DNA Delivery to Intact Plant Cells by Casein Nanoparticles with Confirmed Gene Expression

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(16)

Published: Feb. 19, 2024

Abstract This research presents gene expression after DNA delivery into intact plant cells by protein nanoparticles. The is carried out casein nanoparticles (CNPs). A plasmid harboring the red fluorescent DsRed sequence absorbed to CNPs surface electrostatic interaction and served as a model in this study, its monitored fluorescence of protein. zeta potential tuned altering pH obtain sufficient between for successful Nicotiana benthamiana . are covalently modified with green dye 6‐Aminofluorescein (6‐AF) determine their location plant. To assess ability deliver cells, CNP/6‐AF/DsRed conjugates infiltrated N. leaves. Confocal microscopy results showed intracellular nucleus uptake at 4.5 concentration 2 mg mL −1 CNPs: ratio 1:0.01. confirmed RT‐PCR qRT‐PCR. first appearance emitted signal observed 24 h post‐infiltration.

Language: Английский

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Effect of ionic strength on DNA–dye interactions of Victoria blue B and methylene green using UV–visible spectroscopy

Zeitschrift für Physikalische Chemie, Journal Year: 2023, Volume and Issue: 238(1), P. 173 - 186

Published: Nov. 17, 2023

Victoria blue and methylene green dyes have both been extensively studied due to their numerous applications, including ability bind DNA. Dyes are very important in everyday life with applications textile, cosmetics, food pharmaceutical industries. It has found that some of them adversely affect human health causing severe abnormalities. Among these abnormalities, cancer is great concern its fatal almost non-recoverable nature. In this work we the binding two namely B (VBB) Methylene (MG) double stranded DNA (Salmon sperm). The interactions were presence different concentrations buffer solutions at a constant pH. selected showed double-stranded through intercalation electrostatic modes. Upon increasing ionic strength (K b ) value for MG was decreased whereas increased VBB, which conclude that, higher (0.5 M) DNA–MG lower DNA–VVB maximum. carcinogenicity given dye indicated from constants current study. Based on recorded K values it concluded proper disposing precautions should be taken while utilizing/dealing order minimize/avoid impact environment health.

Language: Английский

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Shaking Up the Friedländer Reaction: Rapid, Scalable Mechanochemical Synthesis of Polyaryl‐substituted Quinolines

Advanced Synthesis & Catalysis, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 16, 2024

Abstract Quinolines are ubiquitous heterocyclic aromatic scaffolds, which can be found in many natural and synthetic products. They highly valued for their pharmacological electrochemical properties, encouraging the discovery of new routes quinoline synthesis diversification. In particular, polyaryl‐substituted quinolines have recently surged as useful substrates a wide variety applications, yet remain difficult inefficient. Herein, we report rapid novel mechanochemical Friedländer under basic conditions using ball milling. The optimized reaction resulted moderate to excellent yields ranging from 48% >95% demonstrated broad functional group tolerance at 1 hour times. We further show route photocatalyst DPQN 2,4−di−OMe photo‐ligand PPQN well OLED donor‐acceptor pCzPPQ, electron transport material oligoquinoline TQB organic semiconductor DPA. A gram scale was also achieved Resonant Acoustic Mixing (RAM), providing an isolated yield 87% after simple recrystallization ethanol.

Language: Английский

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Recent Advancements and Emerging Techniques in Nucleic Acid Isolation, Amplification, and Detection from Diverse Complex Matrices of Human Interest

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118172 - 118172

Published: Jan. 1, 2025

Language: Английский

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Fluorescent aminoquinolines responsive to micelles’ surface charge

Journal of Molecular Liquids, Journal Year: 2025, Volume and Issue: unknown, P. 127309 - 127309

Published: March 1, 2025

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Comprehensive Study of Thiazole‐Orange‐based DNA Dyes

ChemPhotoChem, Journal Year: 2024, Volume and Issue: unknown

Published: May 8, 2024

Abstract The rapid advancement of biotechnology over the recent decades has amplified importance DNA detection and quantification assays. Many these assays, such as gel electrophoresis, microscopy, flow cytometry, amplification in quantitative polymerase chain reaction (qPCR), rely on use DNA‐binding fluorescent dyes. This article presents a comprehensive study six Thiazole‐Orange‐based dyes: SYBR Safe, Green, Pico SYTO‐16, SYTO‐9, benzothiazole‐based analogue (TOPhBu) latter. selected markers were synthesized at 10‐milligram scale characterised spectroscopically to quantify their fluorescence enhancement upon binding double‐stranded DNA. ability dyes detect low concentrations was evaluated using two new metrics, absolute (AFE) relative (RFE). Quantum chemical calculations shed light mechanism fluorogenicity through modelling excited state behaviour Their analytical performance further tested qPCR experiments. experimental results this work highlight some important differences sensitivity efficiency studied which will facilitate marker selection for purposes future development novel sensors.

Language: Английский

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Elucidating the Interaction of Indole-3-Propionic Acid and Calf Thymus DNA: Multispectroscopic and Computational Modeling Approaches

Foods, Journal Year: 2024, Volume and Issue: 13(12), P. 1878 - 1878

Published: June 14, 2024

Indole-3-propionic acid (IPA) is a plant growth regulator with good specificity and long action. IPA may be harmful to human health because of its accumulation in vegetables fruits. Therefore, this study, the properties interaction between calf thymus DNA (ctDNA) were systematically explored using multispectroscopic computational modeling approaches. Analysis fluorescence spectra showed that binding ctDNA spontaneously form complex was mainly driven by hydrogen bonds hydrophobic interaction. melting analysis, viscosity cleavage circular dichroism measurement revealed groove did not significantly change confirmation. Furthermore, molecular docking found attached A-T rich minor region DNA. Molecular dynamics simulation formed stable caused slight fluctuations for residues at site. Gel electrophoresis experiments disrupt structure. These findings provide useful information on potential toxicological effects environmental risk assessments residue food level.

Language: Английский

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Investigation of the ability of 3-((4-chloro-6-methyl pyrimidin-2-yl)amino) isobenzofuran-1(3H)-one to bind to double-stranded deoxyribonucleic acid

Photochemical & Photobiological Sciences, Journal Year: 2024, Volume and Issue: 23(11), P. 2107 - 2121

Published: Nov. 1, 2024

Language: Английский

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Fluorescence-Based Simple and Practical Assay Method for DNA Damage Analysis in DNA-Encoded Library Synthesis

Bioconjugate Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

The dsDNA-selective fluorescent-dye-based DNA damage assay was developed for DNA-encoded library (DEL) synthesis. For the various DEL synthesis conditions, validated through cross-checking with high-performance liquid chromatography (HPLC) analysis, and fact confirmed that usage of a specific ratio organic solvent can critically induce damage. Also, applicability screening DNA-damaging condition on-DNA amide coupling reaction Pd-catalyzed N-arylation reaction.

Language: Английский

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