Three simple and cost-effective assays for AAC(6′)-Ib-cr enzyme activity

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

The enzyme AAC(6')-Ib-cr belongs to plasmid-mediated quinolone resistance (PMQR), first reported in 2006 and now widely disseminating. Here, we developed three phenotypic methods detect enzyme-producing Enterobacteriaceae (APE), two of which are proposed innovatively this research. These tests based on the following principles: (i) Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) can measure mass shift 42 Da resulting from ciprofloxacin acetylation by enzyme. (ii) Co-incubation disks with APE results inactivation drug activity, making it unable inhibit growth indicator organism. We named test method (QIM). (iii) Based principles modified Hodge test, designed (QHT). Through exploration optimal conditions for methods, found that MALDI-TOF MS provides most intuitive after 1 h incubation. interpretability QIM QHT was significantly improved when organism E. coli ATCC25922 replaced a quinolone-slightly-resistant isolate. However, Proteus mirabilis excluded both due its swarming motility. Next, validation study conducted using prospectively collected set 187 clinical strains, demonstrating 100% specificity (MSM: 141/141; QIM, QHT: 135/135) sensitivity 46/46; 33/33) compared genotype. In word, presented simple, efficient, cost-effective detecting APE, suitable microbiology laboratories under various prevention control hospital infections.

Language: Английский

Molecular characteristics of antimicrobial resistance and biofilm formation of bloodstream infection pathogens isolated from pediatric patients in Ukraine

IJID Regions, Journal Year: 2025, Volume and Issue: unknown, P. 100646 - 100646

Published: April 1, 2025

Language: Английский

Combining with matrine restores ciprofloxacin efficacy against qnrS producing E. coli in vitro and in vivo

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 198, P. 107132 - 107132

Published: Nov. 19, 2024

Language: Английский