Royal Society of Chemistry eBooks,
Journal Year:
2024,
Volume and Issue:
unknown, P. 95 - 120
Published: Dec. 20, 2024
Fluorometric
sensors
and
devices
are
important
tools
for
the
detection
measurement
of
biomarkers,
which
indicators
biological
processes
diseases.
These
involve
emission
light
from
fluorescent
molecules
upon
interaction
with
target
biomolecules,
resulting
in
a
measurable
signal.
Fluorescence-based
techniques,
instance,
fluorescence
microscopy,
fluorometry,
resonance
energy
transfer
(FRET),
probes,
enable
researchers
to
study
high
sensitivity
specificity.
offer
numerous
benefits
over
conventional
methods,
like
higher
sensitivity,
selectivity,
versatility,
ability
sense
various
biomarkers
instantaneously.
In
this
chapter,
we
provide
an
overview
recent
developments
fluorometric
biomarker
detection,
including
design
principles,
sensing
mechanisms,
fabrication
techniques
developing
sensors.
The
challenges
future
directions
technology
also
discussed.
This
chapter
highlights
importance
these
advancing
our
understanding
systems
their
potential
improving
healthcare,
food
safety,
environmental
monitoring.
Journal of Separation Science,
Journal Year:
2024,
Volume and Issue:
47(14)
Published: July 1, 2024
Furosemide
(FUR),
banned
in
sports
events
by
the
World
Anti‐Doping
Agency,
is
a
key
target
drug
tests,
necessitating
pretreatment
material
capable
of
selectively,
rapidly,
and
sufficiently
separating/enriching
analytes
from
complex
matrices.
Herein,
metal‐mediated
magnetic
molecularly
imprinted
polymer
(mMIP)
was
rationally
designed
synthesized
for
specific
capture
FUR.
The
preparations
involved
utilization
chromium
(III)
as
binding
pivot,
(3‐aminopropyl)triethoxysilane
functional
monomer,
Fe
3
O
4
core,
all
assembled
via
free
radical
polymerization.
Both
morphologies
adsorptive
properties
mMIP
were
characterized
using
multiple
methods.
resulting
Cr(III)‐mediated
(ChM‐mMIP)
presented
excellent
selectivity
specificity
toward
Under
optimized
conditions,
adsorption
capacity
reached
128.50
mg/g
within
10
min,
imprinting
factor
10.41.
Moreover,
it
also
successfully
applied
dispersive
solid‐phase
extraction
material,
enabling
detection
FUR
concentration
low
20
ng/mL
human
urine
samples
when
coupled
with
high‐performance
liquid
chromatography/photodiode
array.
Overall,
this
study
offers
valuable
strategy
development
novel
recognition
material.
Bulletin of the National Research Centre/Bulletin of the National Research Center,
Journal Year:
2024,
Volume and Issue:
48(1)
Published: March 22, 2024
Abstract
Background
Catalase
is
an
important
enzyme
that
helps
protect
cells
against
oxidative
stress.
The
current
protocol
presents
a
reliable
method
for
measuring
catalase
(CAT)
activity
in
biological
systems
using
the
CUPRAC-CAT
method.
Methods
In
method,
component
of
enzymatic
reaction
was
incubated
before
adding
Cu(Nc)
2
2+
reagent.
unreacted
substrates
reduced
Cu(II)-the
neocuproine
complex,
resulting
highly
colored
Cu(I)-neocuproine
product,
which
could
be
detected
spectrophotometrically
at
450
nm.
negative
correlation
between
and
absorbance
complex
examined.
To
assess
optimization
production,
response
surface
methodology
(RSM)
employed,
specifically
utilizing
Box–Behnken
design
(BBD).
Additionally,
reliability
newly
developed
confirmed
through
Bland–Altman
analysis
paired
samples,
employing
peroxovanadate
Results
novel
just
as
accurate
established
standard;
two
methods
0.99.
stable,
sensitive,
linear,
reproducible,
accurate,
selective
can
used
quantifying
stress
while
liver
tissue
homogenates.
Conclusions
This
study
has
demonstrated
straightforward
dependable
evaluating
activity.
free
from
interference
easily
employed
scientific
research,
ensuring
high
level
accuracy
precision.
effective
technique
to
monitor
bacterial
contamination.
provides
quick
results
help
ensure
food
safety
prevent
or
address
Graphical
abstract
Sensors,
Journal Year:
2024,
Volume and Issue:
24(21), P. 7068 - 7068
Published: Nov. 2, 2024
Currently,
analyzing
pharmaceuticals
and
biomarkers
is
crucial
for
ensuring
medication
safety
protecting
life
health,
there
an
urgent
need
to
develop
new
efficient
analytical
techniques
in
view
of
the
limitations
traditional
methods.
Molecularly
imprinted
ratiometric
fluorescent
(MI-RFL)
sensors
have
received
increasing
attention
field
detection
due
their
high
selectivity,
sensitivity
anti-interference
ability,
short
response
time,
visualization.
This
review
summarizes
recent
advances
MI-RFL
detection.
Firstly,
fluorescence
sources
working
mechanisms
are
briefly
introduced.
On
this
basis,
strategies
preparing
molecularly
polymers,
such
as
dummy
template
imprinting,
nanoimprinting,
multi-template
stimulus-responsive
imprinting
strategies,
presented.
Then,
dual-
triple-emission
types
Subsequently,
specific
applications
pharmaceutical
analysis
highlighted.
In
addition,
innovative
point-of-care
testing
discussed
in-depth.
Finally,
challenges
proposed,
research
outlook
development
trends
prospected.
