Identification and Characterization of Troponin T Associated with Development, Metabolism and Reproduction in Tribolium castaneum DOI Open Access
Wenzhuo Li,

Yaning Sun,

Yuanye Liang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2786 - 2786

Published: March 19, 2025

As a tropomyosin-binding component, troponin T (TnT) is essential for the Ca2+ regulation of striated muscles’ contraction and locomotion activity, but its impacts on growth development insects have rarely been reported. In this study, TnT was identified functionally characterized in Tribolium castaneum by RNA interference (RNAi) transcriptome analysis. The T. contained 1152 bp open reading frame encoding 383 amino acids. It displayed highest expression late pupae highly expressed integument CNS. Both larval early pupal injection dsTnT led to 100% cumulative mortality before pupal–adult transition. Late RNAi caused 26.01 ± 4.29% mortality; survivors successfully became adults, 49.71 6.51% died 10 days with dried shriveled abdomen, poorly developed reproductive system no offspring. Additionally, sequencing results indicated that key ecdysteroid juvenile hormone biosynthesis genes (CYP314A1, aldehyde dehydrogenase family 3 member B1 farnesol dehydrogenase) were affected, as well several cuticle protein, nutrition metabolism immune-related genes, suggesting may play prominent roles development, reproduction affecting these pathways. This study could provide brand-new target gene strategy pest control.

Language: Английский

Identification and Characterization of Troponin T Associated with Development, Metabolism and Reproduction in Tribolium castaneum DOI Open Access
Wenzhuo Li,

Yaning Sun,

Yuanye Liang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2786 - 2786

Published: March 19, 2025

As a tropomyosin-binding component, troponin T (TnT) is essential for the Ca2+ regulation of striated muscles’ contraction and locomotion activity, but its impacts on growth development insects have rarely been reported. In this study, TnT was identified functionally characterized in Tribolium castaneum by RNA interference (RNAi) transcriptome analysis. The T. contained 1152 bp open reading frame encoding 383 amino acids. It displayed highest expression late pupae highly expressed integument CNS. Both larval early pupal injection dsTnT led to 100% cumulative mortality before pupal–adult transition. Late RNAi caused 26.01 ± 4.29% mortality; survivors successfully became adults, 49.71 6.51% died 10 days with dried shriveled abdomen, poorly developed reproductive system no offspring. Additionally, sequencing results indicated that key ecdysteroid juvenile hormone biosynthesis genes (CYP314A1, aldehyde dehydrogenase family 3 member B1 farnesol dehydrogenase) were affected, as well several cuticle protein, nutrition metabolism immune-related genes, suggesting may play prominent roles development, reproduction affecting these pathways. This study could provide brand-new target gene strategy pest control.

Language: Английский

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