Identifying autophagy-related mRNAs and potential ceRNA networks in meniscus degeneration based on RNA sequencing and experimental validation DOI Creative Commons
Jun Zhang,

Jiayong Zhu,

Xinyu Zou

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e32782 - e32782

Published: June 1, 2024

PurposeThe intimate connection between long noncoding RNA (lncRNA) and autophagy has been established in cartilage degeneration. However, their roles meniscal degeneration remain ambiguous. This study aimed to identify the key autophagy-related lncRNA its associated regulatory network context of osteoarthritis (OA).MethodsRNA sequencing was performed differentially expressed lncRNAs (DELs) mRNAs (DEMs), which were then conducted enrichment analyses using DAVID database Metascape. Autophagy-related DEMs identified by combining with data from Human Autophagy Database. Three databases used predict miRNA, DIANA LncBase Predicted utilized miRNA-lncRNA interactions. Based on these predictions, comprehensive competitive endogenous (ceRNA) constructed. The expression levels classical markers ceRNA validated. Additionally, Gene Set Enrichment Analysis (GSEA) DEMs.Results310 DELs 320 identified, five upregulated one downregulated DEMs. Through reverse prediction paired interactions, verification RT-qPCR, two (PCAT19, CLIP1-AS1), miRNA (has-miR-3680-3p has-miR-4795-3p) (BAG3 HSP90AB1) included constructed networks. GSEA indicated that increased inhibited glycosaminoglycan biosynthesis degenerative meniscus.ConclusionThis presented first construction involving lncRNA-miRNA-mRNA interactions OA meniscus. These findings offered valuable insights into mechanisms underlying provided potential targets for therapeutic intervention.

Language: Английский

Foot osteoarthritis research: a bibliometric analysis DOI Creative Commons
Hylton B. Menz, Shannon E. Munteanu, Kade L. Paterson

et al.

Osteoarthritis and Cartilage, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Prevalence and Factors Associated with High Impact Chronic Pain in Knee Osteoarthritis: the Johnston County Health Study DOI Creative Commons
Steven Z. George, Kelli D. Allen, Carolina Álvarez

et al.

Journal of Pain, Journal Year: 2024, Volume and Issue: unknown, P. 104687 - 104687

Published: Sept. 1, 2024

Language: Английский

Citations

2
Incidence and Progression of Foot Osteoarthritis in a Longitudinal Cohort: The Johnston County Osteoarthritis Project DOI

Rami Eltaraboulsi,

Amanda E. Nelson, Carolina Álvarez

et al.

Gerontology, Journal Year: 2024, Volume and Issue: 70(10), P. 1013 - 1022

Published: June 22, 2024

Introduction: The aim of this study was to examine the incidence and progression foot osteoarthritis (OA), as well associated factors, in a community-based cohort. Methods: Baseline (2013–2015) follow-up (2016–2018) radiographs were available for 541 participants (71% women, mean age 69 years; 35% black, 53% with obesity). LaTrobe Foot Atlas used osteophytes (OPs, score 0–3) joint space narrowing (JSN, at 5 sites. Incident radiographic OA (rOA) baseline <2 OP JSN all joints ≥2 or any joints. Progression worsening rOA. At follow-up, reported presence/absence symptoms completed Ankle Outcome Score (FAOS) each foot. Joint-based logistic regression models generalized estimating equations associations (adjusted odds ratio [aOR], 95% confidence interval [CI]) rOA covariates. Results: Among 928 feet without rOA, 4% developed incident (2% those symptoms). 154 55% had (16% Women higher body mass index (BMI) more likely have (aOR [95% CI] = 4.10 [1.22, 13.8] 1.60 [1.31, 1.97], respectively); history gout (2.75 [1.24, 6.07]). BMI worse scores on FAOS subscales (aORs range 1.21–1.40). Conclusion: is common but not necessarily related symptoms. may be modifiable risk factor OA.

Language: Английский

Citations

1
Identifying autophagy-related mRNAs and potential ceRNA networks in meniscus degeneration based on RNA sequencing and experimental validation DOI Creative Commons
Jun Zhang,

Jiayong Zhu,

Xinyu Zou

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e32782 - e32782

Published: June 1, 2024

PurposeThe intimate connection between long noncoding RNA (lncRNA) and autophagy has been established in cartilage degeneration. However, their roles meniscal degeneration remain ambiguous. This study aimed to identify the key autophagy-related lncRNA its associated regulatory network context of osteoarthritis (OA).MethodsRNA sequencing was performed differentially expressed lncRNAs (DELs) mRNAs (DEMs), which were then conducted enrichment analyses using DAVID database Metascape. Autophagy-related DEMs identified by combining with data from Human Autophagy Database. Three databases used predict miRNA, DIANA LncBase Predicted utilized miRNA-lncRNA interactions. Based on these predictions, comprehensive competitive endogenous (ceRNA) constructed. The expression levels classical markers ceRNA validated. Additionally, Gene Set Enrichment Analysis (GSEA) DEMs.Results310 DELs 320 identified, five upregulated one downregulated DEMs. Through reverse prediction paired interactions, verification RT-qPCR, two (PCAT19, CLIP1-AS1), miRNA (has-miR-3680-3p has-miR-4795-3p) (BAG3 HSP90AB1) included constructed networks. GSEA indicated that increased inhibited glycosaminoglycan biosynthesis degenerative meniscus.ConclusionThis presented first construction involving lncRNA-miRNA-mRNA interactions OA meniscus. These findings offered valuable insights into mechanisms underlying provided potential targets for therapeutic intervention.

Language: Английский

Citations

0