Autophagy Impairment-Derived SQSTM1 Accumulation Promotes Ferroptosis in Corneal Epithelial Cells Through ACSL4 in Dry Eye DOI Creative Commons
Runze Zhang, Lihong Liang, Kai Liao

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(5), P. 23 - 23

Published: May 13, 2025

To reveal the function of autophagy impairment-derived sequestosome 1 (SQSTM1) in inducing ferroptosis an experimental dry eye model and investigate underlying mechanism. induce animal model, 8-week-old C57BL/6 mice were subcutaneously injected with scopolamine exposed to a desiccated environment. build vitro human corneal epithelial cells (HCECs) applied desiccating stress. Cell viability was examined using CCK-8 kit. Intracellular reactive oxygen species (ROS), oxidative lipid, Fe2+ detected H2DCFDA assay kit, C11 BODIPY probe, FerroOrange probe. Gene expression screened by RNA sequencing. Protein evaluated western blot immunofluorescence staining. Corneal defect area assessed fluorescein sodium Conjunctiva goblet counted periodic acid-Schiff Tear secretion measured phenol red cotton thread. Desiccating stress induced SQSTM1 accumulation both HCECs mice. knockdown alleviated HCECs. In contrast, overexpression promoted ferroptotic changes. Additionally, significantly increased acyl-CoA synthetase long chain family member 4 (ACSL4). Also, targeted inhibition ACSL4 mitigated symptoms caused The triggers through disease.

Language: Английский

Characterization of Mucosal Immune-Related lncRNAs and mRNAs in a Mouse Model of Allergic Conjunctivitis DOI Creative Commons
Hong Zhang, Hongyu Zhang,

Qing Leng

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 6061 - 6076

Published: May 1, 2025

Allergic conjunctivitis (AC) is a common inflammatory condition characterized by immune dysregulation in response to environmental allergens. Despite extensive research into general allergic mechanisms, the specific immunological features of ocular mucosal microenvironment remain poorly understood. Investigating immune-related mRNAs and LncRNAs may provide insights mechanisms underlying AC potential novel targets for therapeutic intervention. An model was established using female BALB/c mice sensitized with ragweed pollen. Conjunctival tissues from control groups were pooled RNA extraction, followed Illumina sequencing. Differential gene expression identified DESeq2, functional enrichment analyzed GO, KEGG, GSEA. RT-qPCR validated results, while Human Protein Atlas used assess protein expression. A murine successfully established, confirmed progressively increasing clinical scores significantly elevated scratching frequency. Transcriptomic analysis revealed significant differences lncRNAs between groups. GO indicated that both upregulated downregulated genes enriched biological processes related stimulus, system processes, signaling, metabolic processes. KEGG showed pathways such as steroid hormone biosynthesis, histidine metabolism, glycolysis/gluconeogenesis, IL-17 involved cytokine-cytokine receptor interaction hematopoietic cell lineage. GSEA pathways, including MAPK, STAT1, STAT2. Mucosal immunity-related Bpifa1, Lcn2, Reg3g AC. Co-expression also several lncRNAs, Stoml3-202 Etohd2-205. This study first systematically analyze AC, identifying immunity molecules like Bpifa1 Reg3g. These findings underscore unique involvement new modulation allergy treatment.

Language: Английский

Citations

0
Autophagy Impairment-Derived SQSTM1 Accumulation Promotes Ferroptosis in Corneal Epithelial Cells Through ACSL4 in Dry Eye DOI Creative Commons
Runze Zhang, Lihong Liang, Kai Liao

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(5), P. 23 - 23

Published: May 13, 2025

To reveal the function of autophagy impairment-derived sequestosome 1 (SQSTM1) in inducing ferroptosis an experimental dry eye model and investigate underlying mechanism. induce animal model, 8-week-old C57BL/6 mice were subcutaneously injected with scopolamine exposed to a desiccated environment. build vitro human corneal epithelial cells (HCECs) applied desiccating stress. Cell viability was examined using CCK-8 kit. Intracellular reactive oxygen species (ROS), oxidative lipid, Fe2+ detected H2DCFDA assay kit, C11 BODIPY probe, FerroOrange probe. Gene expression screened by RNA sequencing. Protein evaluated western blot immunofluorescence staining. Corneal defect area assessed fluorescein sodium Conjunctiva goblet counted periodic acid-Schiff Tear secretion measured phenol red cotton thread. Desiccating stress induced SQSTM1 accumulation both HCECs mice. knockdown alleviated HCECs. In contrast, overexpression promoted ferroptotic changes. Additionally, significantly increased acyl-CoA synthetase long chain family member 4 (ACSL4). Also, targeted inhibition ACSL4 mitigated symptoms caused The triggers through disease.

Language: Английский

Citations

0