Risk of adverse cardiovascular events based on common genetic variants in 8-year follow-up of the LIPIDOGEN2015 population using the polygenic risk score (PRS) - study design and methodology. DOI Creative Commons
Maciej Banach, Martyna Fronczek, Tadeusz Osadnik

et al.

Archives of Medical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Introduction Classical risk factors such as hypertension, hypercholesterolemia, pre-diabetes, diabetes and obesity can predict adverse cardiovascular events, but they are less prognostic in patients age <60 years. Polygenic scores (PRS) be effective predicting coronary events younger middle-aged patients. Our main aim is to assess the utility of new PRS created for Polish population mortality during an 8-year follow-up a nationwide LIPIDOGEN2015 population. Material methods All DNA samples 1779 were genotyped using Infinium Global Screening Array-24+ v3.0 Kit microarrays. The amplified, fragmented, hybridized BeadChips. BeadChips scanned iScan converted genotypes Genome Studio 2.0. Results We will develop based on marked single nucleotide polymorphisms (SNPs) project's studied determine analyzed group's death due diseases (CVD) data obtained from 8-years patient-follow-up. Based developed scale biochemical analyses, we effectiveness lipid-lowering therapy with statins high low genetic sudden CVD (secondary endpoints). Conclusions scale, combined clinical covariates, facilitate creation algorithm long-term mortality. This enable us stratify more precisely, which may result earlier implementation lifestyle changes dietary adjustments potentially initiate pharmacotherapy at-risk individuals.

Language: Английский

Pyruvate dehydrogenase alleviates macrophage autophagy in Hcy-induced ApoE&lt;sup&gt;-/-&lt;/sup&gt; mice DOI Creative Commons

Qiujun Liu,

Li Feng,

Shutong Hu

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Macrophages play a protective role in atherosclerosis, whereas homocysteine (Hcy) is recognized as an independent risk factor for atherosclerosis. Defects macrophage autophagy contribute to the formation of atherosclerotic plaques, and dysregulated energy metabolism closely linked process autophagy. However, regulation by pyruvate dehydrogenase (PDH), key component PDH complex involved metabolic homeostasis, remains poorly understood context atherosclerosis induced Hcy. In our study, proteomic profiling identifies 748 upregulated proteins 760 downregulated Hcy-treated macrophages. KEGG pathway analysis reveals significant enrichment differentially expressed metabolism-related pathways, including those related biosynthesis amino acids, carbon metabolism, glycolysis/gluconeogenesis. Additionally, we explore mediating Hcy-induced ApoE -/- mice. The results show marked reduction expression activity macrophages, leading impaired Notably, activation enhances assembly initiator ULK1-FIP200-Atg13 through modulation AMPK/mTOR signaling pathway, suggesting potential therapeutic target

Language: Английский

Citations

0
The mitochondrial dysfunction, alongside the modifiable burden of traditional risk factors, drives the development of early-onset coronary artery disease DOI Creative Commons
Jonica Campolo, Paola Canale, Gianluca Gazzaniga

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 19, 2025

Mitochondrial dysfunction is associated with increased risk of atherosclerosis by disrupting key cellular processes that contribute to premature vascular ageing. However, the specific role mitochondrial in early-onset coronary artery disease (EOCAD), which increasing at a particularly alarming rate, remains largely unexplored. This study investigated association leukocyte mtDNA-CN and mtDNA4977 deletion EOCAD. The included 118 patients (99 men, 51.0 ± 5.6 years) angiographically EOCAD (≤60 150 healthy controls (94 49.8 5.8 years). Quantitative RT-PCR was used quantify rate. group had higher prevalence male gender (p < 0.001), smoking = hypertension diabetes mellitus 0.04) obesity 0.001) than controls. lower 0.026). Low levels were significantly 0.004), 0.039), hypercholesterolemia 0.001). Increased males 0.026) hypercholesterolemic ROC curve predicting showed an AUC 0.902 (95% CI 0.867-0.937, p 0.762 0.691-0.834, respectively. Logistic regression analysis adjusted for confounders both independent significant predictors 0.001 0.001, respectively). characterized high modifiable factors damage, underscoring need more efforts reduce burden traditional highlighting potential innovative mitochondrial-targeted therapies.

Language: Английский

Citations

0
The prevalence, patients' characteristics, and hyper-Lp(a)-emia risk factors in the polish population. The first results from the PMMHRI-Lp(a) registry DOI Creative Commons
Bożena Sosnowska, Joanna Lewek, Weronika Adach

et al.

Progress in Cardiovascular Diseases, Journal Year: 2024, Volume and Issue: 86, P. 54 - 61

Published: Aug. 25, 2024

The knowledge on the prevalence of elevated lipoprotein(a) (Lp(a)), patients' characteristics, and nongenetic risk factors is scarce in some regions including Poland, largest Central Eastern European country. Thus, we aimed to present results from Lp(a) registry established Poland's 2nd largest, supra-regional hospital - Polish Mother's Memorial Hospital Research Institute (PMMHRI).

Language: Английский

Citations

3
New data allow to better understand the secrets of lipoprotein(a): is that for sure? DOI Creative Commons
Stanisław Surma, Bożena Sosnowska, Željko Reiner

et al.

European Heart Journal Open, Journal Year: 2024, Volume and Issue: 4(5)

Published: Aug. 3, 2024

Language: Английский

Citations

1
Risk of adverse cardiovascular events based on common genetic variants in 8-year follow-up of the LIPIDOGEN2015 population using the polygenic risk score (PRS) - study design and methodology. DOI Creative Commons
Maciej Banach, Martyna Fronczek, Tadeusz Osadnik

et al.

Archives of Medical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Introduction Classical risk factors such as hypertension, hypercholesterolemia, pre-diabetes, diabetes and obesity can predict adverse cardiovascular events, but they are less prognostic in patients age <60 years. Polygenic scores (PRS) be effective predicting coronary events younger middle-aged patients. Our main aim is to assess the utility of new PRS created for Polish population mortality during an 8-year follow-up a nationwide LIPIDOGEN2015 population. Material methods All DNA samples 1779 were genotyped using Infinium Global Screening Array-24+ v3.0 Kit microarrays. The amplified, fragmented, hybridized BeadChips. BeadChips scanned iScan converted genotypes Genome Studio 2.0. Results We will develop based on marked single nucleotide polymorphisms (SNPs) project's studied determine analyzed group's death due diseases (CVD) data obtained from 8-years patient-follow-up. Based developed scale biochemical analyses, we effectiveness lipid-lowering therapy with statins high low genetic sudden CVD (secondary endpoints). Conclusions scale, combined clinical covariates, facilitate creation algorithm long-term mortality. This enable us stratify more precisely, which may result earlier implementation lifestyle changes dietary adjustments potentially initiate pharmacotherapy at-risk individuals.

Language: Английский

Citations

0