Novel Tear Biomarkers in Ocular Graft Versus Host Disease Associated with Th1/Th2 Immune Responses: A Case Series and Literature Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4311 - 4311

Published: May 1, 2025

Ocular graft versus host disease (oGVHD) is a common complication of allogeneic hematopoietic stem cell transplantation and may be associated with dry eye chronic inflammation fibrosis. Immune dysregulation, particularly the Th1/Th2 imbalance, plays key role in progression oGVHD. This case study presents two oGVHD patients (a 20-year-old acute 59-year-old oGVHD), analyzing clinical parameters (Schirmer test I, tear film break-up time, Surface Disease Index (OSDI), kerato-conjunctival staining) alongside biomarkers. A 27-plex cytokine analysis was performed using Luminex200 platform, assessing various biomarkers against control group-defined normal range. Key included beta2-microglobulin (β2-MG), complement components, chemokines, growth factors, both pro-inflammatory anti-inflammatory cytokines, as well series soluble ligand receptors. The identified distinct biomarker patterns during topical corticosteroid treatment oGHVD patient, suggesting potential shifts responses progressed. Notably, CD27, TNF-related apoptosis-inducing (TRAIL) receptor 2 (TRAIL-R2), chemokine (CCL2), IL-1β, initially elevated, normalized treatment, while tear-soluble Fas remained highly elevated (>400-fold). Conversely, TRAIL, which at very low levels (100-fold lower), increased reached levels, coinciding improvements ocular symptoms OSDI score. also highlights differences between oGVHD, novel such TRAIL/TRAIL-R2, CCL2. Enhancing our understanding dynamics improve monitoring pave way for personalized management strategies to patient outcomes.

Language: Английский

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Corneal Sensitivity and Neuropathy in Patients With Ocular Graft-Versus-Host Disease

Cornea, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

To assess corneal sensitivity changes in patients with ocular graft-versus-host disease using a non-contact and Cochet-Bonnet esthesiometer. In addition, we evaluate the association between subbasal nerve epitheliopathy these patients.

Language: Английский

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Umbilical Cord Blood Platelet Lysate Eyedrops for the Treatment of Severe Ocular Surface Disorders in Graft vs. Host Disease Patients: Clinical Study

Life, Journal Year: 2024, Volume and Issue: 14(10), P. 1268 - 1268

Published: Oct. 5, 2024

Graft-versus-host disease (GvHD) is an overactive systemic inflammatory response that can arise following allogeneic hematopoietic stem cell transplantation (HSCT). This condition occurs when the transplanted donor immune cells recognize recipient's tissues as foreign and trigger against them. The ocular surface (eyelids, conjunctiva, meibomian glands, lacrimal cornea) particularly involved in GvHD, its to existing treatments, including potent immunosuppressants new targeted therapies, undesirable, with such treatments often being ineffective. Human umbilical cord blood platelet lysate stands out a adjunct conventional therapies for disorders related severe Dry Eye Disease. study aimed evaluate safety efficacy of eyedrops treatment graft-versus-host patients who have received previous unsuccessful treatments.

Language: Английский

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Concurrent Limbal Stem Cell Deficiency and Mild Neurotrophic Keratopathy in Graft-Vs-Host Disease

Cornea, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

The purpose of this study was to delineate the concurrence limbal stem cell deficiency (LSCD) and neurotrophic keratopathy in patients with ocular graft-vs-host disease (oGVHD).

Language: Английский

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