UHPLC-QQQ-MS/MS method for the simultaneous quantification of 18 amino acids in various meats DOI Creative Commons
M.Z. Wang, Junxiu Guo, Huimin Lin

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 30, 2024

Amino acids are an essential source of human protein, and their content composition the main factors determining food protein utilization rate. Determining amino is in component analysis food. Therefore, a groundbreaking technique was developed utilizing ultra-high performance liquid chromatography interfaced with triple quadrupole mass spectrometer (UHPLC-QQQ-MS/MS) for concurrently quantifying 18 across various types meat. According to test results, it can be known that average glutamate (2.03 × 10

Language: Английский

Impact of liver-specific survival motor neuron (SMN) depletion on central nervous system and peripheral tissue pathology DOI Open Access
Monique Marylin Alves de Almeida, Yves De Repentigny, Sabrina Gagnon

et al.

Published: Feb. 10, 2025

Spinal muscular atrophy (SMA) is an inherited neuromuscular disorder stemming from deletions or mutations in the Survival Motor Neuron 1 ( SMN1 ) gene, leading to decreased levels of SMN protein, and subsequent motor neuron death muscle atrophy. While traditionally viewed as a predominantly affecting neurons, recent research suggests involvement various peripheral organs SMA pathology. Notably, liver has emerged significant focus due observed fatty phenotype dysfunction both mouse models patients. Despite these findings, it remains unclear whether intrinsic depletion protein contributes pathology central nervous systems. To address this knowledge gap, we developed model with liver-specific by utilizing Alb-Cre transgene together one Smn 2B allele exon 7 flanked loxP sites. Initially, evaluated phenotypic changes mice at postnatal day 19 (P19), time when severe SMA, 2B/- mice, typically exhibit many symptoms disease. Our findings indicate that does not induce death, atrophy, characteristics P19. However, mild steatosis was point, although no function were detected. pancreatic alterations resembled decrease insulin-producing β-cells increase glucagon-producing α-cells, accompanied reduction blood glucose plasma glucagon glucagon-like peptide (GLP-1) levels. Moreover, transient, P60 exhibited recovery function. mosaic pattern Cre-mediated excision precludes definitive conclusions regarding contribution overall tissue pathology, our highlight intricate connection between abnormalities adding nuanced layer understanding disease’s complexities.

Language: Английский

Citations

0
Impact of liver-specific survival motor neuron (SMN) depletion on central nervous system and peripheral tissue pathology DOI Creative Commons
Monique Marylin Alves de Almeida, Yves De Repentigny, Sabrina Gagnon

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 20, 2025

Spinal muscular atrophy (SMA) is caused by mutations in the Survival Motor Neuron 1 ( SMN1 ) gene. While traditionally viewed as a motor neuron disorder, there involvement of various peripheral organs SMA. Notably, fatty liver has been observed SMA mouse models and patients. Nevertheless, it remains unclear whether intrinsic depletion SMN protein contributes to pathology or central nervous systems. To address this, we developed model with liver-specific utilizing an Alb-Cre transgene together one Smn 2B allele Smn1 exon 7 flanked loxP sites. Initially, evaluated phenotypic changes these mice at postnatal day 19 (P19), when severe SMA, 2B/- mice, exhibit many symptoms disease. The does not induce death, neuromuscular muscle atrophy, characteristics typically P19. However, mild steatosis was observed, although no function were detected. pancreatic alterations resembled that decrease insulin-producing β-cells increase glucagon-producingα-cells, accompanied reduction blood glucose plasma glucagon glucagon-like peptide (GLP-1). These transient, P60 exhibited recovery function. mosaic pattern Cre-mediated excision precludes definitive conclusions regarding contribution overall tissue pathology, our findings highlight intricate connection between abnormalities

Language: Английский

Citations

0
Experimental and interaction mechanism with novel depressant lysine for the removal of copper ions activation on sphalerite surface DOI

Lingyun Bao,

Wengang Liu, Wenbao Liu

et al.

Minerals Engineering, Journal Year: 2025, Volume and Issue: 226, P. 109247 - 109247

Published: March 14, 2025

Language: Английский

Citations

0
Multicomponent Metabiotic Actoflor-S Has Therapeutic Potential for Bowel, Liver, and Metabolic Disorders DOI
Timur Vakhitov, С. И. Ситкин

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

One of the aspects bacterial symbiosis with host is exchange metabolites. Stimulation growth beneficial bacteria in microbiota using metabiotic Actoflor®-S led to a change composition flow its Analysis blood metabolome mice by GC-MS showed that animals receiving had an increased concentration almost all amino acids and decreased most carboxylic their derivatives, including lactic acid. Comparison obtained results literature data action leads normalization at least 10 marker compounds, whose was significantly changed patients ulcerative colitis. The property normalize turned out be inherent only entire set components, but not parts, which indicator emergent properties metabiotic. Overall, work also indicate metabolites are important participants maintaining health; often, they have individual activity, cases, activity consequence combined action.

Language: Английский

Citations

0
Alterations in hepatic amino acid metabolism related to MASLD in individuals with obesity DOI
Armando Jesús Pérez-Díaz,

Inmaculada Ros‐Madrid,

María Antonia Martínez‐Sánchez

et al.

Journal of Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

Citations

0
Metabolomics at the cutting edge of risk prediction of MASLD DOI Creative Commons

En Ying Tan,

Mark Muthiah, Arun J. Sanyal

et al.

Cell Reports Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 101853 - 101853

Published: Dec. 1, 2024

Language: Английский

Citations

2
Impact of liver specific survival motor neuron (SMN) depletion on peripheral and central nervous system tissue pathology DOI Creative Commons
Monique Marylin Alves de Almeida, Yves De Repentigny, Sabrina Gagnon

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Sept. 24, 2024

Spinal muscular atrophy (SMA) is caused by mutations in the Survival Motor Neuron 1 (SMN1) gene. While traditionally viewed as a motor neuron disorder, there involvement of various peripheral organs SMA. Notably, fatty liver has been observed SMA mouse models and patients. Nevertheless, it remains unclear whether intrinsic depletion SMN protein contributes to pathology or central nervous systems. To address this, we developed model with liver-specific utilizing an Alb-Cre transgene together one Smn2B allele Smn1 exon 7 flanked loxP sites. Initially, evaluated phenotypic changes these mice at postnatal day 19 (P19), when severe SMA, Smn2B/- mice, exhibit many symptoms disease. The does not induce death, neuromuscular muscle atrophy, characteristics typically P19. However, mild steatosis was observed, although no function were detected. pancreatic alterations resembled that Smn2B/-mice, decrease insulin-producing β-cells increase glucagon-producingα-cells, accompanied reduction blood glucose plasma glucagon glucagon-like peptide (GLP-1). These transient, P60 exhibited recovery function. mosaic pattern Cre-mediated excision precludes definitive conclusions regarding contribution overall tissue pathology, our findings highlight intricate connection between abnormalities

Language: Английский

Citations

1
The toxicity of cisplatin derives from effects on renal organic ion transporters expression and serum endogenous substance levels DOI
Mingkang Zhang,

Yile Li,

Yanrong Ma

et al.

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 192, P. 114949 - 114949

Published: Aug. 24, 2024

Language: Английский

Citations

0
UHPLC-QQQ-MS/MS method for the simultaneous quantification of 18 amino acids in various meats DOI Creative Commons
M.Z. Wang, Junxiu Guo, Huimin Lin

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 30, 2024

Amino acids are an essential source of human protein, and their content composition the main factors determining food protein utilization rate. Determining amino is in component analysis food. Therefore, a groundbreaking technique was developed utilizing ultra-high performance liquid chromatography interfaced with triple quadrupole mass spectrometer (UHPLC-QQQ-MS/MS) for concurrently quantifying 18 across various types meat. According to test results, it can be known that average glutamate (2.03 × 10

Language: Английский

Citations

0