Effects of Huang-Lian-Jie-Du decoction on improving skin barrier function and modulating T helper cell differentiation in 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis mice DOI Creative Commons

H. Zhang,

Q. Li,

Yaxing Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 20, 2024

Background: Atopic dermatitis (AD) is among the most frequently encountered skin diseases, bothering a considerable number of patients. Today, corticosteroids and antihistamines are numerous drugs applied for therapy AD. However, lengthy use them contributes to side effects, such as physiological changes in skin. As an alternative supplementary therapy, traditional Chinese medicine has become trend AD treatment. Huang-Lian-Jie-Du decoction (HLJDD), renowned herbal formula been employed treat inflammatory diseases its role regulating immunity remains unclear. The object this study was elucidate efficacy HLJDD reveal implicit mechanism from immunological perspective AD-like mice. Methods: In brief, 1-chloro-2,4-dinitrobenzene (DNCB) sensitization phase (1% DNCB) stimulation (1.5% were BALB/c dexamethasone (DXMS) administered orally Mice spleens collected evaluate HLJDD. 16S rRNA sequencing commensal microbiota fecal. vitro , spleen CD4 + T cells bone marrow-derived mast (BMMCs) co-cultured explore modulation helper (Th) phenotyping. Results: showcased substantial amelioration through upregulation FLG, LOR, AQP3, reducing scratching behaviors mice, Also, quantity infiltrated (MCs), pruritus-related mRNA decreased. addition, expression OX40/OX40L decreased by HLJDD, which critical Th-cell With treatment Th1/Th2 Th17/Treg ratios mice became balanced. structure affected could also improve imbalance . Conclusion: symptoms alleviating via Th2 expression. enhanced relative diversity changed intestinal microbiota. An in-depth found that balance ratio Th1/Th2, signaling pathway.

Language: Английский

Epidermal RORα Maintains Barrier Integrity and Prevents Allergic Inflammation by Regulating Late Differentiation and Lipid Metabolism DOI Open Access

Xiangmei Hua,

Maria K. Ficaro,

Nicole L. Wallace

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10698 - 10698

Published: Oct. 4, 2024

The skin epidermis provides a barrier that is imperative for preventing transepidermal water loss (TEWL) and protecting against environmental stimuli. underlying molecular mechanisms regulating functions sustaining its integrity remain unclear. RORα nuclear receptor highly expressed in the of normal skin. Clinical studies showed epidermal expression significantly reduced lesions multiple inflammatory diseases. In this study, we investigate central roles stabilizing function using mice with an epidermis-specific

Language: Английский

Citations

0
Effects of Huang-Lian-Jie-Du decoction on improving skin barrier function and modulating T helper cell differentiation in 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis mice DOI Creative Commons

H. Zhang,

Q. Li,

Yaxing Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 20, 2024

Background: Atopic dermatitis (AD) is among the most frequently encountered skin diseases, bothering a considerable number of patients. Today, corticosteroids and antihistamines are numerous drugs applied for therapy AD. However, lengthy use them contributes to side effects, such as physiological changes in skin. As an alternative supplementary therapy, traditional Chinese medicine has become trend AD treatment. Huang-Lian-Jie-Du decoction (HLJDD), renowned herbal formula been employed treat inflammatory diseases its role regulating immunity remains unclear. The object this study was elucidate efficacy HLJDD reveal implicit mechanism from immunological perspective AD-like mice. Methods: In brief, 1-chloro-2,4-dinitrobenzene (DNCB) sensitization phase (1% DNCB) stimulation (1.5% were BALB/c dexamethasone (DXMS) administered orally Mice spleens collected evaluate HLJDD. 16S rRNA sequencing commensal microbiota fecal. vitro , spleen CD4 + T cells bone marrow-derived mast (BMMCs) co-cultured explore modulation helper (Th) phenotyping. Results: showcased substantial amelioration through upregulation FLG, LOR, AQP3, reducing scratching behaviors mice, Also, quantity infiltrated (MCs), pruritus-related mRNA decreased. addition, expression OX40/OX40L decreased by HLJDD, which critical Th-cell With treatment Th1/Th2 Th17/Treg ratios mice became balanced. structure affected could also improve imbalance . Conclusion: symptoms alleviating via Th2 expression. enhanced relative diversity changed intestinal microbiota. An in-depth found that balance ratio Th1/Th2, signaling pathway.

Language: Английский

Citations

0