Cartilage mechanobiology during limb growth DOI Open Access
Vineel Kondiboyina

Published: Jan. 1, 2024

The mechanobiology of cartilage during limb growth represents a complex interplay between mechanical forces and biological processes. However, the fundamental processes that involve formation from mesenchymal stem cells are not fully understood. Further, cellular level response in its native environment under physiological load is characterized. This thesis aims to bridge critical gaps our understanding development regeneration by investigating nuanced interactions mechanics within cartilaginous tissues. Identifying these research gaps, this encompasses three specific aims. Aim 1 focuses on characterizing viscoelastic material properties growing limbs, employing axolotls as an animal model. Our results reveal significant increases both instantaneous equilibrium shear moduli regeneration, coupled with notable changes short- long-term stress relaxation times. glycosaminoglycan content also development. 2 explores calcium signaling in-situ chondrocytes physiologically relevant cyclic loads dynamic hydrostatic pressure. findings underscore strain rate-dependent increase percentage responsive compressive loads, non-distinct time characteristics across loading conditions. Conversely, low magnitude pressure showed no impact chondrocytes. 3 investigates expression mechanosensitive ion channels (TRPV4, PIEZO1, PIEZO2) axolotl regeneration. study unveils presence TRPV4 PIEZO2 blastemal early late heightened condensing mesenchyme These taken together shed light intricate responses. implications abnormal mechanobiological profound, contributing developmental disorders musculoskeletal diseases. Understanding mechanisms conditions opens avenues for therapeutic strategies aimed at promoting proper mitigating skeletal abnormalities. Future will focus elucidating functional roles further expanding interconnections biology.--Author's abstract

Language: Английский

The salamander limb: a perfect model to understand imperfect integration during skeletal regeneration DOI Creative Commons
Camilo Riquelme‐Guzmán, Tatiana Sandoval‐Guzmán

Biology Open, Journal Year: 2024, Volume and Issue: 13(2)

Published: Feb. 6, 2024

ABSTRACT Limb regeneration in salamanders is achieved by a complex coordination of various biological processes and requires the proper integration new tissue with old. Among tissues found inside limb, skeleton most prominent component, which serves as scaffold provides support for locomotion animal. Throughout years, researchers have studied appendicular both after limb amputation result fracture healing. The final outcome has been widely seen faithful re-establishment skeletal elements, characterised seamless into mature tissue. process integration, however, not well understood, several works recently provided evidence commonly occurring flawed regenerates. In this Review, we take reader on journey through course bone formation salamanders, laying down foundation critically examining mechanisms behind integration. Integration phenomenon that could be influenced at steps regeneration, hence, assess current knowledge field discuss how early events, such histolysis patterning, influence skeleton.

Language: Английский

Citations

3
Cartilage mechanobiology during limb growth DOI Open Access
Vineel Kondiboyina

Published: Jan. 1, 2024

The mechanobiology of cartilage during limb growth represents a complex interplay between mechanical forces and biological processes. However, the fundamental processes that involve formation from mesenchymal stem cells are not fully understood. Further, cellular level response in its native environment under physiological load is characterized. This thesis aims to bridge critical gaps our understanding development regeneration by investigating nuanced interactions mechanics within cartilaginous tissues. Identifying these research gaps, this encompasses three specific aims. Aim 1 focuses on characterizing viscoelastic material properties growing limbs, employing axolotls as an animal model. Our results reveal significant increases both instantaneous equilibrium shear moduli regeneration, coupled with notable changes short- long-term stress relaxation times. glycosaminoglycan content also development. 2 explores calcium signaling in-situ chondrocytes physiologically relevant cyclic loads dynamic hydrostatic pressure. findings underscore strain rate-dependent increase percentage responsive compressive loads, non-distinct time characteristics across loading conditions. Conversely, low magnitude pressure showed no impact chondrocytes. 3 investigates expression mechanosensitive ion channels (TRPV4, PIEZO1, PIEZO2) axolotl regeneration. study unveils presence TRPV4 PIEZO2 blastemal early late heightened condensing mesenchyme These taken together shed light intricate responses. implications abnormal mechanobiological profound, contributing developmental disorders musculoskeletal diseases. Understanding mechanisms conditions opens avenues for therapeutic strategies aimed at promoting proper mitigating skeletal abnormalities. Future will focus elucidating functional roles further expanding interconnections biology.--Author's abstract

Language: Английский

Citations

0