Vacuolar Sugar Transporter TMT2 Plays Crucial Roles in Germination and Seedling Development in Arabidopsis DOI Open Access
Yanting Cao, Jinju Hu, J. Q. Hou

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15852 - 15852

Published: Nov. 1, 2023

Vacuolar sugar transporters transport across the tonoplast, are major players in maintaining homeostasis, and therefore play vital roles plant growth, development, biomass yield. In this study, we analyzed physiological of tonoplast monosaccharide transporter 2 (TMT2) Arabidopsis. contrast to wild type (WT) that produced uniform seedlings, tmt2 mutant three types offspring: un-germinated seeds (UnG), seedlings cannot form true leaves (tmt2-S), develop normally (tmt2-L). Sucrose, glucose, fructose can substantially, but not completely, rescue abnormal phenotypes mutant. Abnormal cotyledon arrested leaf development shoot apical meristem (SAM) were observed tmt2-S seedlings. Cotyledons from WT tmt2-L restored growth through micrografting. Moreover, exogenous sustained normal with removed. Finally, found TMT2 deficiency resulted defects, most likely via changing auxin signaling, target rapamycin (TOR) pathways, cellular nutrients. This study unveiled essential functions for seed germination initial seedling ensuring function mobilizing sugars cotyledons It also expanded current knowledge on metabolism signaling. These findings have fundamental implications enhancing production or yield future agriculture.

Language: Английский

Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems DOI Creative Commons
Neda Fakhimi, Arthur Grossman

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 3015 - 3015

Published: Oct. 28, 2024

Photosynthetic eukaryotes have metabolic pathways that occur in distinct subcellular compartments. However, because metabolites synthesized one compartment, including fixed carbon compounds and reductant generated by photosynthetic electron flows, may be integral to processes other compartments, the cells must efficiently move among different This review examines various flows used generate ATP trafficking of green alga Chlamydomomas reinhardtii; information on algae plants is provided add depth nuance discussion. We emphasized across envelope membranes two energy powerhouse organelles cell, chloroplast mitochondrion, nature roles major mobile these specific or presumed transporters involved trafficking. These include sugar-phosphate (sugar-P)/inorganic phosphate (Pi) dicarboxylate transporters, although, many cases, we know little about substrate specificities how their activities are regulated/coordinated, compensatory responses when compromised, associations between cellular proteins, possibilities for forming ‘megacomplexes’ involving interactions enzymes central metabolism with transport proteins. Finally, discuss metabolite associated biological under environmental conditions help maintain cell’s fitness. C4 concentrating mechanism, photorespiration, fermentation algae.

Language: Английский

Citations

2

Glucose enhanced lignin accumulation in grapevine stems via promoting phenylpropanoid biosynthesis DOI Creative Commons
Han Wang, Juanjuan Huang, Congcong Zhang

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 8, 2024

The lignification of branches can promote the accumulation nutrients, increase plant survival and resistance to biotic abiotic stresses. As an important carbon source for plants, glucose is also skeleton lignin synthesis. Grapevine a perennial cash crop, highly lignified are essential ensure growth grapevine development fruit. Here, 'Red Globe' grape (Vitis vinifera L.) plantlets were selected as material cultured with different concentrations glucose: 0 g/L, 20 40 g/L 60 (G0, G20, G40 G60). Among them, G0 group control. Lignin, anthocyanins contents, height microstructure measured at each concentration after 40-, 50-, 60-day treatments. Transcriptome metabolome used analyze difference in genes metabolites 50 days growth. After cultivation, content was highest. And xylem cells number increased. To further, transcriptome have identified total 3638 differentially expressed (DEGs) (including 245 TFs) 510 differently accumulated (DAMs) three comparison groups. In-depth joint analysis revealed that phenylpropanoid biosynthesis pathway significantly respond exogenous glucose, 37 DEGs identified. Therefore, phenylpropane may be key levels grapes, differential expression being top priority. These findings provide new perspective understanding relationship between grape.

Language: Английский

Citations

1

Functional crosstalk of sucrose and G protein signaling in maize thermotolerance by modulating osmoregulation system DOI

Hong-Yan Chen,

Zhong‐Guang Li

PROTOPLASMA, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Language: Английский

Citations

1

Telomere-to-telomere Phragmites australis reference genome assembly with a B chromosome provides new insights into its evolution and polysaccharide biosynthesis DOI Creative Commons

Suxia Cui,

Jipeng Cui, Rui Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 18, 2024

Abstract Phragmites australis , widely distributed worldwide, has a vast biomass and strong ability to adapt the environment. Its rhizome (known as "Lugen" in Chinese medicine) been used traditional medicine for over 2,000 years. In this study, we assembled chromosome-level reference genome of containing one B chromosome. An explosion LTR-RTs, centered on Copia family, occurred during late Pleistocene, driving expansion reeds size subgenomic differentiation. Comparative genomic analysis showed that P. underwent two whole gene duplication events, was segregated from Cleistogenes songorica at 34.6 Mya, 41.26% families expansion. Based multi-tissue transcriptomic data, identified structural genes biosynthetic pathway pharmacologically active polysaccharides have important roles development. The assembly contributes our understanding Arundiaceae evolution polysaccharide biosynthesis phragmitis rhizoma .

Language: Английский

Citations

1

Metabolism and development DOI
Alisdair R. Fernie, Uwe Sonnewald, Arun Sampathkumar

et al.

Journal of Plant Physiology, Journal Year: 2024, Volume and Issue: 295, P. 154208 - 154208

Published: Feb. 28, 2024

Language: Английский

Citations

0

Role of BraSWEET12 in Regulating Flowering through Sucrose Transport in Flowering Chinese Cabbage DOI Creative Commons

Qinqin He,

Li-Ming He,

Zongqin Feng

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(10), P. 1037 - 1037

Published: Sept. 29, 2024

We assessed the flowering Chinese cabbage (Brassica rapa var. parachinensis), a specialty vegetable found in southern China. The sugar content of stem tip is closely related to bolting and flowering. Sugar Will Eventually be Exported Transporters (SWEETs) are bidirectional transporter proteins involved numerous plant growth development processes. expression BraSWEET12 positively correlated with content. However, it unclear whether In this study, we identified characterized BraSWEET12. contains 288 amino acids located on cell membrane as sucrose protein. highly expressed petals tips upregulated by gibberellin low temperatures. Overexpression Arabidopsis can increase at tip, upregulate AtAP1 AtLFY, advance time. Subsequently, our results indicate that accumulation plays crucial role regulation. These provide reference for elucidating regulatory mechanisms underlying

Language: Английский

Citations

0

Dynamic changes of endogenous phytohormones and carbohydrates during spontaneous morphogenesis of Centaurium erythraea Rafn DOI Creative Commons
Milana Trifunović‐Momčilov, Václav Motyka,

Marija Marković

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 6, 2024

Common centaury ( Centaurium eryhtraea Rafn) is a medicinal plant species with vigorous morphogenic potential in vitro . The process of spontaneous shoot regeneration solid root culture characteristic for this species. In context, the aim work was to investigate dynamic changes endogenous phytohormones and carbohydrates content explants at different time points (0, 2, 4, 7, 14, 21, 28, 60 days) during morphogenesis Detailed analysis cytokinins (CKs) showed that trans -zeatin tZ ) major bioactive CK all points. corresponding riboside, t Z9R, also determined majority identified transport forms, time-points. Further auxin revealed significant increase indole-3-acetic acid (IAA) after 21 days, when huge jump ratio IAA/bioactive CKs observed. maximum total soluble sugar measured 14 while decrease first regenerated adventitious shoots appeared. This undoubtedly indicates an increased energy requirement prior actual shoots. obtained results indicate period from day involves most dramatic disturbances CKs, IAA carbohydrate balance, which are very important valuable factors onset regeneration.

Language: Английский

Citations

0

Vacuolar Sugar Transporter TMT2 Plays Crucial Roles in Germination and Seedling Development in Arabidopsis DOI Open Access
Yanting Cao, Jinju Hu, J. Q. Hou

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15852 - 15852

Published: Nov. 1, 2023

Vacuolar sugar transporters transport across the tonoplast, are major players in maintaining homeostasis, and therefore play vital roles plant growth, development, biomass yield. In this study, we analyzed physiological of tonoplast monosaccharide transporter 2 (TMT2) Arabidopsis. contrast to wild type (WT) that produced uniform seedlings, tmt2 mutant three types offspring: un-germinated seeds (UnG), seedlings cannot form true leaves (tmt2-S), develop normally (tmt2-L). Sucrose, glucose, fructose can substantially, but not completely, rescue abnormal phenotypes mutant. Abnormal cotyledon arrested leaf development shoot apical meristem (SAM) were observed tmt2-S seedlings. Cotyledons from WT tmt2-L restored growth through micrografting. Moreover, exogenous sustained normal with removed. Finally, found TMT2 deficiency resulted defects, most likely via changing auxin signaling, target rapamycin (TOR) pathways, cellular nutrients. This study unveiled essential functions for seed germination initial seedling ensuring function mobilizing sugars cotyledons It also expanded current knowledge on metabolism signaling. These findings have fundamental implications enhancing production or yield future agriculture.

Language: Английский

Citations

0