Urbanization Shifts Immunometabolism in a Common Bumblebee DOI Creative Commons
Virginie Cuvillier‐Hot, Alessandro Fisogni, Vincent Doublet

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(12)

Published: Dec. 1, 2024

ABSTRACT The growing urbanization process is accompanied by the emergence of new habitats for wildlife, and cities are sometimes seen as refuges pollinators such wild bees compared to intensively cultivated rural habitats. However, contrasting living conditions that combine high fragmentation, exposure pollutants, heat island effects, with low pesticide use potentially availability resources, make it difficult predict overall effect urban on health bees. Moreover, if responses bee populations in terms species richness diversity have been focus many recent studies, individual more rarely investigated. More specifically, data impacts physiology lacking. To help fill this gap, we collected red‐tailed bumblebee ( Bombus lapidarius ) workers along a gradient defined level soil imperviousness, estimated environmental (air quality) ecological (pathogens' prevalence loads; local competition) pressures they locally experienced. In parallel, quantified expression selected immune marker genes. We measured how system bumblebees responds which parameters best explain observed changes gene expression. evidenced three markers, tightly linked cellular metabolism, whose expressions increase urbanization, independently infection pollution exposure. suggest induction their reveals shift immunometabolism, supposedly response stressful experienced areas built‐up cover. these genes likely at root any activation; could thus be used markers estimate levels stress pollinators.

Language: Английский

Influence of Hyperthermia Treatment on Varroa Infestation, Viral Infections, and Honey Bee Health in Beehives DOI Creative Commons
Xinjian Xu, Shujing Zhou, Jian Huang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 168 - 168

Published: Feb. 5, 2025

The mite Varroa destructor is widely acknowledged as the most destructive threat to honey bee (Apis mellifera) colonies on a global scale. infestations in are intricately linked with viral infections, collaboratively leading diminished populations and accelerated colony losses. Extensive research has firmly established correlation between varroa mites viruses, underscoring mite’s efficiency spreading viruses among bees colonies. effective control of expected result decrease infections within Research suggests that thermal treatments (hyperthermia) present viable approach combat mites, studies demonstrating role heat stress reducing affected bees. This article examines extant literature surrounding utilization hyperthermia potential method ameliorate adverse impacts their associated It also outlines characteristics these stressors. Diverse devices can be used for subjecting treatment, targeting both outside brood cells. application treatments, typically ranging 40 42 °C 1.5–3 h, reduce shown promise. Notably, precise effectiveness treatment comparison alternative measures remains uncertain available literature. deleterious repercussions this mechanism immature mature evaluated. Concurrently, detrimental implications prolonged durations discussed. Regarding impact them negatively by either or inducing production shock proteins possess antiviral properties. Various factors identified influential efficacy colonies, including device type duration, necessitating further empirical investigations. Additionally, highlights existing gaps knowledge provides insights into prospective directions concerning method.

Language: Английский

Citations

1
Genome-Wide Identification and Characterization of Heat Shock Proteins in the Stored-Product Pest Rhyzopertha dominica (Fabricius): Phylogenetic, Structural, and Stress-Induced Expression Analyses DOI Creative Commons
Yueliang Bai, Yan Xie, Jiayu Yao

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 127 - 127

Published: Jan. 28, 2025

Heat shock proteins (HSPs) are crucial molecular chaperones that help organisms maintain protein stability under stress conditions. As a major stored-product pest, Rhyzopertha dominica (Fabricius) faces distinct stresses compared to field insects, primarily due the specific pest control methods applied during grain storage. In this study, total of 53 HSP genes from five gene families (HSP90, HSP70, HSP60, sHSP, and DnaJ) were identified characterized using bioinformatics methods. Among them, DnaJ was largest most diverse family in R. dominica. Transcriptome sequencing RT-qPCR then used evaluate expression patterns four storage-related stresses, following series bioassays. Extreme high temperature strongest inducer expression, with 12 showing over 10-fold increase. Controlled nitrogen atmosphere also led considerable upregulation genes, especially HSP70 family. contrast, phosphine fumigation K-Obiol protectant caused very limited induction which might have been less severe damage by chemical physical stresses. Our study provides theoretical basis for further research on functions

Language: Английский

Citations

0
Factors affecting heat resilience of drone honey bees (Apis mellifera) and their sperm DOI Creative Commons
Alison McAfee, Bradley N. Metz,

Patrick Connor

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0317672 - e0317672

Published: Feb. 7, 2025

Extreme temperatures associated with climate change are expected to impact the physiology and fertility of a variety insects, including honey bees. Most previous work on this topic has focused female bees (workers queens), comparatively little research investigated how heat exposure affects males (drones). To address gap, we tested body mass, viral infections, population origin as predictors drone survival sperm viability in series challenge assays. We found that individual mass was highly influential, heavier drones being more likely survive (4 h at 42°C) than smaller drones. In separate experiment, compared Northern California Southern response same 42°C), Californian - which enriched for African ancestry were originating from California. avoid survivor bias, conducted challenges using vitro assays remarkable variation resilience among sourced different commercial beekeeping operations, some exhibiting no reduction after others 75% viability. Further investigating potential causal factors such variation, association between assays, but virus inoculation (with Israeli acute paralysis virus) exacerbated negative effect These experiments establish vital framework understanding importance comorbidities sensitivity.

Language: Английский

Citations

0
The effects of zinc-methionine and Sel-Plex on the hypopharyngeal gland size, royal jelly yield and composition and the relative expression of hsp90 and trx gene in honey bees under heat stress DOI

Khalil Rasouli-Nadergoli,

Ali Asghar Sadeghi, P. Shawrang

et al.

Apidologie, Journal Year: 2025, Volume and Issue: 56(2)

Published: March 11, 2025

Language: Английский

Citations

0
Effect of honeybee queen size and HSP90 and HSC70 gene expression on thermal stress resistance DOI Creative Commons

Alireza Derafsh,

Abdolreza Salehi, Esmaeil Amiri

et al.

Frontiers in Bee Science, Journal Year: 2025, Volume and Issue: 3

Published: March 13, 2025

Introduction In beekeeping, queen cell size is a critical factor influencing the growth and development of bees. It was hypothesized that larger cells would produce queens with greater weight, enhanced resilience to heat stress, higher expression shock proteins ( HSP90 HSC70 ), leading improved survival under environmental stress. Materials methods This study, conducted in Shiraz Province, Iran, 2023, aimed test this hypothesis. A total 270 were divided into three groups based on size: large (10–9.5 mm), medium (9–8.5 small (7.5–7 mm). The reared using different methods: 1) simultaneous starter_finisher colonies, 2) separate 3) rearing presence queen. Since there types, resulted 90 for each method. Each group consisted subsets 30 cells: large, small, medium. From cells, 176 survived 94 died. Results discussion We stepwise procedure logistic model, results indicated which included type, method, birth showed best predictive performance. evidenced by lowest Akaike information criterion value. Then, from method 2, we placed 12 type two six subjected stress levels: low temperature 4°C high above 40°C. 36 frozen samples replicates treatment combination used molecular testing. Gene analysis real-time PCR evaluate gene expression. produced had significantly . These demonstrated superior rates high-temperature conditions compared smaller cells. Conclusions findings support hypothesis optimizing can enhance performance colony resilience. Our suggest promote development, resilience, rates, ultimately improving health productivity.

Language: Английский

Citations

0
N-acetylcysteine stimulates the proliferation and differentiation in heat-stressed skeletal muscle cells DOI
Jiawei Lu, Peng Zhao,

Xiuhu Ding

et al.

Journal of Thermal Biology, Journal Year: 2024, Volume and Issue: 124, P. 103958 - 103958

Published: Aug. 1, 2024

Language: Английский

Citations

3
Comparative assessment of food consumption, longevity, thermoregulation, and molecular health markers in mite-resistant and Italian honey bee stocks DOI Creative Commons
William G. Meikle, Milagra Weiss,

Daniela Adjaye

et al.

Apidologie, Journal Year: 2024, Volume and Issue: 55(3)

Published: April 25, 2024

Abstract Identifying traits for adaptation to different management and environmental regimes is key maintaining robust honey bee populations under global climate change. We compared mite-resistant (Pol-line Russian) Italian stocks in variable-temperature cage experiments (200 bees per cage) with respect food consumption, thermoregulation, gene expression, lifespan, 3 over 2 years. The stock consumed more syrup pollen on average than the stocks, but maintained higher cluster temperatures had median lifespans 8 days longer, consistent increased expression of vitellogenin relative stock. Model results indicated that, maintain same colony size as colonies would need about 13% sealed brood offset reduced worker lifespans. These differences among likely influence colony-level productivity health, showed importance experimental replication.

Language: Английский

Citations

2
Regulatory roles of long non-coding RNAs in short-term heat stress in adult worker bees DOI Creative Commons
Bing Zhang, Chaoying Zhang, Jiangchao Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 22, 2024

Abstract Long non-coding RNAs (lncRNAs) are crucial modulators of post-transcriptional gene expression regulation, cell fate determination, and disease development. However, lncRNA functions during short-term heat stress in adult worker bees poorly understood. Here, we performed deep sequencing bioinformatic analyses honeybee lncRNAs. RNA interference was by using siRNA targeting the most highly expressed lncRNA. The silencing effect on relative levels seven shock protein (HSP) genes, were subsequently examined. Overall, 7,842 lncRNAs 115 differentially (DELs) identified following exposure. Structural analysis revealed that overall abundance, length transcripts, exon number, open reading frames lower than those mRNAs. GO target genes mainly involved “metabolism,” “protein folding,” “response to stress,” “signal transduction” pathways. KEGG indicated processing endoplasmic reticulum” “longevity regulating pathway-multiple species” pathways enriched. Quantitative real-time polymerase chain reaction (qRT-PCR) detection selected DELs confirmed reliability data. Moreover, experiment feeding yielded a efficiency 77.51% for MSTRG.9645.5 . Upon this lncRNA, three HSP significantly downregulated ( p < 0.05), whereas other upregulated 0.05). Our results provide new perspective understanding regulatory mechanisms under stress.

Language: Английский

Citations

1
A mini-review on temperature-pathogen interactions and honey bee colony losses DOI
Hossam F. Abou‐Shaara, Martin Staroň, Ayman A. Owayss

et al.

Journal of Apicultural Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 10

Published: June 10, 2024

Language: Английский

Citations

1
VARIATIONS IN HEAT SHOCK PROTEINS BETWEEN DIFFERENT HONEY BEES AND BEE TAXA UTILIZING BIOINFORMATICS DOI Open Access
Hossam F. Abou‐Shaara

Uludağ Arıcılık Dergisi, Journal Year: 2024, Volume and Issue: 24(1), P. 38 - 52

Published: Feb. 10, 2024

The changes in climate and exposure to heat stress are major concerns for agricultural communities as it affects pollinators like bees. Bees from different taxa play a crucial role plant pollination, their induces the expression of shock proteins (HSPs) protect cells. Several studies have analyzed variations HSPs levels amino acid sequences. Databases sequences with molecular weights currently available. Variations been noted among individuals belonging same or bee exposed stress. properties could help understanding these variations. This study utilized bioinformatics protein analysis tools investigate 60 (HSP60) 83 (HSP83) 18 (15 Family Apidae, 2 Halictidae, one Megachilidae). showed some identical values bees genus Apis Bombus. For HSP60, all had high G content (587-602), followed by A (438-444), then C (389-404), finally T (282-291). HSP83, (730-759), (572-592), (406-419), (415-429). conserved domains were highly case HSP60 versus HSP83. motifs more families variation taxa. All hydrophilic variable isoelectric points. suggested an 3-D structure detected affecting response was discussed. paves way investigations on encourages use explain any observable

Language: Английский

Citations

0