Assessing amyloid fibrils and amorphous aggregates: A review DOI Creative Commons
Shaik Basha,

Darshan Chikkanayakanahalli Mukunda,

Aparna Pai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143725 - 143725

Published: May 1, 2025

Protein misfolding and aggregation play a central role in the progression of neurodegenerative diseases such as Alzheimer's Parkinson's. These aggregates manifest either structured amyloid fibrils enriched β-sheet conformations or irregular amorphous with diverse morphologies. Understanding their formation, structure, behavior is critical for deciphering disease mechanisms developing targeted diagnostics therapeutics. This review presents an integrated overview both conventional advanced techniques used to detect, distinguish, structurally characterize these protein aggregates. It covers range spectroscopic spectrometric tools, fluorescence, Raman, mass spectrometry that facilitate aggregate identification. Microscopy methods, including atomic force electron microscopy, are highlighted morphological analysis. The also discusses situ detection strategies using fluorescent dyes, conformation-specific antibodies, enzymatic reporters, real-time imaging. Separation methods like centrifugation, electrophoresis, chromatography outlined alongside structural analysis tools X-ray diffraction. Furthermore, growing utility computational approaches artificial intelligence predicting propensities integrating biological data emphasized. By critically evaluating each method's capabilities limitations, this provides practical forward-looking resource researchers studying complex landscape aggregation.

Language: Английский

Assessing amyloid fibrils and amorphous aggregates: A review DOI Creative Commons
Shaik Basha,

Darshan Chikkanayakanahalli Mukunda,

Aparna Pai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143725 - 143725

Published: May 1, 2025

Protein misfolding and aggregation play a central role in the progression of neurodegenerative diseases such as Alzheimer's Parkinson's. These aggregates manifest either structured amyloid fibrils enriched β-sheet conformations or irregular amorphous with diverse morphologies. Understanding their formation, structure, behavior is critical for deciphering disease mechanisms developing targeted diagnostics therapeutics. This review presents an integrated overview both conventional advanced techniques used to detect, distinguish, structurally characterize these protein aggregates. It covers range spectroscopic spectrometric tools, fluorescence, Raman, mass spectrometry that facilitate aggregate identification. Microscopy methods, including atomic force electron microscopy, are highlighted morphological analysis. The also discusses situ detection strategies using fluorescent dyes, conformation-specific antibodies, enzymatic reporters, real-time imaging. Separation methods like centrifugation, electrophoresis, chromatography outlined alongside structural analysis tools X-ray diffraction. Furthermore, growing utility computational approaches artificial intelligence predicting propensities integrating biological data emphasized. By critically evaluating each method's capabilities limitations, this provides practical forward-looking resource researchers studying complex landscape aggregation.

Language: Английский

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