Serum Starvation Enhances the Antitumor Activity of Natural Matrices: Insights into Bioactive Molecules from Dromedary Urine Extracts

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 821 - 821

Published: Feb. 10, 2025

Natural matrices have historically been a cornerstone in drug discovery, offering rich source of structurally diverse and biologically active compounds. However, research on natural products often faces significant challenges due to the complexity matrices, such as urine, limitations bioactivity assessment assays. To ensure reliable insights, it is crucial optimize experimental conditions reveal bioactive potential samples, thereby improving validity statistical analyses. Approaches metabolomics further strengthen this process by identifying focusing most promising compounds within enhancing precision metabolite prioritization. In study, we assessed 17 dromedary urine samples human renal cells under serum-reduced (1%FBS) order minimize possible FBS-derived interfering factors. Using viability assays Annexin V/PI staining, found that tumor cell lines Caki-1 RCC-Shaw were more sensitive cytotoxic effects small molecules present compared non-tumor HK-2 cells. Employing NMR analysis combined with detected vitro activity, our model highlights presence azelaic acid phenylacetyl glycine, underscoring its sustainable framework green chemistry circular economy initiatives.

Language: Английский

Exosomes derived from umbilical cord blood NK cells inhibit the progression of pancreatic cancer by targeting ROS-mediated mitochondrial dysfunction

Saudi Pharmaceutical Journal, Journal Year: 2025, Volume and Issue: 33(1-2)

Published: April 29, 2025

Language: Английский