Detection of In-Vitro Antibacterial Activity of Tinospora cordifolia Leaf Extracts against Multidrug Resistant- Staphylococcus aureus Isolated from Diabetic Foot Infections DOI

Sehrish Mahmood,

Sher Bahadar Khan, Akhtar Ali

et al.

Pakistan Journal of Health Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 226 - 231

Published: Sept. 30, 2024

The manifestations of diabetes are always a great challenge to the medical practitioners and one its challenging ones Diabetic Foot Infection (DFI). There multiple ways managing this condition such as using antibiotics eliminate bacteria boosting patient’s intrinsic factors, for example, through blood glucose optimization. DFIs difficult treat nowadays because antibiotic resistance; result, scientists have turned medicinal plants finding drugs against Multi-Drug Resistant (MDR) bacterial strains. Tinospora cordifolia is promising plant with untapped wealth chemical compounds high therapeutic potential. These biologically active metabolites work together different mechanisms causing antibacterial action MDR Objective: To assess potential Leaf Extract (TcLE) MDR-Staphylococcus aureus isolated from pus samples patients DFIs. Methods: In-vitro experimental study conducted in Ziauddin University December 2022 September 2023. Extraction TcLE was done rotary evaporator. activity evaluated by Agar well diffusion assay. Results: Eight doses were prepared 10% DMSO TC's ethanolic leaf extracts. Growth strain inhibited at tested concentration 250 mg/ml, which MIC (the lowest suppressed growth strains). Conclusion: showed aureus, thus establishing it lead compound source anti-staphylococci drugs.

Language: Английский

Antibacterial, Antibiofilm, and Wound Healing Activities of Rutin and Quercetin and Their Interaction with Gentamicin on Excision Wounds in Diabetic Mice DOI Creative Commons
Yasir Almuhanna, Abdulrahman Alshalani, Hamood AlSudais

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 676 - 676

Published: Aug. 29, 2024

Phytochemicals are effective and gaining attention in fighting against drug-resistant bacterial strains. In the present study, rutin quercetin were tested for antibacterial, antibiofilm, wound healing activities on excision wounds infected with MDR-P. aeruginosa diabetic mice. Antibacterial antibiofilm studied vitro using broth dilution assay crystal violet assay, respectively. These phytochemicals alone wound-healing at different concentrations (0.5% 1% ointment base) combination gentamicin to evaluate any additive effects. Rutin demonstrated effectiveness higher concentrations. Both inhibited biofilm formation contributed of by eradicating wounded tissue. a low concentration (0.5%) had lesser effect reducing epithelization period regeneration epithelial layer compared quercetin. When combined gentamicin, (1%) displayed maximum epithelium regeneration, followed gentamicin. found be more controlling when used as an The study supports traditional use managing infections.

Language: Английский

Citations

5
Interpenetrating Polymer Networks in Biomedical Fields: Recent Advanced and Applications DOI Open Access

Farangis Shahi,

Sara Zarei,

Razhan Salah Othman

et al.

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(2)

Published: Feb. 1, 2025

ABSTRACT Recent developments in polymer materials have led to an increased implementation of hydrogels biomedical settings, especially the creation smart hydrogels. Traditional single‐network often exhibit challenges, such as poor mechanical strength, insufficient biocompatibility, and slow response rates. To address these issues, researchers introduced Interpenetrating Polymer Network (IPN) hydrogels, which significantly improve strength via topological entanglements physical interactions. This dual‐network design not only enhances biocompatibility but also responsiveness stimuli, endowing with distinctive properties like cell adhesion, conductivity, hemostatic functions, antioxidant abilities, color‐changing properties. The purpose this article is elucidate factors that trigger stimuli IPN their impacts on cellular behavior, various applications they can serve. A comprehensive overview provided regarding classification, mechanisms, performance attributes, related subjects. Ultimately, review emphasizes promise hold fulfilling increasing need for innovative improved features sector.

Language: Английский

Citations

0
Molecular characterization of resistance and biofilm genes of ESKAPE pathogens isolated from clinical samples: examination of the effect of boric acid on biofilm ability by cell culture method DOI Creative Commons
Özgür Çelebi, Sümeyye BAŞER, Mustafa Can Güler

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 1, 2025

Biofilm formation ranks first among the resistance and virulence factors crucial in forming ESKAPE pathogens. Once biofilm is formed, treating infection with existing drugs often futile. Therefore, this study, resistant pathogens were isolated from intensive care units sent to Atatürk University Yakutiye Research Hospital Microbiology Laboratory. This study investigated molecular characterization of units. The bacteria's abilities, genes responsible for formation, characteristics identified. effect boric acid (BA) on bacterial was evaluated by a cell culture model. highest observed Escherichia coli, Enterococcus spp., Pseudomonas aeruginosa spp. isolates showed vanA gene 14.6% vanC 61% samples. Among Staphylococcus isolates, 48.3% MSSA, 34.5% MRCNS, 17.2% MRSA. KPC detected 50%, OXA-48 40%, NDM 15% isolates. In P. aeruginosa, LasI LasR quorum sensing system found 38.5% 30.8% respectively. E. coli present 35%, 31.7%, TEM 12.5%. BA demonstrated significant activity against combined antimicrobial boron compounds decrease expression level gene. It will be promising preventing hospital-associated infections.

Language: Английский

Citations

0
Multidrug resistance, biofilm formation, and genetic determinants in diabetic foot infections from Uttar Pradesh, India: a clinical-microbiological insight from a prospective study DOI
Mohd Saleem, Mohd Shahid Khan,

Arslan Neyaz

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

Language: Английский

Citations

0
Advanced Drug Delivery Systems for Topical Insulin in Diabetic Wound Healing DOI

Sheida Barkhordari,

Farangis Shahi,

Hossein Ali Khonakdar

et al.

ChemistrySelect, Journal Year: 2024, Volume and Issue: 9(44)

Published: Nov. 1, 2024

Abstract Diabetes mellitus is a chronic condition characterized by impaired insulin production or utilization, leading to serious complications such as diabetic foot ulcers (DFUs). DFUs are wounds with high levels of inflammatory cytokines, arterial occlusion, and persistent infection, often resulting in significant morbidity. Traditional treatment methods, including debridement, offloading, infection control, have shown limited success, prompting the exploration novel therapeutic strategies. Insulin, known for its role glucose metabolism, also possesses wound‐healing properties promoting cell proliferation, protein synthesis, modulating inflammation. However, systemic administration not ideal due hypoglycemia risks difficulties achieving concentrations at wound sites. This review focuses on advanced drug delivery systems topical application dressings. Various platforms, hydrogels, nanoparticles, liposomes, microemulsions been developed optimize bioavailability, protect it from degradation, ensure controlled release. These aim enhance management addressing multifactorial nature healing patients. The provides comprehensive overview current advancements, evaluates efficacy these preclinical clinical studies, discusses challenges future perspectives this field. By highlighting potential innovative approaches, underscores promise improving patient outcomes.

Language: Английский

Citations

2
Prevalence of carbapenem-resistant Enterobacterales (CRE) in Saudi Arabia: A systematic review and meta-analysis DOI Creative Commons
Ahmad A. Alshehri, Ahmad Adebayo Irekeola

Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(11), P. 102186 - 102186

Published: Oct. 14, 2024

Language: Английский

Citations

1
Detection of In-Vitro Antibacterial Activity of Tinospora cordifolia Leaf Extracts against Multidrug Resistant- Staphylococcus aureus Isolated from Diabetic Foot Infections DOI

Sehrish Mahmood,

Sher Bahadar Khan, Akhtar Ali

et al.

Pakistan Journal of Health Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 226 - 231

Published: Sept. 30, 2024

The manifestations of diabetes are always a great challenge to the medical practitioners and one its challenging ones Diabetic Foot Infection (DFI). There multiple ways managing this condition such as using antibiotics eliminate bacteria boosting patient’s intrinsic factors, for example, through blood glucose optimization. DFIs difficult treat nowadays because antibiotic resistance; result, scientists have turned medicinal plants finding drugs against Multi-Drug Resistant (MDR) bacterial strains. Tinospora cordifolia is promising plant with untapped wealth chemical compounds high therapeutic potential. These biologically active metabolites work together different mechanisms causing antibacterial action MDR Objective: To assess potential Leaf Extract (TcLE) MDR-Staphylococcus aureus isolated from pus samples patients DFIs. Methods: In-vitro experimental study conducted in Ziauddin University December 2022 September 2023. Extraction TcLE was done rotary evaporator. activity evaluated by Agar well diffusion assay. Results: Eight doses were prepared 10% DMSO TC's ethanolic leaf extracts. Growth strain inhibited at tested concentration 250 mg/ml, which MIC (the lowest suppressed growth strains). Conclusion: showed aureus, thus establishing it lead compound source anti-staphylococci drugs.

Language: Английский

Citations

0