Shared miRNA landscapes of COVID-19 and neurodegeneration confirm neuroinflammation as an important overlapping feature DOI Creative Commons
Sara Redenšek, David Vogrinc, Katja Goričar

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: March 17, 2023

Introduction Development and worsening of most common neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s multiple sclerosis, have been associated with COVID-19 However, the mechanisms neurological symptoms in patients sequelae are not clear. The interplay between gene expression metabolite production CNS is driven by miRNAs. These small non-coding molecules dysregulated diseases COVID-19. Methods We performed a thorough literature screening database mining to search for shared miRNA landscapes SARS-CoV-2 infection neurodegeneration. Differentially expressed miRNAs were searched using PubMed, while differentially five (Alzheimer’s Huntington’s amyotrophic lateral sclerosis) Human microRNA Disease Database. Target genes overlapping miRNAs, identified miRTarBase, used pathway enrichment analysis Kyoto Encyclopedia Genes Genomes Reactome. Results In total, 98 found. Additionally, two them (hsa-miR-34a hsa-miR-132) highlighted promising biomarkers neurodegeneration, they all hsa-miR-155 was upregulated four studies found be neurodegeneration processes well. Screening targets 746 unique strong evidence interaction. significant KEGG Reactome pathways being involved signaling, cancer, transcription infection. more specific confirmed neuroinflammation important feature. Discussion Our based approach has that may valuable potential prediction patients. can further explored drug or agents modify signaling pathways. Graphical Abstract Shared among investigated identified. hsa-miR-34a has-miR-132, present after Furthermore, together A analyses on list target finally top 20 evaluated their identification new targets. feature neuroinflammation. AD, disease; ALS, sclerosis; COVID-19, coronavirus disease 2019; HD, KEGG, Genomes; MS, PD, disease.

Language: Английский

Engineering extracellular vesicles by three‐dimensional dynamic culture of human mesenchymal stem cells DOI
Xuegang Yuan, Li Sun,

Richard Jeske

et al.

Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(6)

Published: June 1, 2022

Human mesenchymal stem cell (hMSC) derived extracellular vesicles (EVs) have shown therapeutic potential in recent studies. However, the corresponding components are largely unknown, and scale-up production of hMSC EVs is a major challenge for translational applications. In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion. Results demonstrate that activation endosomal sorting complexes required transport (ESCRT)-dependent -independent pathways. mRNA sequencing revealed global transcriptome alterations aggregates. Compared 2D-hMSC-EVs, quantity 3D-hMSC-EVs was enhanced significantly (by 2-fold), with smaller sizes, higher miR-21 miR-22 expression, an altered protein cargo (e.g., upregulation cytokines anti-inflammatory factors) uncovered by proteomics analysis, possibly due biogenesis. Functionally, rejuvenated senescent cells exhibited immunomodulatory potentials. summary, this study provides promising strategy scalable high-quality from potential.

Language: Английский

Citations

89
Hallmarks of sex bias in immuno-oncology: mechanisms and therapeutic implications DOI
Tong Xiao, Juyeun Lee, Timothy D. Gauntner

et al.

Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(5), P. 338 - 355

Published: April 8, 2024

Language: Английский

Citations

22
Small non-coding RNAs as important players, biomarkers and therapeutic targets in multiple sclerosis: A comprehensive overview DOI Creative Commons
Eliane Piket, Galina Yurevna Zheleznyakova, Lara Kular

et al.

Journal of Autoimmunity, Journal Year: 2019, Volume and Issue: 101, P. 17 - 25

Published: April 20, 2019

Multiple sclerosis (MS) is a leading cause of progressive disability among young adults caused by inflammation, demyelination and axonal loss in the central nervous system. Small non-coding RNAs (sncRNAs) are important regulators various biological processes could therefore play roles MS. Over past decade, large number studies investigated sncRNAs MS patients, focusing primarily on microRNAs (miRNAs). Overwhelming 500 miRNAs have been reported as dysregulated Nevertheless, owing to heterogeneity between it challenging evaluate reproducibility findings, turn hampering our knowledge about functional disease. We systematically searched main databases evaluated results from all that examined date (n = 61) provided detailed overview experimental design findings these studies. focused mechanisms most used predicted targets input for enrichment analysis highlight affected pathways. The prime pathway was TGF-β signaling. This multifunctional cytokine differentiation function T helper type 17 (Th17) regulatory (Treg) cells, with opposing functions Recent demonstrate importance controlling balance Th17/Th1 cells Tregs and, importantly, potential exploit this paradigm therapeutic purposes. Additionally, some discussed potentially serve biomarkers In order assist researchers evaluating evidence particular sncRNA pathogenesis MS, we provide date.

Language: Английский

Citations

76
Exosome Content–Mediated Signaling Pathways in Multiple Sclerosis DOI
Mina Mohammadinasr, Soheila Montazersaheb, Hormoz Ayromlou

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(8), P. 5404 - 5417

Published: Jan. 8, 2024

Language: Английский

Citations

12
Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis DOI Creative Commons
Liping Wang,

Chunyan Wang,

Xuqiang Jia

et al.

Cellular Physiology and Biochemistry, Journal Year: 2018, Volume and Issue: 50(5), P. 1754 - 1763

Published: Jan. 1, 2018

Background/Aims: A reduced prevalence of circulating regulatory T cells (Tregs)is a hallmark inflammatory rheumatoid arthritis (RA). However, the underlying mechanisms alterations Tregs are unclear. Methods: The ratio in peripheral blood healthy controls (HCs) and patients with RA was determined by flow cytometry. MicroRNA (miRNA) expression profiles exosomes derived from (RA-exosomes) those HCs (HC-exosomes) were detected microarray analysis, miR-17 measured quantitative real-time PCR. Transforming growth factor beta receptor II (TGFBR II) expressed interaction between TGFBR evaluated dual-luciferase reporter assay. Results: We found that RA-exosomes can selectively affect Treg differentiation vitro. Several miRNAs more abundant than HC-exosomes. Among upregulated RA, suppress induction inhibiting II. Conclusion: Our findings imply altered miRNA may contribute to pathogenesis disrupting homeostasis Tregs.

Language: Английский

Citations

74
Role of miR-155 in inflammatory autoimmune diseases: a comprehensive review DOI
Wang‐Dong Xu,

Si-Yu Feng,

An‐Fang Huang

et al.

Inflammation Research, Journal Year: 2022, Volume and Issue: 71(12), P. 1501 - 1517

Published: Oct. 29, 2022

Language: Английский

Citations

28
Multiplex Analysis of Cerebrospinal Fluid and Serum Exosomes MicroRNAs of Untreated Relapsing Remitting Multiple Sclerosis (RRMS) and Proposing Noninvasive Diagnostic Biomarkers DOI
Mina Mohammadinasr, Soheila Montazersaheb, Ommoleila Molavi

et al.

NeuroMolecular Medicine, Journal Year: 2023, Volume and Issue: 25(3), P. 402 - 414

Published: April 5, 2023

Language: Английский

Citations

18
Prevalence and Incidence of Multiple Sclerosis in Russian Federation: 30 Years of Studies DOI Creative Commons
Alexey Boyко, Mikhail Melnikov

Brain Sciences, Journal Year: 2020, Volume and Issue: 10(5), P. 305 - 305

Published: May 18, 2020

In the Russian Federation, multiple sclerosis prevalence rates vary from 10 to 80 cases per 100,000, depending on region and nationality of population. The main characteristics epidemiology in XX century this big territory are: (1) steady increase incidence rates, maybe because better diagnosis treatment, but also changes environmental/epigenetic risk profile and/or lifestyle factors; (2) female male ratio, mainly females; (3) appearance increasing frequency ethnic groups, previously free (Northern Tribes, Yakuts others). latest data show that European Russia, varies 30 cases, Siberia—from 20 70 with increases, especially women.

Language: Английский

Citations

48
Emerging Biomarkers of Multiple Sclerosis in the Blood and the CSF: A Focus on Neurofilaments and Therapeutic Considerations DOI Open Access
Tamás Biernacki, Zsófia Kokas, Dániel Sandi

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(6), P. 3383 - 3383

Published: March 21, 2022

Introduction: Multiple Sclerosis (MS) is the most common immune-mediated chronic neurodegenerative disease of central nervous system (CNS) affecting young people. This due to permanent disability, cognitive impairment, and enormous detrimental impact MS can exert on a patient’s health-related quality life. It great importance recognise it in time commence adequate treatment at an early stage. The currently used disease-modifying therapies (DMT) aim reduce activity thus halt disability development, which current clinical practice are monitored by imaging parameters but not biomarkers found blood and/or cerebrospinal fluid (CSF). Both radiological measures routinely monitor lack information fundamental pathophysiological features mechanisms MS. Furthermore, they lag behind process itself. By relapse becomes evident or new lesion appears MRI scan, potentially irreversible damage has already occurred CNS. In recent years, several that previously have been linked other neurological immunological diseases received increased attention Additionally, novel, potential with prognostic diagnostic properties detected CSF patients. Areas covered: this review, we summarise up-to-date knowledge research conducted known promising biomarker candidates Discussion: criteria relies three pillars: imaging, events, presence oligoclonal bands (which was reinstated into revision). Even though McDonald made diagnosis faster than prior iteration, still infallible toolset, especially very stage clinically isolated syndrome. Together gold standard measures, ancillary may just improve accuracy speed well become agents therapeutic efficacy make even more personalised reality near future. major disadvantage these past need obtain measure them. However, advances extremely sensitive immunoassays their measurement possible from peripheral when present only minuscule concentrations. should mark beginning utilisation era

Language: Английский

Citations

24
Let-7f-5p suppresses Th17 differentiation via targeting STAT3 in multiple sclerosis DOI Creative Commons
Zhihui Li, Yifei Wang, Dandan He

et al.

Aging, Journal Year: 2019, Volume and Issue: 11(13), P. 4463 - 4477

Published: July 15, 2019

T helper 17 (Th17) cells are regarded as key factors in the pathogenesis of multiple sclerosis (MS). Although involvement certain microRNAs (miRNAs) development MS has been reported, their roles Th17 cell differentiation and remain elusive. In this study, we identified that let-7f-5p expression is significantly downregulated CD4+ from patients during process differentiation. The overexpression suppressed differentiation, whereas knockdown enhanced progress. We then explored molecular mechanism through which signal transducer activator transcription 3 (STAT3), a pivotal factor cells, direct target let-7f-5p. contrast to let-7f-5p, STAT3 p-STAT3 protein levels were dramatically upregulated inversely correlated with peripheral blood patients. conclusion, functions potential inhibitor by targeting may serve new therapeutic target.

Language: Английский

Citations

37