Camellia sinensis methanolic leaves extract: Phytochemical analysis and anticancer activity against human liver cancer cells

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0309795 - e0309795

Published: Nov. 14, 2024

Background The study’s primary goal is to ascertain whether there a relationship between the processed green tea methanolic extract’s (GTME) phytochemical components and its potential effectiveness against human liver cancer cells. GTME’s composition was identified using gas chromatography-mass spectrometry, capacity lower cellular proliferation cause apoptosis in HepG2 cancerous cell lines checked. Results findings of chromatogram showed that GTME included bioactive antioxidants anticancer substances. Additionally, utilizing MTT, comet assay, acridine revealed selective cytotoxic impact with significant IC 50 value (27.3 µg/ml) on cells without any harmful effects WI-38 healthy Also, compared untreated cells, extract-treated had an upsurge proportion have undergone displayed nucleus, which sign DNA damage. In addition, treated stop G1 phase sub-G1 apoptotic (37.32%) flow cytometry analysis. Furthermore, reactive oxygen species were shown be responsible for apoptosis, tested extract significantly reduced their levels Lastly, changed protein expression linked cycle arrest apoptosis. Conclusion These provided information about processes through inhibited growth HepG2. Therefore, it has as effective natural therapy treatment cancer. However, validate these findings, animal models must used vivo studies.

Language: Английский

---

GC–MS analysis and in silico approaches to Stichopus hermanii as anti-inflammatory through PKC-β inhibition

Results in Chemistry, Journal Year: 2025, Volume and Issue: 14, P. 102086 - 102086

Published: Feb. 1, 2025

Language: Английский

---

Eco-friendly and efficient extraction of phenolic compounds from Commiphora gileadensis bark using microwave-assisted extraction

Journal of Industrial and Engineering Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Language: Английский

---

Optimized production of carboxymethyl cellulose/guar gum based durable hydrogel for in vitro performance assessment

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135121 - 135121

Published: Aug. 27, 2024

Language: Английский

---

Phytochemical Compounds in Sea Cucumber Have the Potential to Reduce Oxidative Stress and Inflammation Due to Exercise: Systematic Review

Physical Education Theory and Methodology, Journal Year: 2024, Volume and Issue: 24(1), P. 158 - 168

Published: Feb. 29, 2024

Study purpose. This study aims to analyze and highlight the potential of Sea Cucumber in reducing oxidative stress inflammation caused by exercise. Materials methods. The is a type systematic review research using searches from various journal databases such as Science Direct, Pubmed Web Science. inclusion criteria this were journals published last 5 years which discussed sea cucumber, stress, Furthermore, exclusion are that not reputable or indexed Scopus A total 1038 articles identified. 8 met selected analyzed for review. For standard operations, followed Preferred Reporting Items Systematic reviews Meta-Analyses (PRISMA) assessment. Results. results report strong phenolic content found has anti-oxidant properties may reduce stress. anti-inflammatory cucumbers have uncontrolled intense Conclusions. In addition, case, an effect suppressing secretion pro-inflammatory cytokines. Through review, we recommend future studies should perform clinical trials on effects cucumber biomarkers inflammatory after

Language: Английский

---

Extraction and Characterization of Biological Phytoconstituents of Commiphora gileadensis Leaves Using Soxhlet Method

Processes, Journal Year: 2024, Volume and Issue: 12(8), P. 1567 - 1567

Published: July 26, 2024

Commiphora gileadensis is a medicinal plant with wide range of biological characteristics. Many medical diseases can be treated using the leaves C. gileadensis, including bacterial infections, inflammatory illnesses, and wounds. As result, Soxhlet extraction method was used to extract phenolic components measure recovery yields from leaf. The impacts parameters (extraction time 30–150 min, sample/solvent ratio 1:20–1:40 g/mL, concentration ethanol solvent 20–100% v/v) on total flavonoid content (TFC), (TPC), yield were investigated one-factor-at-a-time (OFAT) technique. Fourier transform infrared spectroscopy (FTIR) gas chromatography–mass (GC–MS) analyses have been employed evaluate extracts for presence various phytochemicals. According results, leafs ethanolic extracted via process achieved maximum at 90 min time, feed/solvent 1:30 40% v/v concentration. These were: = 23.20 ± 0.10% w/w, TPC 59.93 1.33 mg GAE/g d.w., TFC 19.65 1.77 QE/g d.w.. Further, 20 excellent antioxidant characteristics found in leaf method.

Language: Английский

---

Novel Erodium glaucophyllum (L.) Aiton growing in arid environment: Phytochemical characterization, antimicrobial, antioxidant, and anticancer potential

Electronic Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

---

Antioxidant and antiproliferative activity of Portulaca Oleracea and Azanza Garckeana on cervical cancer

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Background: Mortality and incidence rates of cervical cancer are on the rise in Sub-Saharan Africa. Current management strategies not fully effective as they bring about major side effects issues such drug resistance. Indigenous plants have been used ethnomedicine to treat conditions related cancer. Aim: The present study was aimed at evaluating antioxidant anti-proliferation potential extracts derived from Portulaca oleracea Azanza garckeana plants. Methods: Phytochemicals were extracted all plant parts two with methanol, ethanol, chloroform, distilled water, isopropanol hexane. Qualitative phytochemical analysis conducted. total phenolic flavonoid content estimated, activity determined using DPPH (2,2-diphenyl-1-picryl hydrazyl) ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) assays. antiproliferative HeLa cells analysed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Results: P. A. stem leaf methanolic extract had high between 14.25 16.39 TE mg/100 g 41.70 52.03 QE g. Methanolic ethanolic displayed anti-proliferative towards affected cell viability a dose dependent fashion ranging 69 92% reduction 400 µg/ml concentration. IC₅₀ values ranged 242.09 329.31 µg/mL 22.78 ± 10.37 for cisplatin. Conclusions: garckeana demonstrate activity, which indicate these anticancer development.

Language: Английский

---

The Cabotage Principle on Law Enforcement for Licensing Ship Operations in Indonesian Waters

Journal of Human Rights Culture and Legal System, Journal Year: 2023, Volume and Issue: 3(3), P. 678 - 705

Published: Nov. 20, 2023

This study aims to examine and comprehend the cabotage principle on law enforcement for licensing ship operations in Indonesian Waters. is a descriptive analysis of normative legal research.  Secondary data, comprising primary, secondary, tertiary materials, utilized. Techniques collecting data involve library research, whereas techniques analyzing interpretation. The results show that Indonesia has regulated several regulations. However, supervision applying this not been optimal due increase transactions between countries globalization overlapping shipping permit issues marked by authority 13 (thirteen) mandated ministries/state institutions 17 (seventeen) laws as officers marine coastal areas. overlap referred characterized same carry out supervision, pursuit, investigation Indonesia's maritime jurisdictional

Language: Английский

---

Length-Weight Relationship and Condition Factors of Sea Cucumber on Mare and Moti Islands Conservation Areas in North Maluku

Jurnal Kelautan Tropis, Journal Year: 2024, Volume and Issue: 27(1), P. 150 - 160

Published: March 6, 2024

This work aimed at determining the growth patterns and condition factors of three sea cucumber species, namely Holothuria atra, Bohadschia marmomata, B. vitiensis in seagrass beds Mare Moti Islands. Samples were collected March 2023 using a roaming survey method block area with an 5000 m2. As amount 54 cucumbers analysed from Island 58 individuals Island. The results this study indicate that correlation (r) length-weight relationship for species is stronger (r > 0.6) on than H. atra marmorata have negative allometric pattern, while has positive pattern both islands. Fulton assessed recorded 2.84 ± 2.01, 3.13 2.47 9.24 5.73. Meanwhile, found 12.49 11.66, 9.58 7.38, 8.87 3.68. indicated all are good physical condition. relative weight factor (Wr) islands range above 100, which food surplus their habitat. research can be important as preliminary data terms sustainable management stocks North Maluku.

Language: Английский

---