Vacuolar Sugar Transporter TMT2 Plays Crucial Roles in Germination and Seedling Development in Arabidopsis DOI Open Access
Yanting Cao, Jinju Hu, J. Q. Hou

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15852 - 15852

Published: Nov. 1, 2023

Vacuolar sugar transporters transport across the tonoplast, are major players in maintaining homeostasis, and therefore play vital roles plant growth, development, biomass yield. In this study, we analyzed physiological of tonoplast monosaccharide transporter 2 (TMT2) Arabidopsis. contrast to wild type (WT) that produced uniform seedlings, tmt2 mutant three types offspring: un-germinated seeds (UnG), seedlings cannot form true leaves (tmt2-S), develop normally (tmt2-L). Sucrose, glucose, fructose can substantially, but not completely, rescue abnormal phenotypes mutant. Abnormal cotyledon arrested leaf development shoot apical meristem (SAM) were observed tmt2-S seedlings. Cotyledons from WT tmt2-L restored growth through micrografting. Moreover, exogenous sustained normal with removed. Finally, found TMT2 deficiency resulted defects, most likely via changing auxin signaling, target rapamycin (TOR) pathways, cellular nutrients. This study unveiled essential functions for seed germination initial seedling ensuring function mobilizing sugars cotyledons It also expanded current knowledge on metabolism signaling. These findings have fundamental implications enhancing production or yield future agriculture.

Language: Английский

Effects of Shade Stress on the Synthesis of Cellulose and Lignin in Maize Nodal Roots DOI Open Access
Shengqin Liu,

Yuze Liu,

Shengqun Liu

et al.

Journal of Agronomy and Crop Science, Journal Year: 2025, Volume and Issue: 211(1)

Published: Jan. 1, 2025

ABSTRACT Low solar radiation is an important factor affecting maize root growth and development. Roots have anchoring function, their components are cellulose lignin. Here, shade experiments were conducted using nets with 50% light transmittance (L 50 ). The experiment was in 2021 2022 the ‘Xianyu 335’ variety under two nitrogen conditions (N 1 = 180 kg ha −1 N 2 240 ) to investigate effect of shading on structural carbohydrate content nodal roots. results showed that had a highly significant lignin contents. Compared normal 100 ), significantly decreased by 10.36%–13.87% 12.96%–18.68% Shading sucrose soluble sugar contents lignin‐related enzyme activities. positively correlated content. at silking (R1) stage synthase (SS) phosphate activities 15th leaf (V15) stage; R1 SS, acid invertase, tyrosine ammonia‐lyase, cinnamyl alcohol dehydrogenase, polyphenol oxidase peroxidase V15. Genes related synthesis, including ( SS CESA synthase‐interactive protein CSI1 Chitinase‐Like1 CTL1 STELLO2 STL2 downregulated shading, as synthesis‐related phenylalanine/tyrosine ammonia‐lyase PTAL 4‐coumarate‐CoA ligase 4CL POD genes. Auxin jasmonic affected thereby reducing synthesis. These findings provide theoretical support for development appropriate cultivation practices reduced radiation.

Language: Английский

Citations

1
Telomere-to-telomere Phragmites australis reference genome assembly with a B chromosome provides insights into its evolution and polysaccharide biosynthesis DOI Creative Commons
Jipeng Cui, Rui Wang,

Ruoqing Gu

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 17, 2025

Phragmites australis is a globally distributed grass species (Poaceae) recognized for its vast biomass and exceptional environmental adaptability, making it an ideal model studying wetland ecosystems plant stress resilience. However, genomic resources this have been limited. In study, we assembled chromosome-level reference genome of P. containing one B chromosome. An explosion LTR-RTs, centered on the Copia family, occurred during late Pleistocene, driving expansion size subgenomic differentiation. Comparative analysis showed that underwent two whole gene duplication events, was segregated from Cleistogenes songorica at 34.6 Mya, 41.26% families expansion. Based multi-tissue transcriptomic data, identified structural genes in biosynthetic pathway pharmacologically active Phragmitis rhizoma polysaccharides with essential roles rhizome development. This study deepens our understanding Arundinoideae evolution, dynamics, genetic basis key traits, providing data foundation restoration, bioenergy development, stress.

Language: Английский

Citations

1
Effects of retaining different number of mother bamboo on the growth and NSCs allocation of new-born seedlings in Phyllostachys edulis at different age DOI Creative Commons
Binao Zhou,

Xu Zhang,

Hanjiao Zhang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 16, 2025

Non-structural carbohydrates (NSCs) are key substances for metabolic processes in plants, providing energy growth, development, and responses to environmental stress. Pruning mother bamboo a clump can significantly affect the NSCs allocation of new shoots, thereby affecting their growth. Moso (Phyllostachys edulis) is an important economic species with highest planting area China. However, it remains unclear how many left maximize influence moso seedlings at different age. This study investigated effects retaining number on morphological characterization new-born two-year-old three-year-old, respectively. Retaining more promoted plant height diameter particularly clumps. The growth rate shoots increased clumps, while remained relatively stable three-year-old strategy also showed significant differences when across stages. content continuously during rapid phase, which reached peak all parts end rhizome elongation stage. In seedlings, retained plants. Significant were found between treatments, those one four bamboos left. It was similar there no among treatments. Reducing appropriately promote bamboo. clumps dependent bamboo, less pronounced production practice, recommended that three two approach expand afforestation ensuring survival

Language: Английский

Citations

0
Transcriptome profiling reveals the effect of disbudding on growth of young tea plants (Camellia sinensis) DOI Creative Commons
Dandan Tang, Lu Long,

Lufang Feng

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120691 - 120691

Published: Feb. 17, 2025

Language: Английский

Citations

0
The Physiological and Ecological Processes of Anthocyanins Accumulation and Red Gall Coloration DOI
Dênis Coelho de Oliveira, Moshe Inbar, Simcha Lev‐Yadun

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Carbohydrate distribution via SWEET17 is critical for Arabidopsis inflorescence branching under drought DOI
Marzieh Valifard, Azkia Khan,

Johannes Berg

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(13), P. 3903 - 3919

Published: March 26, 2024

Abstract Sugars Will Eventually be Exported Transporters (SWEETs) are the most recently discovered family of plant sugar transporters. By acting as uniporters, SWEETs facilitate diffusion sugars across cell membranes and play an important role in various physiological processes such abiotic stress adaptation. AtSWEET17, a vacuolar fructose facilitator, was shown to involved modulation root system during drought. In addition, previous studies have that overexpression apple homolog leads increased drought tolerance tomato plants. Therefore, SWEET17 might molecular element responses However, function above-ground tissues Arabidopsis under remain elusive. combining gene expression analysis stem architecture with profiles different tissues, we uncovered putative for carbohydrate supply thus cauline branch elongation, especially periods carbon limitation, occurs stress. Thus, seems maintaining efficient reproduction conditions.

Language: Английский

Citations

3
Senescence-Associated Sugar Transporter1 affects developmental master regulators and controls senescence in Arabidopsis DOI
Jintao Cheng, Meerim Arystanbek Kyzy, A. Heide

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 196(4), P. 2749 - 2767

Published: Aug. 19, 2024

Abstract Sugar transport across membranes is critical for plant development and yield. However, an analysis of the role intracellular sugar transporters in senescence lacking. Here, we characterized Senescence-Associated Transporter1 (SAST1) during Arabidopsis (Arabidopsis thaliana). SAST1 expression was induced leaves after application abscisic acid (ABA). a vacuolar protein that pumps glucose out cytosol. sast1 mutants exhibited stay-green phenotype developmental senescence, darkening single leaves, ABA feeding. To explain mutants, analyzed activity glucose-induced master regulator TOR (target rapamycin), which responsible maintaining high anabolic state. higher compared to wild types, whereas its antagonist, SNF1-related kinase 1 (SnRK1), reduced under senescent conditions. This deregulation SnRK1 activities correlated with cytosolic levels conditions mutants. Although displayed functional phenotype, their seed yield reduced. These analyses place last phase leaf's existence molecular program required complete life cycle.

Language: Английский

Citations

3
Transcriptome and Metabolome Analyses Offer New Insights into Bolting Time Regulation in Broccoli DOI Open Access

Linqian Kuang,

Yue Zhang, Nan Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3726 - 3726

Published: April 15, 2025

The globular buds and stems are the main edible organs of broccoli. Bolting is an important agronomic trait, timing its occurrence particularly critical when breeding domesticating molecular mechanism that regulates broccoli bolting time not well-understood. In this study, apical flower bud leaf tissues two varieties with different intensities were selected for metabolome transcriptome analyses. early-bolting B2554 late-bolting B2557, 1094 differentially expressed genes 206 accumulated metabolites identified. leaves, 487 40 floral pathway, expression FLC (FLOWERING LOCUS C) was significantly upregulated, FT T) downregulated in plants, indicating their possible role suppressing bolting. addition, significant differences identified sucrose synthesis transport, hormone synthesis, signal transduction processes B2557. Sucrose accumulation leaves plants about 1.3 times higher than plants. Indole-3-acetic acid (IAA) abscisic (ABA) more twice Jasmonic (JA) ten Phenolic acids may affect This study offers new insights into regulation provides some potential targets to include methods regulate time.

Language: Английский

Citations

0
Widely-targeted metabolomics and transcriptomics identify metabolites associated with flowering regulation of Choy Sum DOI Creative Commons
Xinmin Huang, Yunna Zhu, Wei Su

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 9, 2024

Abstract Choy Sum, a stalk vegetable highly valued in East and Southeast Asia, is characterized by its rich flavor nutritional profile. Metabolite accumulation key factor Sum development; however, no research has focused on metabolic changes during the development of especially shoot tip metabolites, their effects growth flowering. Therefore, present study, we used widely targeted metabolomic approach to analyze metabolites stalks at seedling (S1), bolting (S3), flowering (S5) stages. In total, identified 493 31 chemical categories across all three developmental We found that levels most carbohydrates amino acids increased peaked S5. Moreover, was closely related G6P, whereas expression genes content T6P, which may promote upregulating expressions BcSOC1 , BcAP1 BcSPL5 . The results this study contribute our understanding relationship between stem substances regulatory mechanisms other species.

Language: Английский

Citations

2
Combination of transcriptomic, biochemical, and physiological analyses reveals sugar metabolism in Camellia drupifera fruit at different developmental stages DOI Creative Commons
Zhen Liu,

C.S. SHEN,

Ruifan Chen

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 13, 2024

Camellia drupifera , a significant woody oil crop in southern China, produces from its fruit seeds. Understanding sugar metabolism enzyme regulation is crucial for accumulation and synthesis organs. This study examines the dynamic changes across four developmental stages of C. fruits, rapid enlargement to conversion. We analyzed content, activity, transcriptomic data identify key periods mechanisms involved metabolism. Our findings indicate that photosynthetic products are rapidly transported leaves organs after synthesis, with transport efficiency decreasing significantly 48 hours. September was identified as critical period conversion, during which highest sucrose levels SuSy-II activity were detected kernels. A positive correlation found between high expression ten genes related enzymes proteins content. Notably, c158337.graph_c0 (SPS), c166323.graph_c0 (SuSy), c159295.graph_c0 (SUC2-like), c156402.graph_c0 (SUC2-like) increased conversion phase.These provide theoretical foundation elucidating molecular offering insights could enhance economic yield.

Language: Английский

Citations

2