New Zealand Journal of Botany,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 60
Published: Aug. 22, 2024
Ganoderma,
a
genus
deeply
rooted
in
traditional
medicinal
practices
across
diverse
Asian
cultures
throughout
history,
has
garnered
renewed
attention
due
to
its
promising
biotechnological
potential,
particularly
health-enhancing
properties.
The
distinctive
characteristics
exhibited
by
various
species
within
the
Ganoderma
render
them
invaluable
for
wide
spectrum
of
applications
both
industrial
and
pharmaceutical
contexts.
Recent
advancements
research
have
unveiled
plethora
captivating
opportunities
which
plays
pivotal
role.
These
encompass
range
applications,
including
but
not
limited
enzyme
production,
extraction
bioactive
compounds,
bioremediation,
bioprospecting,
biodegradation.
involvement
these
areas
highlights
versatility
significance
contributing
multiple
sectors.
This
comprehensive
review
explores
vistas
offering
insights
into
multifaceted
potential
impact
applications.
By
elucidating
biotechnology,
this
aims
underscore
role
contemporary
innovation.
Furthermore,
through
deeper
exploration
potentials
seeks
highlight
contributions
implications
future
biotechnology
sustainability.
Mycobiology,
Journal Year:
2025,
Volume and Issue:
53(1), P. 36 - 46
Published: Jan. 2, 2025
Laccases
are
enzymes
capable
of
oxidizing
phenolic
compounds
and
important
tools
in
different
industrial
processes.
Heterologous
expression
laccases
is
great
interest
biotechnological
applications
but
achieving
high
levels
challenging.
Three
have
been
identified
the
chestnut
blight
fungus
Cryphonectria
parasitica,
among
which
a
tannic
acid-inducible
laccase
(laccase3)
was
successfully
expressed
using
Saccharomyces
cerevisiae.
To
obtain
stable
fungal
laccases,
we
cloned
gene
encoding
an
extracellular
(Laccase1)
C.
parasitica
into
yeast
episomal
vector,
used
resulting
vectors
to
transform
S.
cerevisiae,
optimized
culture
conditions
selected
transformants
for
Laccase1
production.
We
also
tested
significance
signal
peptide
secretion
compared
it
with
widely
rice
amylase
peptide.
Among
four
constructs
full-length
containing
endogenous
peptide,
showed
highest
activity.
Interestingly,
stability
recombinant
vector
expressing
lower
than
that
mock
transformant,
suggesting
detrimental
effect
Laccase1-expressing
on
host
cells.
Thus,
produce
optimum
through
one-factor-at-a
-time
(OFAT)
were
2%
sucrose;
3%
nitrogen
base
without
amino
acid;
pH
5.0;
30
°C.
The
activity
found
be
2.2
U/mL
optimal
conditions,
6.5-fold
increase
conventional
medium.
Mycology: An International Journal on Fungal Biology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 17
Published: Sept. 20, 2024
Heavy
metal
contamination
has
severe
impacts
on
the
natural
environment.
The
currently
existing
physico-chemical
methods
have
certain
limitations,
restricting
their
wide-scale
application.
use
of
biological
agents
like
bacteria,
algae,
and
fungi
can
help
eliminate
heavy
metals
without
adversely
affecting
flora
fauna.
Due
to
inherent
ability
withstand
adverse
environmental
conditions,
nowadays,
mycoremediation
approaches
are
receiving
considerable
attention
for
removal
from
contaminated
sites.
In
this
review,
we
emphasised
role
white
rot
in
remediation
along
with
different
factors
influencing
biosorption,
effects
exposed
fungi,
mechanisms
involved.
Bibliometric
analysis
tools
been
applied
literature
search
trend
research
fungi-mediated
removal.
Annual
growth
rates
average
citations
per
document
5.08%
35.48,
respectively.
Processes,
Journal Year:
2024,
Volume and Issue:
12(7), P. 1341 - 1341
Published: June 27, 2024
The
growing
demand
in
industrial
and
biotechnological
settings
for
more
efficient
enzymes
with
enhanced
biochemical
features,
particularly
thermostability
thermotolerance,
necessitates
a
timely
response.
Renowned
their
versatility,
thermostable
offer
significant
promise
across
range
of
applications,
including
agricultural,
medicinal,
domains.
This
comprehensive
review
summarizes
the
structural
attributes,
catalytic
mechanisms,
connection
between
configuration
functional
activity
two
major
classes
enzymes:
α-amylases
laccases.
These
serve
as
valuable
models
understanding
foundation
behind
proteins.
By
highlighting
commercial
importance
interest
these
generate
among
researchers
further
optimization
innovation,
this
article
can
greatly
contribute
to
ongoing
research
on
aiding
industries
optimizing
production
processes
via
immobilization,
use
stabilizing
additives,
chemical
modification,
protein
engineering
(directed
evolution
mutagenesis),
genetic
(through
cloning
expression
genes).
It
also
gives
insights
exploration
suitable
strategies
factors
enhancing
like
increasing
substrate
affinity;
introducing
electrostatic,
intramolecular,
intermolecular
hydrophobic
interactions;
mitigating
steric
hindrance;
flexibility
an
active
site;
N-
C-terminal
engineering,
thus
resulting
heightened
multipronged
stability
notable
enhancements
enzymes’
applicability.
