The freeze-avoiding mountain pine beetle (Dendroctonus ponderosae) survives prolonged exposure to stressful cold by mitigating ionoregulatory collapse DOI Creative Commons
Mads Kuhlmann Andersen, Amanda D. Roe,

Alice Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 18, 2024

Insect performance is intrinsically linked to environmental temperature, and surviving through winter represents a key challenge for temperate, alpine, polar species. To overwinter, insects have adapted wide range of strategies become truly cold hardy. However, while the physiological mechanisms underlying ability avoid or tolerate freezing been well-studied, little attention has given maintaining ion homeostasis at frigid temperatures in these species, despite this being central issue susceptible mild chilling. Here we investigate how prolonged exposure just above supercooling point affects balance freeze-avoiding larvae mountain pine beetle (Dendroctonus ponderosae) autumn, mid-winter, spring, relate it organismal recovery times survival outcomes. We found that hemolymph was gradually disrupted during first day exposure, characterized by hyperkalemia hyponatremia, after which plateau reached maintained rest seven experiment. The degree ionoregulatory collapse experienced correlated strongly with times, followed similar asymptotical progression. Mortality increased slightly most severe exposures, where K+ concentration highest, logistic relationship between hyperkalemia. Thus, tolerance D. ponderosae appears limited prevent manner less tolerant chill-susceptible insects, albeit much lower temperatures. Furthermore, posit prerequisite evolution insect freeze avoidance convergent ancestral maintain extreme stress.

Language: Английский

Finding the right thermal limit: a framework to reconcile ecological, physiological and methodological aspects of CTmax in ectotherms DOI Open Access
Michael Ørsted, Lisa Bjerregaard Jørgensen, Johannes Overgaard

et al.

Journal of Experimental Biology, Journal Year: 2022, Volume and Issue: 225(19)

Published: Oct. 1, 2022

Upper thermal limits (CTmax) are frequently used to parameterize the fundamental niche of ectothermic animals and infer biogeographical distribution under current future climate scenarios. However, there is considerable debate associated with methodological, ecological physiological definitions CTmax. The recent (re)introduction death time (TDT) model has reconciled some these issues now offers a solid mathematical foundation CTmax by considering both intensity duration stress. Nevertheless, origin boundaries this temperature-duration remain unexplored. Supported empirical data, we here outline reconciling framework that integrates TDT model, which operates at stressful temperatures, classic performance curve (TPC) typically describes biological functions permissive temperatures. Further, discuss how founded on balance between disruptive regenerative processes ultimately defines critical boundary temperature (Tc) separating TPC models. Collectively, allows inclusion repair accumulation heat stress, therefore also consistent conceptual approach understand impact high fluctuating conditions. improved experimental designs underpinnings consequences ectotherm tolerance.

Language: Английский

Citations

92
Insect resilience: unraveling responses and adaptations to cold temperatures DOI
Farman Ullah, Arzlan Abbas, Hina Gul

et al.

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(3), P. 1153 - 1169

Published: Feb. 22, 2024

Language: Английский

Citations

12
The underlying mechanisms of cold exposure-induced ischemic stroke DOI

Zhuangzhuang Chen,

Peilin Liu,

Xiaoshuang Xia

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 834, P. 155514 - 155514

Published: April 25, 2022

Language: Английский

Citations

33
A quantitative model of temperature-dependent diapause progression DOI Creative Commons
Loke von Schmalensee, Philip Süess, Kevin T. Roberts

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(36)

Published: Aug. 28, 2024

Winter diapause in insects is commonly terminated through cold exposure, which, like vernalization plants, prevents development before spring arrives. Currently, quantitative understanding of the temperature dependence termination limited, likely because phenotypes are generally cryptic to human eyes. We introduce a methodology tackle this challenge. By consecutively moving butterfly pupae species Pieris napi from several different conditions 20 °C, we show that proceeds as temperature-dependent rate process, with maximal rates at relatively temperatures and low warm extremely temperatures. Further, resulting thermal reaction norm can predict P. timing under variable Last, once , subsequent follows typical performance curve, around 31 °C minimum 2 °C. The sequence these thermally distinct processes (diapause postdiapause development) facilitates synchronous eclosion nature; microclimates where progresses quickly do not promote fast development, allowing individuals warmer winter catch up, vice versa. unveiling one process among others promotes parsimonious, quantitative, predictive model, wherein functions both an adaptation against premature during fall for synchrony spring.

Language: Английский

Citations

8
Acclimation temperature influences the thermal sensitivity of injury accumulation in Folsomia candida at extreme low and high temperatures DOI Creative Commons
Micha Wehrli, Stine Slotsbo, Jian Ge

et al.

Current Research in Insect Science, Journal Year: 2024, Volume and Issue: 6, P. 100089 - 100089

Published: Jan. 1, 2024

The importance of thermal acclimation for the Thermal Death Time (TDT) landscape common soil living springtail, Folsomia candida (Collembola, Isotomidae), was investigated. To this aim, we acclimated adult springtails at 10 °C (cold-acclimation) and 20 (warm-acclimation), respectively. In static tolerance assays, found relationship between survival exposure time a number stressful high low temperatures. Using logistic modelling, found, each temperature, until 50% mortality had been reached (Lt50). exponential functions TDT curves were by linear regression log10 Lt50 values against temperature. Results showed that cold significantly increased temperature dependence injury accumulation rate (increased slope 4 orders magnitude) in F. candida. Hence, changed status species from chill-susceptible to moderately chill-tolerant. cellular sub-zero temperatures not related freezing body water study. Congruently, significant negative effect on heat decreased sensitivity rate. Different slopes groups indicated shifted proportional mechanisms or nature mechanisms. Finally, compare combine extreme with previously published results longevity benign (from 0 30 °C) describe full niche

Language: Английский

Citations

5
Winter Is (Not) Coming: Is Climate Change Helping Drosophila suzukii Overwintering? DOI Creative Commons
Sara Sario, José Melo‐Ferreira, Conceição Santos

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 907 - 907

Published: June 25, 2023

Anthropogenic challenges, particularly climate change-associated factors, are strongly impacting the behavior, distribution, and survival of insects. Yet how these changes affect pests such as Drosophila suzukii, a cosmopolitan pest soft-skinned small fruits, remains poorly understood. This polyphagous is chill-susceptible, with cold temperatures causing multiple stresses, including desiccation starvation, also challenging immune system. Since invasion Europe United States America in 2009, it has been rapidly spreading to several European American countries (both North South American) African Asian countries. However, globalization global warming allowing an altitudinal latitudinal expansion species, thus colonization colder regions. review explores D. suzukii adapts survive during seasons. We focus on overwintering strategies behavioral adaptations migration or sheltering, seasonal polyphenism, reproductive adaptations, well metabolic transcriptomic response cold. Finally, we discuss continuation change may promote ability this species spread, what mitigation measures could be employed overcome cold-adapted suzukii.

Language: Английский

Citations

12
Interspecific differences in thermal tolerance landscape explain aphid community abundance under climate change DOI
Yuan-Jie Li, Siyang Chen, Lisa Bjerregaard Jørgensen

et al.

Journal of Thermal Biology, Journal Year: 2023, Volume and Issue: 114, P. 103583 - 103583

Published: May 1, 2023

Language: Английский

Citations

11
The freeze-avoiding mountain pine beetle (Dendroctonus ponderosae) survives prolonged exposure to stressful cold by mitigating ionoregulatory collapse DOI Creative Commons
Mads Kuhlmann Andersen, Amanda D. Roe,

Yuehong Liu

et al.

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(9)

Published: April 15, 2024

ABSTRACT Insect performance is linked to environmental temperature, and surviving through winter represents a key challenge for temperate, alpine polar species. To overwinter, insects have adapted range of strategies become truly cold hardy. However, although the mechanisms underlying ability avoid or tolerate freezing been well studied, little attention has given maintaining ion homeostasis at frigid temperatures in these species, despite this limiting tolerance susceptible mild chilling. Here, we investigated how prolonged exposure just above supercooling point affects balance freeze-avoidant mountain pine beetle (Dendroctonus ponderosae) larvae autumn, mid-winter spring, related it organismal recovery times survival. Hemolymph was gradually disrupted during first day exposure, characterized by hyperkalemia hyponatremia, after which plateau reached maintained rest 7-day experiment. The degree ionoregulatory collapse correlated strongly with times, followed similar asymptotical progression. Mortality increased slightly extensive exposures, where hemolymph K+ concentration highest, sigmoidal relationship found between survival hyperkalemia. Thus, freeze-avoiding D. ponderosae appears limited prevent manner that chill-susceptible insects, albeit much lower temperatures. Based on results, propose prerequisite evolution insect freeze avoidance may be convergent ancestral maintain extreme stress.

Language: Английский

Citations

4
Single-nuclei multiome ATAC and RNA sequencing reveals the molecular basis of thermal plasticity inDrosophila melanogasterembryos DOI Creative Commons
Thomas S. O’Leary, Emily E. Mikucki, Sumaetee Tangwancharoen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Embryogenesis is remarkably robust to temperature variability, yet there limited understanding of the homeostatic mechanisms that offset thermal effects during early development. Here, we measured acclimation response upper limits and profiled chromatin state transcriptome D. melanogaster embryos (Bownes Stage 11) using single-nuclei multiome ATAC RNA sequencing. We report acclimation, while preserving a common set primordial cell types, rapidly shifted limit. Cool-acclimated showed characterized by increased accessibility at transcription factor binding motifs for transcriptional activator Zelda, along with enhanced activity gene regulatory networks in types including foregut hindgut, mesoderm, peripheral nervous system. In addition, cool-acclimated had higher expression genes encoding ribosomal proteins enzymes involved oxidative phosphorylation. Despite hypothesis differential heat tolerance might be explained molecular chaperones, did not observe widespread differences or shock genes. Overall, our results suggest environmental robustness embryogenesis necessitates responses regulate speed development, potentially imposing metabolic costs constrain limits.

Language: Английский

Citations

0
Exploring the influence of habitat, ground refugia and behavioral strategies on the overwintering success of Drosophila suzukii, Matsumura DOI Open Access

Binita Shrestha,

Dara G. Stockton,

Stephen P. Hesler

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

The overwintering biology of Drosophila suzukii is poorly understood. While thermal tolerance studies suggest that winter morphotype females likely survive harsh winters given physiological changes lead to cold-hardening, additional factors such as habitat selection are important but In the present study, we conducted a field study at three vineyard sites investigate selection, semi-field measure survival outcomes depending on various ground refugia, and laboratory evaluate vertical movement in response simulated flood conditions often observed winter. Our results showed things. First, was most abundant wooded areas vineyards near pomace piles. Few were captured itself even fewer winery facilities. Second, insulating cover straw beneficial improving survivability. Third, while during stable periods below freezing, warm-up where snow melts subsequently floods ground, fly mobility critical for survival. Cold acclimated flies active participants their environment, freezing temperatures, navigating changing landscape features adjusting position temperature conditions. data first time cold capable temperatures water levels rise level thaw events. Thus, behavior ecology D. more complex than previously thought, there several addition hardening contribute success. © 2025 Society Chemical Industry.

Language: Английский

Citations

0