Changes in Characteristics of Spermatogonial Stem Cells in Response to Heat Stress in Stallions

Theriogenology, Journal Year: 2024, Volume and Issue: 224, P. 74 - 81

Published: May 7, 2024

Language: Английский

Quantitative Proteomic Analysis Reveals Key Proteins Involved in Testicular Development of Yaks

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8433 - 8433

Published: Aug. 2, 2024

Male reproductive health is largely determined already in the early development of testis. Although much work has been carried out to study mechanisms testicular and spermatogenesis, there was previously no information on differences protein composition yak testicles during development. In this study, profiles 6- (M6), 18- (M18), 30-month-old (M30) yaks were comparatively analyzed using TMT proteomics. A total 5521 proteins identified, with 13, 1295, 1397 differentially expressed (DEPs) 30- vs. 18-, 6-, 6-month-old testes, respectively. Gene Ontology (GO) annotation Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis showed that DEPs mainly involved signaling pathways related including MAPK, PI3K–Akt, Wnt, mTOR, TGF-β, AMPK pathways. Furthermore, we also identified eight potential (TEX101, PDCL2, SYCP2, SYCP3, COL1A1, COL1A2, ADAM10, ATF1) may be spermatogenesis yaks. This provide new insights into molecular

Language: Английский

Integrated Quantitative Proteomics and Phosphoproteomics Analysis Reveals the Dynamic Process of Buffalo (Bubalus bubalis) Spermatogenesis

Reproduction in Domestic Animals, Journal Year: 2024, Volume and Issue: 59(12)

Published: Dec. 1, 2024

ABSTRACT Spermatogenesis is a highly complex and tightly regulated cellular differentiation process closely related to the productive performance of male livestock. We do not yet have clear understanding spermatogenesis mechanism buffalo. In this study, spermatogonia, spermatocytes spermatids were analysed by flow cytometry. Quantitative proteomic phosphoproteomic studies performed on different spermatogenic cells using tandem mass tagging technology liquid chromatography–tandem spectrometry. A total 219 differentially expressed proteins (involved in focal adhesions actin cytoskeleton pathways) 71 phosphoproteins RNA transport adhesion junction obtained. Through trend analysis, dynamic profile protein expression was obtained, enriched main biological processes at stages spermatogenesis. By immunohistochemical localisation it found that MACROH2A2, TOP2A, LMNA, LMNA (pS392), VIM (pS56) had specific testis cells. Network analysis kinase‐substrate phosphorylation sites showed AKT1 most active kinase, kinases can catalyse LMNA. This study provides reference for studying molecular buffalo helps clarify regulatory translation post‐translational modification during mammalian

Language: Английский