Live and Dead Clostridium butyricumGKB7 Diminish Osteoarthritis Pain and Progression in Preclinical Animal Model DOI

Li‐Chai Chen,

Yen‐You Lin,

You‐Shan Tsai

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(11), P. 4927 - 4935

Published: June 25, 2024

ABSTRACT Osteoarthritis (OA) is a degenerative joint disease primarily affecting the elderly. It characterized by progressive decline of cartilage and alterations in underlying bone. Several probiotic strains have exhibited immunomodulatory anti‐inflammatory properties. Here, we examined functions live dead Clostridium butyricum GKB7 (GKB7‐L GKB7‐D) preclinical anterior cruciate ligament transection (ACLT)‐enhanced OA procedure. Oral administration GKB7‐L GKB7‐D ameliorated ACLT‐induced bone pain as assessed weight‐bearing behavioral testing but did not affect body weight. Micro‐computed tomography (CT) results showed that diminished destruction loss. GKB7‐D‐enriched therapies also reduced production pro‐inflammatory cytokines interleukin (IL)‐1β tumor necrosis factor (TNF)‐α, well chondrolytic matrix metalloproteinase (MMP)‐3, leading to inhibition aggrecan collagen type II degradation thereby blocking breakdown. We therefore suggest oral supplementation with or can be beneficial prevention treatment OA.

Language: Английский

LATS1-modulated ZBTB20 perturbing cartilage matrix homeostasis contributes to early-stage osteoarthritis DOI Creative Commons

Xue Hao,

Jing Zhao,

Liyuan Jia

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: March 12, 2025

Abstract Osteoarthritis (OA) is one of the most common degenerative joint diseases in elderly, increasing prevalence and posing a substantial socioeconomic challenge, while no disease-modifying treatments available. Better understanding early molecular events will benefit early-stage diagnosis clinical therapy. Here, we observed nucleus accumulation ZBTB20, member ZBTB-protein family, chondrocytes OA. Chondrocytes-specific depletion Zbtb20 adult mice attenuated DMM-induced OA progress, restored balance extracellular matrix anabolism catabolism. The NF-κB signaling mediated disturbance ECM maintenance by ZBTB20 requires its suppression Pten consequent PI3K-Akt activation. Furthermore, subcellular localization was modulated kinase LATS1. Independent approaches to modulating via utilizing TRULI DAPA can restore homeostasis, improving abnormal behavior moderating cartilage degeneration. compounds may serve as anti-OA drugs.

Language: Английский

Citations

1
Research progress on macrophage polarization during osteoarthritis disease progression: a review DOI Creative Commons

Xiangzhi Yin,

Quan Wang, Yijie Tang

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2024, Volume and Issue: 19(1)

Published: Sept. 28, 2024

Language: Английский

Citations

5
Do cytokines play a role in the transition from acute to chronic musculoskeletal pain? DOI Creative Commons

Ebersberger Andrea,

Hans‐Georg Schaible

Pharmacological Research, Journal Year: 2025, Volume and Issue: 212, P. 107585 - 107585

Published: Jan. 7, 2025

Musculoskeletal pain has a high prevalence of transition to chronic and/or persistence as for years or even lifetime. Possible mechanisms the development such states are often reflected in inflammatory neuropathic processes involving, among others, cytokines and other molecules. Since biologics blockers TNF IL-6 can attenuate inflammation subset patients with rheumatoid arthritis, question arises what extent involved generation human musculoskeletal diseases. In numerous experimental non-human studies, have been shown alter neuronal sensitivity peripheral central nociceptive systems. this review, we addressed involvement postoperative pain, complex regional syndrome, osteoarthritis, temporomandibular joint disease, low back fibromyalgia using PubMed searches including meta-analyses data. There is evidence that certain pro- anti-inflammatory regulated all these diseases, both acute disease states. However, within data, found great deal heterogeneity association between cytokine levels pain. Neutralization showed antinociceptive effects subgroups (e.g., proportion arthritis), but failed reduce diseases osteoarthritis). More systematic studies needed unravel pathogenic role taking into account process initiation maintenance.

Language: Английский

Citations

0
Targeting the NLRP3 inflammasome as a novel therapeutic target for osteoarthritis DOI

Varnita Karmakar,

Mayukh Chain,

Ankit Majie

et al.

Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

Citations

0
The inflammatory endotype in osteoarthritis: Reflections from the 2024 OARSI Clinical Trials Symposium (CTS) with a special emphasis on feasibility for clinical development DOI Creative Commons
M.A. Karsdal, Lucio C. Rovati, J. Tambiah

et al.

Osteoarthritis and Cartilage Open, Journal Year: 2025, Volume and Issue: unknown, P. 100572 - 100572

Published: Jan. 1, 2025

Language: Английский

Citations

0
Association between serum high-sensitivity C-reactive protein levels and osteoarthritis in adults from NHANES 2015 to 2018 DOI Creative Commons
Tao Gao,

Zhiyu Chen,

Tao Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 15, 2025

To explore the association between serum high-sensitivity C-reactive protein (HSCRP) levels and osteoarthritis (OA) in adults, providing new evidence for diagnosis of adult OA. We selected data from 2015–2018 National Health Nutrition Examination Survey (NHANES) conducted a cross-sectional study. Serum HSCRP were extracted laboratory data, OA patients identified through questionnaire responses. Participants under age 20 those with incomplete excluded. used multivariable logistic regression models, restricted cubic spline (RCS) functions, stratified analyses to study adults. After screening, total 9,948 participants included, among whom 1,196 patients, representing prevalence rate 12.02%. Multivariable analysis, along three adjusted showed positive correlation occurrence Compared lowest quartile, highest quartile 1.86-fold higher (95% confidence interval: 1.55 ~ 2.23, P < 0.001). The analysis significant increase incidence rising (P 0.05). Subgroup forest plot indicated across different subgroups, such as age, gender, hypertension status, activity drinking Smoke status There is When patient's level elevated, possibility should be considered.

Language: Английский

Citations

0
Understanding the molecular basis of NO-releasing β-cyclodextrin derivatives and ferulic acid co-delivery for advanced therapeutics: A theoretical study DOI Creative Commons
Ji Mu, Minghong Ni,

Wei Dong

et al.

Carbohydrate Polymer Technologies and Applications, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: March 1, 2025

Language: Английский

Citations

0
Inverse L-shaped association of triglyceride glucose index with all-cause and cerebral cardiovascular-related mortality in osteoarthritis patients: a cohort of 4145 patients DOI
Zitian Zheng, Meng Yang, Zhiyu Zhang

et al.

Clinical Rheumatology, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

0
Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis DOI Open Access

Leyre Brizuela,

René Buchet, Carole Bougault

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2896 - 2896

Published: March 22, 2025

Links between cathepsin K and the pathophysiology of osteoarthritis (OA) can be established, not least because overabundance in serum OA patients upregulation degraded cartilage animal models OA. Chondrocytes, chondroclasts, or osteoclasts contribute to accumulated at diseased osteochondral junction. After a general presentation physiology, as well its degradation processes, we describe function effect on via type II collagen cleavage. An overview most promising inhibitors is then presented, together with their vitro effects. Although intensive research initially focused bone resorption, there growing interest potential these drugs prevent degradation. In this review, summarize pre-clinical clinical trials that support use treatment To date, no molecules are commercially available, although few have undergone trials, but believe development could broaden therapeutic arsenal for

Language: Английский

Citations

0
Engineered mesenchymal stromal cells with interleukin-1beta sticky-trap attenuate osteoarthritis in knee joints DOI Creative Commons
Chris Kim,

Biao Li,

Sayaka Nakamura

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 8, 2025

Osteoarthritis (OA) is a common chronic inflammatory joint disease, in which innate immunity plays pivotal role pathogenesis. Anti-interleukin-1(IL-1) therapies have shown inconsistent results clinical trials, potentially due to mismatch the spatial and temporal dynamics of interleukin-1beta (IL-1β) production therapeutic interventions. To address this issue, we developed novel IL-1β "sticky-trap" utilizing cell gene-based technologies from our lab evaluated its efficacy reducing osteoarthritis progression using murine destabilization medial meniscus (DMM) OA model compact bone-derived mesenchymal stromal (MSC)-based gene expression system. The extracellular domain interleukin-1 receptor 2 (IL1R2) was employed design sticky IL1R2 trap (stkIL1R2). A MSC line engineered for delivery. Although stkIL1R2 undetectable supernatants by enzyme-linked immunosorbent assay (ELISA) Western blot, it localized on surface matrix (ECM) demonstrated specific binding fluorescent protein-fused assay. Doxycycline (Dox)-induced significantly inhibited lipocalin-2 (LCN2) biomarker activity. For vivo experiments, 5 × 104 Dox-inducible stkIL1R2f expressing MSCs were injected into knee joints DMM mice. Bioluminescence imaging revealed survival up 7 weeks post-injection. Histological analyses at 10 post-injection, including Safranin-O Masson trichrome staining, showed that treated exhibited less cartilage degradation synovitis compared controls, as assessed Research Society International (OARSI) scoring femur, tibia, synovium. Moreover, treatment reduced metalloproteinases-13 (MMP-13) positive cells collagen type II affected joints. In conclusion, an inducible IL1 sticky-trap, ECM specifically bound IL-1β. These survived normal delayed inflammation model. This study introduces promising approach combat progression.

Language: Английский

Citations

0