An isocratic HPLC-UV analytical procedure for assessment of glutathione and its related substances

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 249, P. 116374 - 116374

Published: July 20, 2024

Reduced glutathione (GSH) is an endogenous tripeptide antioxidant which plays a crucial role in variety of physiological and pathological activities. Although GSH not present any FDA-approved drug product, dietary supplement products compounded drugs are available to patients the US. Several incidents toxicity have occurred recent years due endotoxin or otherwise contaminated drugs. Efficient sensitive analytical methods needed for assessing ensuring quality substance associated products. Impurities A (L-cysteinylglycine), B (cysteine), C (oxidized L-glutathione) D (γ-L-glutamyl-L-cysteine) main related impurities been detected quantified by capillary electrophoresis qNMR procedures. However, there no reported HPLC detecting quantifying three A, even though numerous analyzing impurity C. In this report, isocratic HPLC-UV procedure was developed validated separating identifying A-D as well newly identified degradant, L-pyroglutamic acid (pGlu), within 10 minutes with resolution (R

Language: Английский

A rapid and high-throughput fraud detection method of Philippine coconut wine ( lambanog ) using ¹ H qNMR spectroscopy

Food Additives & Contaminants Part A, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 10

Published: Dec. 9, 2024

Methanol contamination of the Philippine coconut spirit

Language: Английский