization 3Sc-specific marker analysis. Only carrying long arm conferred further locating 3ScL. A compensating wheat-R. Robertsonian translocation T3DS·3ScL harboring provides potential genetic resource breeding resistance.

Language: Английский

Citations

2
Characterization of the wheat-tetraploid Thinopyrum elongatum 7E(7D) substitution line with Fusarium head blight resistance DOI Creative Commons
Dandan Wu, Fei Wang, Linfeng Chen

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 26, 2024

Fusarium head blight (FHB), a devastating disease of wheat production, is predominantly elicited by graminearum (Fg). The tetraploid Thinopyrum elongatum tertiary gene resource common that possesses high affinity and displays resistance traits against multiple biotic abiotic stress. We aim to employ utilize the novel FHB resources from wild germplasm for breeding. Durum wheat-tetraploid Th. amphiploid 8801 was hybridized with cultivars SM482 SM51, F5 generation generated. conducted cytogenetically in situ hybridization (ISH) technologies select confirm genetically stable 7E(7D) substitution line K17-1069-5, which showed expansion both field greenhouse infection experiments displayed no significant disadvantage agronomic compared their parents field. F2 segregation populations (K17-1069-5 × SM830) 7E chromosome conferred dominant dosage effect. developed 19 SSR molecular markers specific 7E, could be genetic mapping large breeding marker-assisted selection (MAS) during procedures future. isolated Fhb7 allele (Chr7E) using homology-based cloning, designated as TTE7E-Fhb7. In summary, our study K17-1069-5 contains resistance.

Language: Английский

Citations

1